DLL Bioengineering

Cellular culture and observation, biocompatibility, biomaterials, biomechanics

Biology is getting more and more involved in other fields such as mechanics, material sciences, micro-engineering, physics…
 
Mastering cell culture techniques, testing viability and biocompatibility, and working in sterile environments with living organisms, are the goals of the hands-on training given at the DLL-Bioengineering.

Equipement and user guide

  • 4 double laminar flows
  • 2 CO2 incubators
  • 2 bacteria incubators (RT to 80 °C)
  • 1 adjustable temperature bacteria stirrer (4 °C to 65 °C)
  • 1 Thermomixer with blocks for 50 ml tubes and 1.5 ml microtubes (15 °C to 100 °C)
  • 1 refrigerated centrifuge with buckets for 15 and 50 ml tubes as well as buckets for microplates. Max speed 4000 rpm
  • 1 refrigerated centrifuge for 18 microtubes. Max speed 14000 rpm
  • Several bench centrifuges for 12 microtubes. Max speed 13500 rpm
  • Several precision balances (max. Capacity: 3200 g / readability: 0.01 g)
  • Several analytical balances (max. Capacity: 220 g / readability: 0.1 mg)
  • 2 power supplies (300V / 700mA / 150w)
  • 1 EasyCast tank for agarose gel (7 x 8 cm)
  • 1 EasyCast tank for agarose gel (12 x 14 cm)
  • Equipment for the manufacture and migration of acrylamide gels
  • Western blot transfer material
  • Agarose gel documentation system with UV transilluminator (312 nm) (E-Box)
  • 1 microplate reader: reading fluorescence, luminescence and absorbance
  • 1 Nanodrop: Proteins, DNA, RNA
  • Several inverted and upright microscopes with phase contrast and fluorescence equipped with camera and tablet
  • Nikon Eclipse Ti2 platform
  • Nanolive
  • 96-well plates with 3 independent temperature zones
  • Several fridges, -20 ° C freezers and storage space in a -80 ° C freezer
  • 1 pH meter
  • 1 autoclave for liquids (max volume 500 ml)
  • 1 microwave
  • Several sets of pipettes (from 2 μl to 1 ml)
  • Several Vortices
  • Several Pipet-boys
  • 1 chemical hood
  • 1 UV lamp (8 Watt / 365 nm), mobile
  • Glassware

Access and safety rules

Training on equipment

Reservation https://reservations.epfl.ch, after validation of the safety training.

Fill up the logbook of the equipment after each time you use it.

Cours Description Cours Description

ME-380

Méthodes expérimentales en biomécanique

MICRO-562

Biomicroscopy II

MSE-471

Biomaterials (for MX)

BIO-318 (iGEM)

International Genetically Engineered Machine competition

MSE-360

Science des polymères

SensUs

International competition on biosensors for health

ENV-202

Microbiologie pour l’ingénieur