Olympus inverted microscope. Full CO2 and temperature control for live imaging. Fluorescence and Brightfield.
Person in charge: José Artacho
Book on SV-BBS
Technical Specifications
Stand: Inverted Olympus IX81 motorized
XY-Stage: Motorized
Illumination: MT20 with 150W Xenon lamp
Software: CellSens
Detectors
| Cameras | Hamamatsu ORCA 03g | Andor Ixon 3 |
| Chip Technology | CCD Grayscale | EMCCD Grayscale |
| Sensor Format | 1344 x 1024 | 512 x 512 |
| Chip size | 8.67mm x 6.60mm | 8.2 mm x 8.2 mm |
| Pixel size | 6.45 um x 6.45 um | 16.0 um x 16.0 um |
| Dynamic Range | 12-bit | 14-bit (max) |
| C-mount | 1.0x | 1.0x |
| Optovar | 1.0x – 1.6x | 1.0x – 1.6x |
Objectives
| Objective | Mag/NA | Medium | Contrast | WD (mm) |
| UPLFLN | 10x/0.3 | air | PH1 | 9.50 |
| LUC PL FLN | 20x/0.45 | air | PH1 | 6.60 – 7.80 |
| LUC PL FLN | 40x/0.60 | air | PH2 | 3.0 – 4.2 |
| UPLAN APO | 40x/1.0 | oil | PH2 | 0.12 |
| UPL S APO | 60x/1.2 | water | – | 0.28 |
| UPL S APO | 60x/1.35 | oil | – | 0.15 |
Filtercubes
The links in the table below will send you to an FPbase configuration page.
Note: The excitation/emission filters can be combined in any way, because they are in two separate motorized filterwheels.
| Name | Excitation | Dichroic | Emission | No |
| DAPI | BP 387/11 | 410/504/582/669 | BP 440/30 | – |
| FITC | BP 485/20 | 410/504/582/669 | BP 525/30 | – |
| Cy3 | BP 560/25 | 410/504/582/669 | BP 607/36 | – |
| Cy5 | BP 650/13 | 410/504/582/669 | BP 684/24 | – |
| Fura 340 | BP 340/12 | 400 | BP 510/40 | – |
| Fura 380 | BP 380/14 | 400 | BP 510/40 | – |