Olympus inverted microscope. Full CO2 and temperature control for live imaging. Fluorescence and Brightfield.
Person in charge: José Artacho
Book on SV-PPMS
Starting Temperature and CO2
Please make sure that you turn on the CO2 valve for your system. They are located on the leftmost wall from the Cell xCellence.
Remember to switch your CO2 valve off after you are done with your session if no other user is present
Technical Specifications
Stand: Inverted Olympus IX81 motorized
XY-Stage: Motorized
Illumination: MT20 with 150W Xenon lamp
Software: CellSens
Detectors
| Cameras | Hamamatsu ORCA 03g | Andor Ixon 3 |
| Chip Technology | CCD Grayscale | EMCCD Grayscale |
| Sensor Format | 1344 x 1024 | 512 x 512 |
| Chip size | 8.67mm x 6.60mm | 8.2 mm x 8.2 mm |
| Pixel size | 6.45 um x 6.45 um | 16.0 um x 16.0 um |
| Dynamic Range | 12-bit | 14-bit (max) |
| C-mount | 1.0x | 1.0x |
| Optovar | 1.0x – 1.6x | 1.0x – 1.6x |
Objectives
| Objective | Mag/NA | Medium | Contrast | WD (mm) |
| UPLFLN | 10x/0.3 | air | PH1 | 9.50 |
| U PL SAPO | 20x/0.75 | air | —- | |
| LUC PL FLN | 40x/0.60 | air | PH2 | 3.0 – 4.2 |
| UPLAN APO | 40x/1.0 | oil | iris PH3 | 0.12 |
| UPLFLN | 4x/013 | air | PHL | 17 |
| UPL S APO | 60x/1.35 | oil | – | 0.15 |
Filtercubes
The links in the table below will send you to an FPbase configuration page.
Note: The excitation/emission filters can be combined in any way, because they are in two separate motorized filterwheels.