NIKON Stereology

Nikon Stereology
Upright stereology-centered microscope with motorized stage and StereoInvestigator software.

Nikon Stereology Upright Microscope. Suitable for tiling in fluorescence and brightfield.

Person in charge: Arne Seitz

Book on SV-PPMS


Technical Specifications

Stand: Upright Nikon Eclipse 90i motorized
XY-Stage: Motorized
Illumination: Halogen Lamp
Software: Stereo Investigator & NIS-Elements

Detectors

CamerasQ-imaging RETIGA-2000RNikon DS Qi2
Chip TechnologyCCD ColorCMOS Grayscale
Sensor Format 1600 x 12001636 x 1088
Chip size11.84 mm x 8.88 mm36 mm x 23 mm
Pixel size7.4 um x 7.4 um7.3 um x 7.3 um
Dynamic Range12-bit12-bit
C-mount1.0x2.5x

Objectives

ObjectiveMag/NAMediumContrastWD (mm)
CFI PLAN APOCHROMAT2x/0.1air8.50
CFI PLAN APOCHROMAT10x/0.45air4.00
CFI PLAN APOCHROMAT20x/0.75air1.00
CFI PLAN APOCHROMAT VC60x/1.4oil0.13

Filtercubes

The links in the table below will send you to an FPbase configuration page.

NameExcitationDichroicEmissionNo
BlueBP 387/11FT 409BP 447/60Semrock 1160B
GreenBP 466/40FT 495HC 525/50Semrock 4050B
Gold HCBP 534/20FT 552BP 572/28AHF – SP. GOLD HC
RedBP 562/40FT 593BP 624/40Semrock Tx-Red 4040C
Far-
Red
BP 628/40FT 660BP 692/40Semrock Cy5 4040C
Near Infrared408/504/581/667/762440/521/607/694/809Semrock Penta

Tutorial