A 10X Genomics Chromium X instrument allows the GECF to address diverse biological questions by generating transcriptomics and/or chromatin accessibility data for thousands of single cells. You can either bring us cells and we’ll perform the experiment for you, or you can rent the instrument and perform everything on your side (with your reagents). Our price list is available here.
Classical (non-FLEX) methods
A submission form for 10X Genomics experiments is available here. Fill it as well as possible and send it to us before the experiment.
Samples submission guidelines for the classical 3′ scRNA-seq are available here.
An addendum regarding 5′ scRNA-seq +VDJ is available here.
For multiplexing:
- Details regarding the totalseq antibodies-based method for 3′ and 5′ scRNA-seq multiplexing will be available here asap. A sandbox calculation file is available here. This multiplexing is compatible with both 3’GE v4 and 5’GE v3.
- 10XG just released a new “on-chip multiplexing” (OCM) solution for 3’GE and 5’GE, which does not necessitate any labelling/tagging. An addendum on OCM is available here.
Samples submission guidelines for multiome (scATAC+scRNA-seq) are available here. An introduction is available here. Standalone scATAC-seq is also possible, guidelines are here.
Please contact us for more details regarding these guidelines, or for more information regarding other 10X Genomics methods, e.g. CITE-seq (co-quantification of cell surface proteins) or CRISPR screens.
FLEX methods
FLEX allows to fix cells and store them before processing, specially useful for samples obtained late in the evening or during weekends. Quality is as good as classical 3’GE method, for cheaper, but is only available fo mouse and human samples, and is not yet compatible with ATAC/multiome/VDJ. A submission form for 10XG FLEX experiments is available here. Fill it as well as possible and send it to us before the experiment.
Samples submission guidelines for FLEX are available here.
FLEX is now also compatible with concomitant protein quantification through oligo-tagged antibodies. Inquire for more details.