The true shape of dendritic spines – December 2020

Using cryo fixation, the BioEM facility shows how dendritic spines are altered by chemical fixation. The dendritic spine is the site where the majority of  excitatory connections are found in the adult brain. There size and shape are important determinants of their function.  A detailed morphological analysis now published in eLife shows few changes, however, cryo-fixed spines have significantly thinner necks.

TEM in Parkinson’s disease

The group of Hilal Lashuel publishes an extensive study in the journal PNAS in which the progression of Lewy body formation is tracked in cells. This includes transmission electron microscopy with immunocytochemistry. See the EPFL news here. The research article can be viewed here.

Block face scanning EM in Alzheimer’s Disease

A study lead by Gilles Bonvento (Paris) explores astrocytic metabolism and mechanistic link that connects defective glycolysis in astrocytes to impaired l-serine production and reduced neuronal function. Supplementation with L-serine in a mouse model of Alzheimer’s restores cognitive deficits. This study used the block-face scanning electron microscopy services offered by the BioEM Facility. The study is published in the journal Cell Metabolism, and can be viewed here.


Mechanopathology of biofilm-like Mycobacterium tuberculosis cords

R. Mishra; M. T. Hannebelle; V. P. Patil; A. Dubois; C. Garcia-Mouton et al. 

Cell. 2023-11-09. Vol. 186, num. 23, p. 5135-+. DOI : 10.1016/j.cell.2023.09.016.

Stable isotope labeling and ultra-high-resolution NanoSIMS imaging reveal alpha-synuclein-induced changes in neuronal metabolism in vivo

S. Spataro; B. Maco; S. Escrig; L. Jensen; L. Polerecky et al. 

Acta Neuropathologica Communications. 2023-09-29. Vol. 11, num. 1, p. 157. DOI : 10.1186/s40478-023-01608-8.

Dynamics of CLIMP-63 S-acylation control ER morphology

P. A. Sandoz; R. A. Denhardt-Eriksson; L. Abrami; L. A. Abriata; G. Spreemann et al. 

Nature Communications. 2023-01-17. Vol. 14, num. 1. DOI : 10.1038/s41467-023-35921-6.

Large-scale morphometry of the subarachnoid space of the optic nerve

D. Rossinelli; H. E. Killer; P. Meyer; G. Knott; G. Fourestey et al. 

Fluids And Barriers Of The Cns. 2023-03-21. Vol. 20, num. 1, p. 21. DOI : 10.1186/s12987-023-00423-6.

Correlative light and volume electron microscopy to study brain development

S. Hayashi; N. Ohno; G. Knott; Z. Molnar 

Microscopy. 2023-01-09. DOI : 10.1093/jmicro/dfad002.


Retromer deficiency in Tauopathy models enhances the truncation and toxicity of Tau

J. Asadzadeh; E. Ruchti; W. Jiao; G. Limoni; C. MacLachlan et al. 

Nature Communications. 2022-08-27. Vol. 13, num. 1, p. 5049. DOI : 10.1038/s41467-022-32683-5.

ADAMTS18(+) villus tip telocytes maintain a polarized VEGFA signaling domain and fenestrations in nutrient-absorbing intestinal blood vessels

J. Bernier-Latmani; C. Mauri; R. Marcone; F. Renevey; S. Durot et al. 

Nature Communications. 2022-07-09. Vol. 13, num. 1, p. 3983. DOI : 10.1038/s41467-022-31571-2.

A subpopulation of cortical VIP-expressing interneurons with highly dynamic spines

C. Georgiou; V. Kehayas; K. S. Lee; F. Brandalise; D. A. Sahlender et al. 

Communications Biology. 2022-04-13. Vol. 5, num. 1, p. 352. DOI : 10.1038/s42003-022-03278-z.


Toward Biophysical Mechanisms of Neocortical Computation after 50 Years of Barrel Cortex Research

C. C. H. Petersen; G. W. Knott; A. Holtmaat; F. Schuermann 

Function. 2021-01-01. Vol. 2, num. 1, p. zqaa046. DOI : 10.1093/function/zqaa046.

