Leica SP8 UP1

Leica SP8 UP1 Microscope
Flexible Upright confocal microscope with resonant scanner and motorized stage. Ideal for widefield and confocal tiled acquisitions.

Multipurpose point scanning confocal microscope with PMT, tPMT, HyD and CCD camera detectors. Motorized stage with Navigator for convenient tiling.

Person in charge: José Artacho & Thierry Laroche

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Technical Specifications

Imaging: Confocal & Widefield
Stand:
Upright Leica DM6 CS
XY-Stage: Motorized
Illumination: Lumencor Sola SM II LED, Laser
Software: LAS-X

Detectors

2 Hybrid Detectors (HyD) for fluorescence
1 photomultiplier tube (PMT) for fluorescence
1 transmission photomultiplier tube (PMT) for brightfield

CameraDFC 7000 GT (B/W)
Chip TechnologyCCD Grayscale
Sensor Format 1920 x 1440
Chip size8.8 mm x 6.6 mm
Pixel size4.54 um x 4.54 um
Dynamic Range8 – 12 bit
C-mount1.0x

Objectives

ObjectiveMag/NAMediumContrastWD (mm)
HC PL Fluotar5x/0.15airDIC POL13.7
HC PL Fluotar10x/0.30airDIC POL11.0
HC APO20x/0.5waterDIC POL3.5
HC PL APO20x/0.75airDIC POL0.62
HC PL APO40x/1.25glycDIC POL0.14
HC PL APO63x/1.40oilDIC POL0.14

Laser Lines

WavelengthLaser Type
405 nmSolid state
488 nmSolid state
552 nmSolid state
638 nmSolid state

Filtercubes

The links in the table below will send you to an FPbase configuration page.

NameExcitationDichroicEmissionNo
DAPIBP 350/50400BP 460/50DAPI ET
GFPBP 450-490495BP 500-550GFP ET
RHODBP 546/10560BP 585/40RHO ET
Y5BP 620/60660BP 700/75Y5 ET

Tutorial