NIKON CSU W1

Confocal spinning disk microscope beam homogenizer, FRAP, TIRF and STORM module.

Nikon Spinning Disk CSU W1. Fast confocal (dual camera) with environmental control (temperature and CO2). Includes illumination homogeneizer for large FOV acquisitions with limited vignetting, hardware autofocus (Perfect Focus System), and additional FRAP, TIRF and STORM modules.

Person in charge: Nicolas Chiaruttini

Book on SV-BBS


Technical Specifications

Imaging: Confocal, FRAP & STORM
Stand:
Inverted Nikon Eclipse Ti2-E motorized
XY-Stage: Motorized
Illumination: LED Lamp, Laser
Software: NIS Element AR

Detectors

Camera(2x) Photometrics
Prime 95B
Andor iXON Ultra
Chip TechnologysCMOS GrayscaleEMCCD Grayscale
Sensor Format 1200 x 12001024 x 1024
Chip size13.2 mm x 13.2 mm13.3 mm x 13.3 mm
Pixel size11 um x 11 um13 um x 13 um
Dynamic Range16-bit16-bit
C-mount1x1x
Optovar1x – 1.5x 1x – 1.5x
Performance certificatedownloaddownload

Objectives

ObjectiveMag/NAMediumContrastWD (mm)
CFI Plan Apo Lambda10x/0.45air4
CFI Plan Apo Lambda20x/0.75air1
CFI Plan Apo Lambda60x/1.40oil0.13
SR HP Apo TIRF100x/1.49oil0.12

Laser Lines

WavelengthLaser Type
405 nmDiode
445 nmDiode
488 nmDiode
514 nmDiode
561 nmDiode
638 nmDiode

Emission Filtercubes in Spinning Disk

EMISSION FILTER WHEEL 1
NameEmissionNo
542/27BP 542/27BrightLine 542/27
600/52BP 600/52BrightLine 600/52
708/75BP 708/75BrightLine 708/75
EMISSION FILTER WHEEL 2
NameEmissionNo
447/60BP 447/60BrightLine 447/60
472/30BP 472/30BrightLine 472/30
525/50BP 525/50BrightLine 525/50
542/27BP 542/27BrightLine 542/27
600/52BP 600/52BrightLine 600/52

NB : the system is not equipped with an LED epi-illumination system : the focus has to be made in brightfield or direct with the camera.

Tutorial