Nuclear and cytoplasmic huntingtin inclusions exhibit distinct biochemical composition, interactome and ultrastructural properties

N. Riguet; A-L. Mahul-Mellier; N. Maharjan; J. Burtscher; M. Croisier et al. 

Nature Communications. 2021-11-12. Vol. 12, num. 1, p. 6579. DOI : 10.1038/s41467-021-26684-z.

Early invasion of the bladder wall by solitary bacteria protects UPEC from antibiotics and neutrophil swarms in an organoid model

K. Sharma; V. V. Thacker; N. Dhar; M. Clapés Cabrer; A. Dubois et al. 

Cell Reports. 2021-07-20. Vol. 36, num. 3, p. 109351. DOI : 10.1016/j.celrep.2021.109351.

Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection

K. Sharma; N. Dhar; V. V. Thacker; T. M. Simonet; F. Signorino-Gelo et al. 

eLife. 2021-07-05. Vol. 10, p. e66481. DOI : 10.7554/eLife.66481.

Deep Active Surface Models

P. U. Wickramasinghe; P. Fua; G. Knott 

2021-06-24. Conference on Computer Vision and Pattern Recognition (CVPR), Virtual, June 21-25, 2021. p. 11652-11661. DOI : 10.1109/CVPR46437.2021.01148.

Maturation of Complex Synaptic Connections of Layer 5 Cortical Axons in the Posterior Thalamic Nucleus Requires SNAP25

S. Hayashi; A. Hoerder-Suabedissen; E. Kiyokage; C. Maclachlan; K. Toida et al. 

Cerebral Cortex. 2021-05-01. Vol. 31, num. 5, p. 2625-2638. DOI : 10.1093/cercor/bhaa379.

3D Ultrastructure of Synaptic Inputs to Distinct GABAergic Neurons in the Mouse Primary Visual Cortex

Y-S. Hwang; C. Maclachlan; J. Blanc; A. Dubois; C. C. H. Petersen et al. 

Cerebral Cortex. 2021-05-01. Vol. 31, num. 5, p. 2610-2624. DOI : 10.1093/cercor/bhaa378.

Mitofusin-2 in nucleus accumbens regulates anxiety and depression-like behaviors through mitochondrial and neuronal actions

E. Gebara; O. Zanoletti; S. Ghosal; J. Grosse; B. L. Schneider et al. 

Biological Psychiatry. 2021. Vol. 89, num. 11, p. 1024-1026. DOI : 10.1016/j.biopsych.2020.12.003.


Ultrastructural comparison of dendritic spine morphology preserved with cryo and chemical fixation

H. Tamada; J. Blanc; N. Korogod; C. C. Petersen; G. W. Knott 

Elife. 2020-12-04. Vol. 9, p. e56384. DOI : 10.7554/eLife.56384.

Nano-imaging trace elements at organelle levels in substantia nigra overexpressing alpha-synuclein to model Parkinson’s disease

L. Lemelle; A. Simionovici; P. Colin; G. Knott; S. Bohic et al. 

Communications Biology. 2020-07-09. Vol. 3, num. 1, p. 364. DOI : 10.1038/s42003-020-1084-0.

Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth

C. Contat; P-B. Ancey; N. Zangger; S. Sabatino; J. Pascual et al. 

Elife. 2020-06-23. Vol. 9, p. e53618. DOI : 10.7554/eLife.53618.

Voxel2Mesh: 3D Mesh Model Generation from Volumetric Data

P. U. Wickramasinghe; E. Remelli; G. Knott; P. Fua 

2020. International Conference On Medical Image Computing & Computer Assisted Intervention (MICCAI), Lima, Peru, 4-8 OCTOBER 2020.

Somatostatin enhances visual processing and perception by suppressing excitatory inputs to parvalbumin-positive interneurons in V1

Y-H. Song; Y-S. Hwang; K. Kim; H-R. Lee; J-H. Kim et al. 

