Stem Cell and Organoid Laboratory (SCOL)

We provide access to fully equipped P1 and P2 cell culture laboratories and support for the development of organoid and iPSC models including:

• Teaching basic organoid and iPSC culture methods to researchers

• Centralising the purchase and distribution of common culture reagents

• Biobanking of organoids and iPSCs (storage and repository)

Resources
  • Fully equipped P1 and P2 cell culture rooms
  • Dissection and quarantine room
  • Media, basal membrane extracts and culture supplements
  • Electroporator (Nepagene NEPA21)
  • Cryofreezer (Kryo 560M-16M)
  • Mouse organoid repository
How to get access?
Services
  • Portfolio of protocols
  • Support during establishment of new protocols
  • Cryopreservation
  • Mycoplasma testing
Training
  • Human and mouse organoid maintenance (feeding and passaging)
  • Human and mouse organoid cryopreservation (freezing and thawing)
  • Fixation and whole mount IF staining of organoids
  • DNA/RNA/protein isolation from organoids
  • Electroporation
Coming soon
  • Organoid gene modification
  • Derivation of mouse organoids 
  • Human/mouse iPSC and organoid repository
  • iPSC maintenance and differentiation