Zeiss LSM980

Zeiss LSM980 at the BIOP
AiryScan enabled confocal microscope with Colibri lightsource

Zeiss LSM 980 Inverted microscope. Multi-purpose confocal with 32 Channels AiryScan, tPMT, widefield & brightfield capability. Motorized stage.

Persons in charge: Nicolas Chiaruttini & Thierry Laroche

Book on SV-BBS


Technical Specifications

Imaging: Confocal & Widefield
Stand:
Inverted Axio Observer
XY-Stage: Motorized XYZ
Illumination: 100W Hal (transmission), Colibri 5 for (fluorescence)
Software: ZEN Blue ver. 3.4.91

Detectors

2 photomultiplier tubes (PMT) for fluorescence                                                  4 GaAsP detectors for fluorescence
2 high sensitivity near infrared (NIR) detector for fluorescence
1 Airyscan (32 channel PMT) for fluorescence
1 transmission photomultiplier tube (tPMT) for brightfield

CameraAxiocam 506 mono
Chip TechnologyCCD Grayscale
Sensor Format 2752 x 2208
Chip size12.5 mm x 10.0 mm
Pixel size4.54 um x 4.54 um
Dynamic Range14-bit
C-mount1.0x

Objectives

ObjectiveMag/NAMediumContrastWD (mm)
EC Plan-Neofluar10x/0.30air5.2
Plan-Apochromat20x/0.80airDIC0.55
LD LCI Plan Apochromat25x/0.80 CorrImmDIC0.57 at 0.17
LD LCI Plan Apochromat40x/1.20 autocorrImmDIC0.41 at 0.17
Plan-Apochromat63x/1.40oilDIC0.19

Laser Lines

WavelengthLaser Type
405 nmDiode
445 nmDiode
488 nmDiode
561nmDPSS
639 nmDiode
730 nmDiode

Filtercubes

The links in the table below will send you to an FPbase configuration page.

NameExcitationDichroicEmissionNo
BlueBP 390/40FT 420BP 450/40FS 96 HE
GreenBP 470/40FT 495BP 525/50FS 38 HE
RedBP 550/25FT 570BP 605/70FS 43 HE
Far-RedBP 640/30FT 660BP 690/50FS 50