Super-resolution optical fluctuation imaging (SOFI) achieves resolution beyond the diffraction limit by computing higher order statistics (cumulants) of time series of stochastically blinking fluorophores. In contrast to the known localization concepts as PALM or STORM, the resolution enhancement in SOFI is the result of spatio-temporal cross cumulants calculated over the entire image sequence. SOFI is compatible with a wide range of blinking conditions and even for high labeling densities.
More details are given in our publications (see below).
- Geissbuehler, Stefan, et al. “Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI).” Optical Nanoscopy 1.1 (2012): 1-7.
- Geissbuehler, Stefan, et al. “Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging.” Nature communications 5.1 (2014): 1-7.
- Grußmayer, Kristin S., et al. “Spectral cross-cumulants for multicolor super-resolved SOFI imaging.” Nature communications 11.1 (2020): 1-8.
- Grußmayer, Kristin, et al. “Self-Blinking Dyes Unlock High-Order and Multiplane Super-Resolution Optical Fluctuation Imaging.” ACS nano 14.7 (2020): 9156-9165.