The BIOP hosts over 20 light microscopes with imaging modalities ranging from stereomicroscopy to super-resolution. Below you will find a (non-exhaustive) list of the techniques available on the platform.
FCS is a correlation analysis of fluctuation of the fluorescence intensity. The analysis provides parameters of the physics under the fluctuations. One of the interesting applications of this is an analysis of the concentration fluctuations of fluorescent particles (molecules) in solution.
Available Microscopes: Zeiss LSM710
Contact: Arne Seitz
Fluorescence Lifetime Imaging Microscopy (FLIM) uses dedicated hardware that enables the calculation of the time between a fluorophore being excited and when a photon is released, called the Lifetime). They are often used in FRET (Förster resonance energy transfer) experiments.
Available Microscopes: Leica SP8 FLIM
FRAP-like experiments are used to quantify the mobility of proteins in cells or tissues. They require high laser power and scanners to bleach rapidly and precisely the fluorophores of interest.
Live imaging systems are usually inverted microscopes with temperature, humidity and CO2 control in order to host delivate live samples for long-term imaging.
Microdissection microscopes utilize pulsed UV lasers to perform localized micro-ablations in tissue or within living cells. This can be used for isolating fresh tissue samples for RT-PCR or altering biological processes in cells/organisms in order to study their influence.
Super-resolution techniques have gained momentum over the last few years, pushing the limits of light microscopy beyond the classical diffraction limit.
At the BIOP, you can perform Structured Illumination (SIM), Stimulated Emission Depletion (STED) and Stochastic Optical Reconstruction (STORM).
Whole Slide Scanners allow the acquisition of large areas in minimum time and high magnification (up to 40x). Data can then be viewed and processed in dedicated software, such as QuPath.
The BIOP offers two fully automated whole slide scanners with 100-slide capacity. Imaging modalities include brightfield and fluorescence. For Confocal tile scanning, our new Leica SP8 Microscopes with Navigator are a viable option as well.