Science Advances. 2020-04-01. Vol. 6, num. 17, p. eaaz0517. DOI : 10.1126/sciadv.aaz0517.

Dysfunction of homeostatic control of dopamine by astrocytes in the developing prefrontal cortex leads to cognitive impairments

F. Petrelli; G. Dallerac; L. Pucci; C. Cali; T. Zehnder et al. 

Molecular Psychiatry. 2020-04-01. Vol. 25, num. 4, p. 732-749. DOI : 10.1038/s41380-018-0226-y.

Impairment of Glycolysis-Derived L-Serine Production in Astrocytes Contributes to Cognitive Deficits in Alzheimer’s Disease

J. Le Douce; M. Maugard; J. Veran; M. Matos; P. Jego et al. 

Cell Metabolism. 2020-03-03. Vol. 31, num. 3, p. 503-517.e8. DOI : 10.1016/j.cmet.2020.02.004.

Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolution

K. J. Hayworth; D. Peale; M. Januszewski; G. W. Knott; Z. Lu et al. 

Nature Methods. 2020-11-18. Vol. 17, num. 1, p. 68-71. DOI : 10.1038/s41592-019-0641-2.

The process of Lewy body formation, rather than simply α-synuclein fibrillization, is one of the major drivers of neurodegeneration

A-L. Mahul-Mellier; J. Burtscher; N. Maharjan; L. Weerens; M. Croisier et al. 

Proceedings of the National Academy of Sciences of United States of America. 2020-02-19.  p. 201913904. DOI : 10.1073/pnas.1913904117.

Amygdala GluN2B-NMDAR dysfunction is critical in abnormal aggression of neurodevelopmental origin induced by St8sia2 deficiency

A. Bacq; S. Astori; E. Gebara; W. Tang; B. A. Silva et al. 

Mol Psychiatry. 2020. Vol. 25, num. 9, p. 2144-61. DOI : 10.1038/s41380-018-0132-3.


Probabilistic Atlases to Enforce Topological Constraints

P. U. Wickramasinghe; G. Knott; P. Fua 

2019-10-10. 22rd International Conference On Medical Image Computing & Computer Assisted Intervention, Shenzhen, China, 2019-10-13. DOI : 10.1007/978-3-030-32239-7_25.

Synaptic proximity enables NMDAR signalling to promote brain metastasis

Q. Zeng; I. P. Michael; P. Zhang; S. Saghafinia; G. Knott et al. 

Nature. 2019-09-26. Vol. 573, num. 7775, p. 526-531. DOI : 10.1038/s41586-019-1576-6.

Diversity of Cortico-descending Projections: Histological and Diffusion MRI Characterization in the Monkey

G. M. Innocenti; R. Caminiti; E. M. Rouiller; G. Knott; T. B. Dyrby et al. 

Cerebral Cortex. 2019-02-01. Vol. 29, num. 2, p. 788-801. DOI : 10.1093/cercor/bhx363.

The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging

D. D’Amico; A. Mottis; F. Potenza; V. Sorrentino; H. Li et al. 

Molecular Cell. 2019-02-21. Vol. 73, num. 4, p. 775-787.e10. DOI : 10.1016/j.molcel.2018.11.034.

Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

C. Battistella; R. Guiet; O. Burri; A. Seitz; S. Escrig et al. 

Biomacromolecules. 2019. Vol. 20, num. 1, p. 231-242. DOI : 10.1021/acs.biomac.8b01372.


NeuroMorph: A Software Toolset for 3D Analysis of Neurite Morphology and Connectivity

A. Jorstad; J. Blanc; G. Knott 

Frontiers in Neuroanatomy. 2018. Vol. 12, p. 59. DOI : 10.3389/fnana.2018.00059.

Ultrastructural basis of strong unitary inhibition in a binaural neuron

E. Gjoni; C. Aguet; D. A. Sahlender; G. Knott; R. Schneggenburger 

Journal Of Physiology-London. 2018-10-15. Vol. 596, num. 20, p. 4969-4982. DOI : 10.1113/JP276015.

