Bachelor and Master projects

Available throughout 2022 – contact Pierre Gönczy for further information

Opening for up to 2 Masters students in the Gönczy Laboratory in 2022!

The project is to be chosen amongst the ones listed below. Each project is conducted ideally during 2 semesters, but can be adapted to last only 1. Contact Pierre Gönczy ([email protected]) to find out more!

1. Re-engineering SAS-6 proteins

Keywords: cell biology, centriole organelle, CRISPR/Cas9, microscopy

Objective: test whether centrioles can form and function in human cells with engineered SAS-6 proteins with altered sizes and/or symmetries.

Approaches: human cell culture, CRISPR/Cas9-mediated engineering, expansion microscopy, super-resolution microscopy.

Ideal for students in: Life Sciences, Bioengineering.

Wet

2. Analyzing centriole fate during zebrafish muscle formation

Keywords: cell and developmental biology, muscle formation, zebrafish embryos, centrosome and centriole, live imaging

Objective: monitor centrosome and centriole fate during muscle formation in zebrafish embryos

Approaches: injection of RNA/DNA into zebrafish embryos, 4D live imaging using light-sheet microscopy, develop and apply tracking algorithms to monitor centrosomes and centrioles.

Ideal for students in: Life Sciences, Bioengineering.

Wet

 3. Centriole inheritance in sexual and asexual reproduction

Keywords: cell and developmental biology, embryos, parthenogenesis, centriole, live imaging

Objective: investigate how centrioles are inherited/formed in embryos generated through asexual reproduction, where oocytes develop without fertilization by sperm.

Approaches: live imaging of early embryogenesis in the nematode Panagrolaimus, identification of homologues of C. elegans centriolar proteins, protein expression and purification, antibody generation, immunofluorescence analysis, confocal imaging.

Ideal for students in: Life Sciences, Bioengineering.

Wet

4. Analyzing novel centriolar proteins in Chlamydomonas reinhardtii

Keywords: cell biology, green algae, centriole, CRISPR/Cas9, microscopy

Objective: identify the localization and test the function of novel centriolar proteins in the green alga Chlamydomonas reinhardtii.

Approaches: CRISPR/Cas9-mediated GFP tagging, as well as disruption, of novel centriolar proteins in Chlamydomonas reinhardtii, expansion microscopy, super-resolution microscopy.

Ideal for students in: Life Sciences, Bioengineering.

Wet

5. Analyzing genes that set organismal thermal limits

Keywords: yeast, worms, thermal range, computational biology, functional genomics

Objective: analyze outcome of a screen performed in the yeast S. cerevisiae to identify genes important for determining organismal thermal range; investigate whether their function is conserved in the nematode C. elegans.

Approaches: computational biology, functional genomics, microscopy.

Ideal for students in: Life Sciences, Computer Sciences

Dry + Wet

6. Analyzing flagellum formation in a new model organism for multicellularity

Keywords: protist, cell-cycle, centriole, flagellum, microscopy

Objective: characterize the life cycle of the Ichthyosporean Chromosphaera perkinsii, identifying when and where flagellated cells appear; describe flagellar organization and analyse centrioles using immunofluorescence and electron microscopy.

Approaches: conduct live cell imaging of C. perkinsii in different growth conditions, establish synchronization protocol using cell sorting, perform immunofluorescence analysis, as well as transmission and scanning electron microscopy.

Ideal for students in: Life Sciences

Supervised by Omaya Dudin (Ambizione Fellow)

Wet