Leica SP5 2-Photon

Leica SP5 2-Photon Microscope
2 Photon microscope for deep tissue imaging and second harmonics imaging. Can be used for ablation. Full temperature and CO2 control.

Leica SP5 2 Photon Microscope for deep imaging of live samples.

Boasts full tempterature and CO2 control and a tunable 2-photon laser.

Person in charge: Thierry Laroche

Book on SV-BBS


Technical Specifications

Stand: Upright DM 6000 CFS motorized
XY-Stage: Motorized
Illumination: HXP-120 Metal Halide
Software: Leica LAS-X

2-Photon Specifications

Chameleon Ultra Laser:

  • Pulse Width: < 140 fs at peak
  • Average Power: > 2.0 W
  • Repetition Rate: 80MHz
  • Tuning Range: 690 nm – 1080 nm

Detectors

Detector Type Amount
PMT x2
Non-Descanned Detector (NDD) x4
Transmission PMT x1

Objectives

Objective Mag/NA Medium Contrast WD (mm)
HC PL APO 10x/0.40 IMM 0.36
HC PL APO Corr 63x/1.20 corr water 0.22
HC PL APO 20x/1.00 water 2.00
HC PL APO Corr 20x/0.70 corr imm

Laserlines

Wavelenghts Laser Type
633 nm HeNe
2P Laser Range: 690 nm – 1080 nm

Filtercubes

Name Beamsplitter Barrier 1 Barrier 2 No
FITC / TRITC RSP 560 ET 520-50 HC 585-40 15 6504
DAPI / FITC RSP 495 HC 457/50 ET 525/40 15 6504 241
DAPI / TRITC RSP 495 HC 457/50 HC 585/40 15 6504 242
CFP / YFP RSP 505 HC 483/32 ET 535/30 15 6504 243
FITC / TXR RSP 560 ET 525/50 ET 617/73 156504 244

Filtercubes for Second Harmonic Imaging

Reference Transmission Band Blocking Band Lot No
F75-680 BP 350-650 680-1040 209350-209353
F39-427 BP 450-490 408-800 608337-508346
F39-445 BP 435-455 470-960 406367-506338
F37-525 BD 500-550 400-1100 109336

Filtercubes for Oculars

Name Excitation Dichroic Emission No
Blue BP 344-380 FT 400 LP 425 A
Green BP 450-490 FT 510 LP 515 I3
Red BP 515-560 FT 580 LP 590 N2.1