In vivo modeling of human neuron dynamics and Down syndrome

R. Real; M. Peter; A. Trabalza; S. Khan; M. A. Smith et al. 

Science. 2018-11-16. Vol. 362, num. 6416, p. eaau1810. DOI : 10.1126/science.aau1810.

Locally coordinated synaptic plasticity of visual cortex neurons in vivo

S. El-Boustani; J. P. K. Ip; V. Breton-Provencher; G. W. Knott; H. Okuno et al. 

Science. 2018-06-22. Vol. 360, num. 6395, p. 1349-1354. DOI : 10.1126/science.aao0862.

Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding

C. Maclachlan; D. A. Sahlender; S. Hayashi; Z. Molnar; G. Knott 

Frontiers In Neuroanatomy. 2018-11-06. Vol. 12, p. 88. DOI : 10.3389/fnana.2018.00088.

The effects of aging on neuropil structure in mouse somatosensory cortex-A 3D electron microscopy analysis of layer 1

C. Cali; M. Wawrzyniak; C. Becker; B. Maco; M. Cantoni et al. 

PLOS ONE. 2018. Vol. 13, num. 7, p. e0198131. DOI : 10.1371/journal.pone.0198131.

Neuron Segmentation With High-Level Biological Priors

N. Krasowski; T. Beier; G. Knott; U. Kothe; F. Hamprecht et al. 

IEEE TRANSACTIONS ON MEDICAL IMAGING. 2018. Vol. 37, num. 4, p. 829-839. DOI : 10.1109/TMI.2017.2712360.

Molecular insights into Vibrio cholerae’s intra-amoebal host-pathogen interactions

C. Van der Henst; A. S. Vanhove; N. C. Drebes Dörr; S. Stutzmann; C. Stoudmann et al. 

Nature Communications. 2018. Vol. 9, p. 3460. DOI : 10.1038/s41467-018-05976-x.


Computer assisted detection of axonal bouton structural plasticity in in vivo time-lapse images

R. Gala; D. Lebrecht; D. A. Sahlender; A. Jorstad; G. Knott et al. 

Elife. 2017. Vol. 6, p. e29315. DOI : 10.7554/eLife.29315.

The Differential Distribution of RAPTA-T in Non-Invasive and Invasive Breast Cancer Cells Correlates with Its Anti-Invasive and Anti-Metastatic Effects

R. F. S. Lee; S. Escrig; C. Maclachlan; G. W. Knott; A. Meibom et al. 

International Journal Of Molecular Sciences. 2017. Vol. 18, num. 9, p. 1869. DOI : 10.3390/ijms18091869.

Homeostatic control of dopamine by astrocytes in the postnatal maturation of the prefrontal cortex

P. Bezzi; F. Petrelli; L. Pucci; G. Dallerac; C. Cali et al. 

2017. ISN-ESN Meeting, Paris, FRANCE, AUG 20-24, 2017. p. 18-18.

Identification of aminopyrimidine-sulfonamides as potent modulators of Wag31-mediated cell elongation in mycobacteria

V. Singh; N. Dhar; J. Pato; G. S. Kolly; J. Kordulakova et al. 

Molecular Microbiology. 2017. Vol. 103, num. 1, p. 13-25. DOI : 10.1111/mmi.13535.

Parkin functionally interacts with PGC-1 proportional to to preserve mitochondria and protect dopaminergic neurons

L. Zheng; N. Bernard-Marissal; N. Moullan; D. D’Amico; J. Auwerx et al. 

Human Molecular Genetics. 2017. Vol. 26, num. 3, p. 582-598. DOI : 10.1093/hmg/ddw418.

Multicut brings automated neurite segmenation closer to human performance

T. Beier; C. Pape; N. Rahaman; L. Prange; S. Berg et al. 

Nature Methods. 2017. Vol. 14, num. 2, p. 101-102. DOI : 10.1038/nmeth.4151.


Cell Division by Longitudinal Scission in the Insect Endosymbiont

E. Ramond; C. Maclachlan; S. Clerc-Rosset; G. W. Knott; B. Lemaitre 

mBio. 2016. Vol. 7, num. 4, p. e00881-16. DOI : 10.1128/mBio.00881-16.

