Multipurpose point scanning confocal microscope with SilVIR detectors and CMOS camera. Prior ProScan III motorized stage.
Person in charge:
Book on SV-PPMS
Technical Specifications
Imaging: Confocal & Widefield
Stand: Inverted IX 83
XY-Stage: Prior ProScan III motorized XY
Illumination: transmission: LED, FL: U-LPGS, 4 laser
Software: “cellSens” for widefield, “cellSens Fluoview” for confocal
Detectors
2 Spectral detectors (SilVir) for fluorescence
1 Transmission photomultiplier tube (PMT) for brightfield
| Camera | Hamamatsu Orca Flash4 LT3 |
| Chip Technology | CMOS Grayscale |
| Sensor Format | 2048 x 2048 |
| Chip size | 13.312 mm x 13.312 mm |
| Pixel size | 6.5 um x 6.5 um |
| Dynamic Range | 16 bit |
| C-mount | 1.0x |
Objectives
| Objective | Mag/NA | Medium | Contrast | WD (mm) |
| UPLXAPO | 4x/0.16 | air | 13.0 | |
| UPLXAPO | 10x/0.40 | air | 3.10 | |
| UPLXAPO | 20x/0.80 | air | 0.60 | |
| UPLXAPO | 30x/1.05 | Sil | 0.80 | |
| UPLXAPO | 40x/1.40 | oil | DIC | 0.13 |
| UPLXAPO | 60x/1.42 | oil | 0.15 |
Laser Lines
| Wavelength | Laser Type |
| 405 nm | Solid state, Coherent |
| 488 nm | Solid state, Coherent |
| 561 nm | Solid state, Coherent |
| 640 nm | Solid state, Coherent |
Filtercubes
The links in the table below will send you to an FPbase configuration page.
Tutorial
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