10x Genomics Chromium Single Cell Controller

CAUTION: At least 3 weeks prior notice is needed for a regular scRNA-seq experiment, but more may be needed for more complex experiments (multiome, multiplexing, custom protocols…), and when performing a 10XG experiment for the first time. In any case, do not launch an experiment that requires samples collection on a specific date/week before having scheduled it with us.

Our 10X Genomics Chromium X instrument allows to address diverse biological questions by generating transcriptomics and/or chromatin accessibility data for thousands of single cells. You can either bring us cells and we’ll perform the experiment for you, or you can rent the instrument and perform everything on your side (with your reagents). Our price list is available here.

Classical methods

A submission form for 10X Genomics experiments is available here. Fill it as well as possible and send it to us before the experiment.

Samples submission guidelines for classical 3′ scRNA-seq are available here.

Details regarding the 3′ scRNA-seq multiplexing solution CellPlex are available here. And a sandbox calculation file is available here.

Samples submission guidelines for multiome (scATAC+scRNA-seq) are available here. An introduction is available here.

Please contact us for more details regarding these guidelines, or for more information regarding scATAC-seqscRNA-seq+VDJ immune profiling,  or co-quantification of cell surface proteins.

FLEX methods (aka Fixed RNA Profiling)

FLEX allows to fix cells and store them before processing, specially useful for samples obtained late in the evening or during weekends. Quality is as good as classical 3’GE method, for cheaper, but is only available fo mouse and human samples, and is not yet compatible with ATAC/multiome/VDJ. A submission form for 10XG FLEX experiments is available here. Fill it as well as possible and send it to us before the experiment.

Samples submission guidelines for FLEX are available here.

New: FLEX is now also compatible with concomitant protein quantification through oligo-tagged antibodies. Inquire for more details.

For Spatial Transcriptomics, please refer to our dedicated page.