Multi-Modal Optical Imaging of the Cerebellum in Animals

A. L. A. Mascaro; L. Sacconi; L. Silvestri; G. Knott; F. S. Pavone 

2016. 7th International Symposium of the Society-for-Research-on-the-Cerebellum (SRC), Brussels, BELGIUM, MAY 08-10, 2015. p. 18-20. DOI : 10.1007/s12311-015-0730-4.


C6-O-03Quantifying adult brain ultrastructure using focussed ion beam scanning electron microscopy

G. Knott 

Microscopy. 2015. Vol. 64, p. i74-i74. DOI : 10.1093/jmicro/dfv203.

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

R. F. S. Lee; S. Escrig; M. Croisier; S. Clerc-Rosset; G. Knott et al. 

Chemical Communications. 2015. Vol. 51, num. 92, p. 16577-16577. DOI : 10.1039/c5cc90487k.

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

R. F. S. Lee; S. Escrig; M. Croisier; S. Clerc-Rosset; G. W. Knott et al. 

Chemical Communications. 2015. Vol. 51, num. 92, p. 16486-16489. DOI : 10.1039/c5cc06983a.

PGC-1 alpha activity in nigral dopamine neurons determines vulnerability to alpha-synuclein

C. Ciron; L. Zheng; W. Bobela; G. W. Knott; T. C. Leone et al. 

Acta Neuropathologica Communications. 2015. Vol. 3, p. 16. DOI : 10.1186/s40478-015-0200-8.

Imaging the time-integrated cerebral metabolic activity with subcellular resolution through nanometer-scale detection of biosynthetic products deriving from (13)C-glucose

Y. Takado; G. Knott; B. M. Humbel; M. Masoodi; S. Escrig et al. 

Journal of chemical neuroanatomy. 2015. Vol. 69, p. 7-12. DOI : 10.1016/j.jchemneu.2015.09.003.

Delayed and Temporally Imprecise Neurotransmission in Reorganizing Cortical Microcircuits

S. J. Barnes; C. E. Cheetham; Y. Liu; S. H. Bennett; G. Albieri et al. 

Journal Of Neuroscience. 2015. Vol. 35, num. 24, p. 9024-9037. DOI : 10.1523/Jneurosci.4583-14.2015.

Ultrastructural analysis of adult mouse neocortex comparing aldehyde perfusion with cryo fixation

N. Korogod; C. C. H. Petersen; G. W. Knott 

Elife. 2015. Vol. 4, p. e05793. DOI : 10.7554/eLife.05793.

A single epidermal stem cell strategy for safe ex vivo gene therapy

S. Droz-Georget Lathion; A. Rochat; G. Knott; A. Recchia; D. Martinet et al. 

EMBO molecular medicine. 2015. Vol. 7, num. 4, p. 380-93. DOI : 10.15252/emmm.201404353.

Multiphoton microscopy in brain imaging

A. L. A. Mascaro; L. Silvestri; I. Costantini; L. Sacconi; B. Maco et al. 

2015. Conference on Multiphoton Microscopy in the Biomedical Sciences XV, San Francisco, CA, FEB 08-10, 2015. DOI : 10.1117/12.2084183.

Cold shock protects the brain

G. Knott 

Nature. 2015. Vol. 518, num. 7538, p. 177-178. DOI : 10.1038/nature14195.

Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomics

K. J. Hayworth; C. S. Xu; Z. Lu; G. W. Knott; R. D. Fetter et al. 

Nature Methods. 2015. Vol. 12, num. 4, p. 319-U55. DOI : 10.1038/Nmeth.3292.

Modeling Brain Circuitry over a Wide Range of Scales

P. Fua; G. Knott 

Frontiers in Neuroanatomy. 2015. Vol. 9, p. 42. DOI : 10.3389/fnana.2015.00042.

