The GECF provides access to 3 QuantStudio 6/7 qPCR instruments.
In addition, 384-w plates assembly can be performed on our automated Hamilton liquid handling platform.
Training
In order to use the Hamilton liquid handling platform, you have to be trained. Training is performed with dummy samples provided by the GECF. Please contact us to arrange a schedule, and please read our user manual in advance.
This Excel file will guide you for determining how to prepare the different mixes according to the number of genes/assays and samples/templates. These excel list of genes and templates are also required.
To use our QuantStudio 6/7 instruments, follow this procedure.
Booking is then performed through PPMS.
Costs
The cost for the use of the Hamilton robot is 35.25 CHF /30 minutes. All plasticware is included. Half an hour is enough to prepare a full 384-wells plate.
qPCR data analysis
Our user guide contains a list of software useful for advanced analysis (selection of the optimal normalizer gene, interplate calibration etc).