Leica SP8 STED 3X

Leica SP8 STED 3X
BIOP Leica Stimulated Emisson Depletion Microscope.

Leica SP8 STED Microscope, with hybrid photon counting detectors (HyD). Capable of Stimulated Emission Depletion (STED) for 3D superresolution up to 40nm.

Boasts full tempterature and CO2 Control and a pulsed supercontinuum tunable white light laser.

Person in charge: José Artacho & Nicolas Chiaruttini

Book on SV-BBS


Technical Specifications

Stand: Inverted DMI6000 motorized
XY-Stage: Motorized XY
Illumination: HXP-120 Metal Halide
Software: LEICA LAS-X

STED Module

Depletion Lasers:

  • 592 nm
  • 660 nm
  • 775 nm (pulsed)

Detectors

Detector Type Amount
PMT x3
HyD x2
Transmission PMT x1

Objectives

Objective Mag/NA Medium Contrast WD (mm)
HC PL FLUOTAR 10x/0.30 air 11.00
HC PL APO Corr 20x/0.75 IMM 2.40
HC PL APO 63x/1.40 oil 0.14
HC PL APO Corr 93x/1.30+ glyc**
HC PL APO Corr 100x/1.40+ oil 0.13

+ Used for STED imaging.
** Motorized correction ring
.

Laserlines

Wavelenghts Laser Type
405 nm Diode
Supercontinuum laser Range: 470 nm – 670 nm

Filtercubes

The links in the table below will send you to an FPbase configuration page.

Name Excitation Dichroic Emission No
Blue 350/50 400 460/50 11525304
Green 480/40 505 527/30 11525307
Red 540/45 580 590 LP 11525303