DLL Bioengineering
Cellular culture and observation, biocompatibility, biomaterials, biomechanics
Biology is getting more and more involved in other fields such as mechanics, material sciences, micro-engineering, physics…
Mastering cell culture techniques, testing viability and biocompatibility, and working in sterile environments with living organisms, are the goals of the hands-on training given at the DLL-Bioengineering.
Equipement and user guide
- 4 double laminar flows
- 2 CO2 incubators
- 2 bacteria incubators (RT to 80 °C)
- 1 adjustable temperature bacteria stirrer (4 °C to 65 °C)
- 1 Thermomixer with blocks for 50 ml tubes and 1.5 ml microtubes (15 °C to 100 °C)
- 1 refrigerated centrifuge with buckets for 15 and 50 ml tubes as well as buckets for microplates. Max speed 4000 rpm
- 1 refrigerated centrifuge for 18 microtubes. Max speed 14000 rpm
- Several bench centrifuges for 12 microtubes. Max speed 13500 rpm
- Several precision balances (max. Capacity: 3200 g / readability: 0.01 g)
- Several analytical balances (max. Capacity: 220 g / readability: 0.1 mg)
- 2 power supplies (300V / 700mA / 150w)
- 1 EasyCast tank for agarose gel (7 x 8 cm)
- 1 EasyCast tank for agarose gel (12 x 14 cm)
- Equipment for the manufacture and migration of acrylamide gels
- Western blot transfer material
- Agarose gel documentation system with UV transilluminator (312 nm) (E-Box)
- 1 microplate reader: reading fluorescence, luminescence and absorbance
- 1 Nanodrop: Proteins, DNA, RNA
- Several inverted and upright microscopes with phase contrast and fluorescence equipped with camera and tablet
- Nikon Eclipse Ti2 platform
- Nanolive
- 96-well plates with 3 independent temperature zones
- Several fridges, -20 ° C freezers and storage space in a -80 ° C freezer
- 1 pH meter
- 1 autoclave for liquids (max volume 500 ml)
- 1 microwave
- Several sets of pipettes (from 2 μl to 1 ml)
- Several Vortices
- Several Pipet-boys
- 1 chemical hood
- 1 UV lamp (8 Watt / 365 nm), mobile
- Glassware
Access and safety rules
Training on equipment
Reservation https://reservations.epfl.ch, after validation of the safety training.
Fill up the logbook of the equipment after each time you use it.
| Cours | Description | Cours | Description |
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Méthodes expérimentales en biomécanique |
Biomicroscopy II |
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Biomaterials (for MX) |
International Genetically Engineered Machine competition |
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Science des polymères |
International competition on biosensors for health |
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Microbiologie pour l’ingénieur |