SOFI Super-resolution Imaging

SOFI Principle

   Super-resolution optical fluctuation imaging (SOFI) achieves resolution beyond the diffraction limit by computing higher order statistics (cumulants) of time series of stochastically blinking fluorophores. In contrast to the known localization concepts as PALM or STORM, the resolution enhancement in SOFI is the result of spatio-temporal cross cumulants calculated over the entire image sequence. SOFI is compatible with a wide range of blinking conditions and even for high labeling densities.

More details are given in our publications (see below).



SOFI Publications


  1. S. Geissbuehler, N. L. Bocchio, C. Dellagiacoma, C. Berclaz, M. Leutenegger, T. Lasser
    Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)
    Opt. Nanoscopy 1:4 (2012)
  2. S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. G. van der Goot, E. A. Dubikovskaya, T. Lasser, M. Leutenegger
    Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging
    Nature Commun. 5: 5830 (2014)
  3. S. Geissbuehler, C. Dellagiacoma, T. Lasser
    Comparison between SOFI and STORM
    Biomed. Opt. Express 2, 408-420 (2011)
  4. Vandenberg, W., Leutenegger, M., Lasser, T., Hofkens, J., & Dedecker, P. (2015).
    Diffraction-unlimited imaging: from pretty pictures to hard numbers. 
    Cell and tissue research360(1), 151-178.

SOFI Tutorial (coming soon)