Gene Expression Core Facility

DNA molecules (Adobe education license)

Mission

We provide a diverse set of tools for the functional and quantitative analysis of genomes and transcriptomes, both at single cells level or on bulk cell populations. This comprises in particular next-generation sequencing, classical or high-throughput microfluidics-based qPCR technologies and spatial transcriptomics analyses. In addition, we enable manipulation of the cellular gene expression program by providing different genome-wide collections of ORFs. Because new technologies constantly emerge, we are eager to discuss means to further improve and develop our toolbox.

Expertise

Next generation sequencing

Illumina short reads (MiSeq, NextSeq, NovaSeq), Oxford Nanopore long reads (MinION, Flongle)

Single cells genomics/transcriptomics

10X Genomics Chromium, Fluidigm C1…

Spatial Transcriptomics

10X Genomics Visium

Quantitative PCR

Classical (Applied Biosystems QuantStudio) or microfluidics-based (Fluidigm BioMark) qPCR

Nucleic acids QC

Profiling (TapeStation 4200 and Fragment Analyzer) and quantification (Qubit, Nanodrop One)

ORF & cDNA Collections

We manage and distribute several large collections of ORFs

News

 

May 2022

April 2022

  • We’ re starting organizing 10X Genomics scRNA-seq trainings more regularly, tentatively each 2 months. Getting training on this technology is currently the only way to make sure you will be able to run your samples on a given date, as we cannot accomodate all demands. Please inquire.

February 2022

  • New versions of our libraries submission form/guidelines are available here.

January 2022

  • We have launched our NovaSeq 6000 service! Please inquire.
  • A warm welcome to our new collaborator Pierrick Moreau.

October 2021

  • We have launched a Visium FFPE spatial transcriptomics service, in collaboration with HCF.
  • A NovaSeq 6000 will soon replace our HiSeq 4000! More news in December.

September 2021

April 2021

  • The replacement process for our HiSeq 4000 sequencer has begun, more news in 2 months.
  • Our new 10X Genomics single cell multiome (scATAC-seq+scRNA-seq) and CellPlex services are operational.
  • Our new QuantStudio 6 instrument is now operational.
  • Our new web tool CloneMap for ORFs browsing and QC is operational (thanks Fabrice David).

March 2021

2020

2019

2018

  • Our new HiSeq 4000 sequencer just arrived! Service will begin later in January.
  • We released libraries submission guidelines for NextSeq sequencing. They are available here.
  • We released RNA submission guidelines for RNA-seq, and gDNA submission guidelines for whole genome sequencing or exome sequencing. They are available here.
  • A slightly updated version of our price list is available here.
  • Our new 10X Genomics Chromium instrument is operational. This allows scaling up single cells transcriptomics experiments to thousands of cells.
  • New user-friendly short protocols are available for the TapeStation 4200.

2017

2016

  • Initial release of our new MOUSAIC collection of murine ORFs.
  • New improved versions of the Fragment Analyzer kits were released.
  • Our new Agilent TapeStation 4200 has arrived. This RNA/DNA profiling instrument nicely complements our Fragment Analyzer. Please contact us for more details.

2015