We provide access to fully equipped P1 and P2 cell culture laboratories and support for the development of organoid and iPSC models including:
• Teaching basic organoid and iPSC culture methods to researchers
• Centralising the purchase and distribution of common culture reagents
• Repository of organoids and iPSCs
Resources
- Fully equipped P1 and P2 cell culture rooms
- Dissection and quarantine room
- Mouse organoid repository
How to get access?
- Access request
- Discussion of the planned project and its execution
- Acceptance of the rules
- Training
- Access to reservation system
Services
- Portfolio of protocols
- Support during establishment of new protocols
-
Mouse organoids (cryopreserved and derivation)
- Cryopreservation
-
Mycoplasma testing
Training
- Human and mouse organoid maintenance
- Human and mouse organoid cryopreservation
-
Derivation of mouse organoids
-
Organoid gene modification
- Fixation and whole mount IF staining of organoids
- Electroporation
Equipments
- Electroporator (Nepagene NEPA21)
- Cryofreezer (Kryo 560M-16M)
- Life-cell imaging and analysis (Incucyte S3, Sartorius)
- Cell imaging multimode reader (Cytation5, BioTek)
- Bioreactor (Bio-Levitator, Hamilton)