Learning Structured Models for Segmentation of 2D and 3D Imagery

A. Lucchi; P. Márquez-Neila; C. Becker; Y. Li; K. Smith et al. 

IEEE Transactions on Medical Imaging. 2015. Vol. 34, num. 5, p. 1096-1110. DOI : 10.1109/TMI.2014.2376274.

Imaging liver and brain glycogen metabolism at the nanometer scale

Y. Takado; G. Knott; B. M. Humbel; S. Escrig; M. Masoodi et al. 

Nanomedicine: Nanotechnology, Biology and Medicine. 2015. Vol. 11, num. 1, p. 239-245. DOI : 10.1016/j.nano.2014.09.007.

NeuroMorph: A Toolset for the Morphometric Analysis and Visualization of 3D Models Derived from Electron Microscopy Image Stacks

A. Jorstad; B. Nigro; C. Cali; M. Wawrzyniak; P. Fua et al. 

Neuroinformatics. 2015. Vol. 13, num. 1, p. 83-92. DOI : 10.1007/s12021-014-9242-5.


Efficient scanning for em based target localization

P. Fua; G. Knott; R. Sznitman; A. Lucchi 

WO2014001157; US8588509.


Nanometer-scale molecular imaging of cerebral metabolic substrate incorporation.

Y. Takado; G. Knott; C. Loussert; B. Humbel; M. Masoodi et al. 

Life Sciences Switzerland Annual Meeting, Lausanne, Switzerland, February 4-5, 2014.

Correlative In Vivo 2-Photon Beam Scanning Electron Microscopy: 3D Analysis of Neuronal Ultrastructure

B. Maco; A. Holtmaat; A. Jorstad; P. Fua; G. Knott 

Correlative Light And Electron Microscopy II; San Diego: Elsevier Academic Press Inc, 2014. p. 339-361.

SAS-1 Is a C2 Domain Protein Critical for Centriole Integrity in C. elegans

L. von Tobel; T. Mikeladze-Dvali; M. Delattre; F. R. Balestra; S. Blanchoud et al. 

Plos Genetics. 2014. Vol. 10, num. 11, p. e1004777. DOI : 10.1371/journal.pgen.1004777.

Conditional expression of Parkinson’s disease-related R1441C LRRK2 in midbrain dopaminergic neurons of mice causes nuclear abnormalities without neurodegeneration

E. Tsika; M. Kannan; C. S-Y. Foo; D. Dikeman; L. Glauser et al. 

Neurobiology Of Disease. 2014. Vol. 71, p. 345-358. DOI : 10.1016/j.nbd.2014.08.027.

Semiautomated correlative 3D electron microscopy of in vivo-imaged axons and dendrites

B. Maco; M. Cantoni; A. Holtmaat; A. Kreshuk; F. A. Hamprecht et al. 

Nature Protocols. 2014. Vol. 9, num. 6, p. 1354-1366. DOI : 10.1038/nprot.2014.101.

New tool for studying the biosynthetic role of glucose in brain at the nanometer scale

Y. Takado; G. Knott; B. M. Humbel; S. Escrig; M. Masoodi et al. 

11th International Conference on Brain Energy Metabolism, Helsinge, Denmark, May 11-14, 2014.

Connexin 30 sets synaptic strength by controlling astroglial synapse invasion

U. Pannasch; D. Freche; G. Dallerac; G. Ghezali; C. Escartin et al. 

Nature Neuroscience. 2014. Vol. 17, num. 4, p. 549-U104. DOI : 10.1038/nn.3662.

FOXO3 determines the accumulation of alpha-synuclein and controls the fate of dopaminergic neurons in the substantia nigra

E. Pino; R. Amamoto; L. Zheng; M. Cacquevel; J-C. Sarria et al. 

Human Molecular Genetics. 2014. Vol. 23, num. 6, p. 1435-1452. DOI : 10.1093/hmg/ddt530.

The Relationship between PSD-95 Clustering and Spine Stability In Vivo

M. Cane; B. Maco; G. Knott; A. Holtmaat 

Journal Of Neuroscience. 2014. Vol. 34, num. 6, p. 2075-2086. DOI : 10.1523/Jneurosci.3353-13.2014.

