Olympus FV 4000 IN 1

Leica SP8 UP1 Microscope
Flexible inverted confocal microscope with resonant scanner and motorized stage. Ideal for widefield and confocal tiled acquisitions.

Multipurpose point scanning confocal microscope with SilVIR detectors and CMOS camera. Prior ProScan III motorized stage.

Boasts full temperature and CO2 Control

Person in charge:

Book on SV-PPMS


Technical Specifications

Imaging: Confocal & Widefield
Stand:
Inverted IX 83
XY-Stage: Prior ProScan III motorized XY
Illumination: transmission: LED, FL: U-LPGS, 4 laser
Software: “cellSens” for widefield, “cellSens Fluoview” for confocal

Detectors

2 Spectral detectors (SilVir) for fluorescence 
1 Transmission photomultiplier tube (PMT) for brightfield

CameraHamamatsu Orca Flash4 LT3
Chip TechnologyCMOS Grayscale
Sensor Format 2048 x 2048
Chip size13.312 mm x 13.312 mm
Pixel size6.5 um x 6.5 um
Dynamic Range16 bit
C-mount1.0x

Objectives

ObjectiveMag/NAMediumContrastWD (mm)
UPLXAPO4x/0.16air13.0
UPLXAPO10x/0.40air3.10
UPLXAPO20x/0.80air0.60
UPLXAPO30x/1.05Sil0.80
UPLXAPO40x/1.40oilDIC0.13
UPLXAPO60x/1.42oil0.15

Laser Lines

WavelengthLaser Type
405 nmSolid state, Coherent
488 nmSolid state, Coherent
561 nmSolid state, Coherent
640 nmSolid state, Coherent

Filtercubes

The links in the table below will send you to an FPbase configuration page.

NameExcitationDichroicEmissionNo
DAPI350/50400LP460/50E0439584
GFP480/30505DC535/40E0439743
RHOD540/25565DC605/55E0439747
Y5620/50655DC690/50E0439749

Tutorial