NIKON CrestOptics

Nikon CrestOptics X-Light V3. Fast spinning disk confocal (dual camera) and DeepSim module with environmental control (temperature and CO2). Includes illumination homogeneizer for large FOV acquisitions with limited vignetting, hardware autofocus (Perfect Focus System), and additional super resolution SIM module.

 

Person in charge: Nicolas Chiaruttini

Book on SV-PPMS


Technical Specifications

Imaging: Widefield, Confocal, SIM
Stand:
Inverted Nikon Eclipse Ti2 motorized
XYZ-Stage: Motorized
Illumination: LED Lamp (CoolLed pE 800), Laser (Lumencor Celesta 7 NII-AA)
Software: NIS Element AR

Spinning Disk geometry: 50/250 µm (diameter/spacing)

Detectors

CameraTeledyne Kinetix M-CTeledyne Kinetic M-C
Chip TechnologysCMOS GrayscalesCMOS Grayscale
Sensor Format 3200 x 32003200 x 3200
Chip size29 mm (diagonal)29 mm (diagonal)
Pixel size6.5 um x 6.5 um6.5 um x 6.5 um
Dynamic Range16-bit16-bit
F-mount1x1x
Optovar1x – 1.5x1x – 1.5x

Objectives

ObjectiveMag/NAMediumContrastWD (mm)
Plan Apo Lambda D10x/0.45airDIC4
Plan Apo Lambda D20x/0.80airDIC0.8
Plan Apo Lambda S25x/1.05SILDIC0.55
Plan Apo Lambda S40x/1.25waterDIC0.16 – 0.20
Plan Apo Lambda D60x/1.42oilDIC0.15

Laser Lines

WavelengthLaser Type
395 nmSolid-state laser type 4
440nmSolid-state laser type 4
488 nmSolid-state laser type 4
514 nmSolid-state laser type 4
545 nmSolid-state laser type 4
640 nmSolid-state laser type 4
730 nmSolid-state laser type 4

Filtercubes in Microscope Turret

The filters below are used when looking for your sample only through the oculars.

Excitation and Emission Filters in Spinning Disk and SIM

EXCITATION FILTER
NameExcitationNo
DA/FI/TR/Cy5/Cy7Penta Bandpass (391/477/549/639/741)BrightLine Penta bandpass

Tutorial