Pansynaptic Enlargement at Adult Cortical Connections Strengthened by Experience

C. E. J. Cheetham; S. J. Barnes; G. Albieri; G. W. Knott; G. T. Finnerty 

Cerebral Cortex. 2014. Vol. 24, num. 2, p. 521-531. DOI : 10.1093/cercor/bhs334.


Universal sample holder

G. Knott 

US2015022807; WO2013136260.


Is EM dead?

G. Knott; C. Genoud 

Journal Of Cell Science. 2013. Vol. 126, num. 20, p. 4545-4552. DOI : 10.1242/jcs.124123.

BMP signaling specifies the development of a large and fast CNS synapse

L. Xiao; N. Michalski; E. Kronander; E. Gjoni; C. Genoud et al. 

Nature Neuroscience. 2013. Vol. 16, num. 7, p. 856-U109. DOI : 10.1038/nn.3414.

Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neurons

B. Maco; A. Holtmaat; M. Cantoni; A. Kreshuk; C. N. Straehle et al. 

PloS One. 2013. Vol. 8, num. 2, p. e57405. DOI : 10.1371/journal.pone.0057405.

Increased axonal bouton dynamics in the aging mouse cortex

F. W. Grillo; S. Song; T-G. Ruivo; M. Leonor; L. Huang et al. 

Proceedings of the National Academy of Sciences of the United States of America. 2013. Vol. 110, num. 16, p. E1514-23. DOI : 10.1073/pnas.1218731110.

Imaging green fluorescent protein-labeled neurons using light and electron microscopy

G. W. Knott 

Cold Spring Harbor protocols. 2013. Vol. 2013, num. 6, p. 542-50. DOI : 10.1101/pdb.prot075127.

In vivo single branch axotomy induces GAP-43-dependent sprouting and synaptic remodeling in cerebellar cortex

A. Mascaro; A. Letizia; P. Cesare; L. Sacconi; G. Grasselli et al. 

Proceedings of the National Academy of Sciences of the United States of America. 2013. Vol. 110, num. 26, p. 10824-9. DOI : 10.1073/pnas.1219256110.

In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits

A. J. Canty; L. Huang; J. S. Jackson; G. E. Little; G. Knott et al. 

Nature Communications. 2013. Vol. 4, p. 2038. DOI : 10.1038/ncomms3038.

Flash Scanning Electron Microscopy

R. Sznitman; A. Lucchi; M. Cantoni; G. Knott; P. Fua 

2013. 16th Conference on Medical Image Computing and Computer Assisted Intervention (MICCAI), Nagoya, Japan, September 22-26, 2013.

Mitonuclear protein imbalance as a conserved longevity mechanism

R. Houtkooper; L. Mouchiroud; D. Ryu; N. Moullan; E. Katsyuba et al. 

Nature. 2013. Vol. 497, num. 7450, p. 451-457. DOI : 10.1038/nature12188.

Altered Synaptic Dynamics during Normal Brain Aging

R. Mostany; J. E. Anstey; K. L. Crump; B. Maco; G. Knott et al. 

Journal Of Neuroscience. 2013. Vol. 33, num. 9, p. 4094-4104. DOI : 10.1523/Jneurosci.4825-12.2013.

Learning Context Cues for Synapse Segmentation

C. J. Becker; K. Ali; G. Knott; P. Fua 

IEEE Transactions on Medical Imaging. 2013. Vol. 32, num. 10, p. 1864–1877. DOI : 10.1109/Tmi.2013.2267747.


3D ultrastructural study of tissues by serial block-face scanning electron microscopy

C. Genoud; G. Knott 

2012. 39th Annual Meeting of the Society-for-Cutaneous-Ultrastructure-Research (CUR). p. E1-E1.

Efficient Scanning for EM Based Target Localization

R. Sznitman; A. Lucchi; N. Pjescic-Emedi; G. Knott; P. Fua 

2012. 15th International Conference on Medical Image Computing and Computer Assisted Intervention, Nice, France, October, 2012. p. 337-344. DOI : 10.1007/978-3-642-33454-2_42.

