Leica SP8 STED 3X

Leica SP8 STED 3X
BIOP Leica Stimulated Emisson Depletion Microscope.

Leica SP8 STED Microscope, with hybrid photon counting detectors (HyD). Capable of Stimulated Emission Depletion (STED) for 3D superresolution up to 40nm.

Boasts full tempterature and CO2 Control and a pulsed supercontinuum tunable white light laser.

Person in charge: José Artacho & Nicolas Chiaruttini

Book on SV-BBS


Technical Specifications

Stand: Inverted DMI6000 motorized
XY-Stage: Motorized XY
Illumination: HXP-120 Metal Halide
Software: LEICA LAS-X

STED Module

Depletion Lasers:

  • 592 nm
  • 660 nm
  • 775 nm (pulsed)

Detectors

Detector TypeAmount
PMTx3
HyDx2
Transmission PMTx1

Objectives

ObjectiveMag/NAMediumContrastWD (mm)
HC PL FLUOTAR10x/0.30air11.00
HC PL APO Corr20x/0.75IMM2.40
HC PL APO63x/1.40oil0.14
HC PL APO Corr93x/1.30+ glyc**
HC PL APO Corr100x/1.40+oil0.13

+ Used for STED imaging.
** Motorized correction ring
.

Laserlines

WavelenghtsLaser Type
405 nmDiode
Supercontinuum laserRange: 470 nm – 670 nm

Filtercubes

The links in the table below will send you to an FPbase configuration page.

NameExcitationDichroicEmissionNo
Blue350/50400460/5011525304
Green480/40505527/3011525307
Red540/45580590 LP11525303