Olympus Cell xCellence

Olympus Cell xCellence
Inverted widefield microscope for transmission and fluorescence live imaging boasting CCD and EMCCD cameras, full CO2/Temperature controls and fast filter switching for live acquisitions.

Olympus inverted microscope. Full CO2 and temperature control for live imaging. Fluorescence and Brightfield.

Person in charge: José Artacho

Book on SV-BBS

Starting Temperature and CO2

Please make sure that you turn on the CO2 valve for your system. They are located on the leftmost wall from the Cell xCellence.

Remember to switch your CO2 valve off after you are done with your session if no other user is present


Technical Specifications

Stand: Inverted Olympus IX81 motorized
XY-Stage: Motorized
Illumination: MT20 with 150W Xenon lamp
Software: CellSens

Detectors

CamerasHamamatsu ORCA 03gAndor Ixon 3
Chip Technology CCD Grayscale EMCCD Grayscale
Sensor Format 1344 x 1024512 x 512
Chip size8.67mm x 6.60mm8.2 mm x 8.2 mm
Pixel size6.45 um x 6.45 um16.0 um x 16.0 um
Dynamic Range12-bit14-bit (max)
C-mount1.0x1.0x
Optovar1.0x – 1.6x1.0x – 1.6x

Objectives

ObjectiveMag/NAMediumContrastWD (mm)
UPLFLN10x/0.3airPH19.50
U PL SAPO20x/0.75air—-
LUC PL FLN40x/0.60airPH23.0 – 4.2
UPLAN APO40x/1.0oiliris PH30.12
UPLFLN4x/013airPHL17
UPL S APO60x/1.35oil0.15

Filtercubes

The links in the table below will send you to an FPbase configuration page.
Note: The excitation/emission filters can be combined in any way, because they are in two separate motorized filterwheels.

NameExcitationDichroicEmissionNo
DAPIBP 387/11410/504/582/669BP 440/30
FITCBP 485/20410/504/582/669BP 525/30
Cy3BP 560/25410/504/582/669BP 607/36
Cy5BP 650/13410/504/582/669BP 684/24
Fura 340BP 340/12400BP 525/30
Fura 380BP 380/14400BP 525/30

Filtercubes for Eyepiece

NameExcitationDichroicEmissionNo
visDAPIBP 387/11410BP 440/30
visFITCBP 485/20504BP 525/30
visCy3BP 560/25582BP 607/36

Tutorials, Documentation

C4Science

Documentation on our C4Science page