Learning Context Cues for Synapse Segmentation in EM Volumes

C. J. Becker; K. Ali; G. Knott; P. Fua 

2012. International Conference on Medical Image Computing and Computer Assisted Intervention (MICCAI). p. 585-592. DOI : 10.1007/978-3-642-33415-3_72.

Analysis of centriole elimination during C. elegans oogenesis

T. Mikeladze-Dvali; L. von Tobel; P. Strnad; G. Knott; H. Leonhardt et al. 

Development. 2012. Vol. 139, num. 9, p. 1670-1679. DOI : 10.1242/dev.075440.

Nigrostriatal overabundance of alpha-synuclein leads to decreased vesicle density and deficits in dopamine release that correlate with reduced motor activity

M. N. Gaugler; O. Genc; W. Bobela; S. Mohanna; M. T. Ardah et al. 

Acta Neuropathologica. 2012. Vol. 123, num. 5, p. 653-669. DOI : 10.1007/s00401-012-0963-y.

GABA Signaling Promotes Synapse Elimination and Axon Pruning in Developing Cortical Inhibitory Interneurons

X. Wu; Y. Fu; G. Knott; J. Lu; G. Di Cristo et al. 

Journal Of Neuroscience. 2012. Vol. 32, p. 331-343. DOI : 10.1523/JNEUROSCI.3189-11.2012.

Supervoxel-Based Segmentation of Mitochondria in EM Image Stacks with Learned Shape Features

A. Lucchi; K. Smith; R. Achanta; G. Knott; P. Fua 

IEEE Transactions on Medical Imaging. 2012. Vol. 31, num. 2, p. 474-486. DOI : 10.1109/TMI.2011.2171705.


Focused Ion Beam Nano-Tomography Using Different Detectors

M. Cantoni; P. Burdet; G. Knott; C. Hébert 

Microscopy and Microanalysis. 2011. Vol. 17, num. S2, p. 882-883. DOI : 10.1017/S1431927611005289.

Automated Detection and Segmentation of Synaptic Contacts in Nearly Isotropic Serial Electron Microscopy Images

A. Kreshuk; C. N. Straehle; C. Sommer; U. Koethe; M. Cantoni et al. 

Plos One. 2011. Vol. 6, num. 10, p. e24899. DOI : 10.1371/journal.pone.0024899.


FIB-SEM Nanotomography in Materials and Life Science at EPFL

M. Cantoni; G. Knott; C. Hébert 

Microscopy and Microanalysis. 2010. Vol. 16, num. S2, p. 226-227. DOI : 10.1017/S1431927610059623.

Alpha-Synuclein Animal Models

M. Gaugler; P. G. Coune; S. Mohana; O. Genc; O. El-Agnaf et al. 

2010. 2nd World Parkinson Congress, Glasgow, UK, September 28 – October 1, 2010. p. S606-S606.

Supervoxel-Based Segmentation of EM Image Stacks with Learned Shape Features

A. Lucchi; K. Smith; R. Achanta; G. Knott; P. Fua 



High-Throughput 3D Cellular Imaging

G. Knott; D. Wall; B. Lich 

Microscopy and Microanalysis. 2009. Vol. 15, num. S2, p. 934-935. DOI : 10.1017/S1431927609097037.

A Single Cell Approach for Safe Gene Therapy

S. Lathion; A. Rochat; G. Knott; A. Recchia; M-A. Zanta-Boussif et al. 

2009. 8th Annual Meeting of the French-Society-of-Cell-and-Gene-Therapy, Paris, FRANCE, Jun 21-23, 2009. p. 689-690.

A protocol for preparing GFP-labeled neurons previously imaged in vivo and in slice preparations for light and electron microscopic analysis

G. W. Knott; A. Holtmaat; J. T. Trachtenberg; K. Svoboda; E. Welker 

Nature Protocols. 2009. Vol. 4, p. 1145-1156. DOI : 10.1038/nprot.2009.114.