The true shape of dendritic spines – December 2020
Using cryo fixation, the BioEM facility shows how dendritic spines are altered by chemical fixation. The dendritic spine is the site where the majority of excitatory connections are found in the adult brain. There size and shape are important determinants of their function. A detailed morphological analysis now published in eLife shows few changes, however, cryo-fixed spines have significantly thinner necks.
TEM in Parkinson’s disease
The group of Hilal Lashuel publishes an extensive study in the journal PNAS in which the progression of Lewy body formation is tracked in cells. This includes transmission electron microscopy with immunocytochemistry. See the EPFL news here. The research article can be viewed here.
Block face scanning EM in Alzheimer’s Disease
A study lead by Gilles Bonvento (Paris) explores astrocytic metabolism and mechanistic link that connects defective glycolysis in astrocytes to impaired l-serine production and reduced neuronal function. Supplementation with L-serine in a mouse model of Alzheimer’s restores cognitive deficits. This study used the block-face scanning electron microscopy services offered by the BioEM Facility. The study is published in the journal Cell Metabolism, and can be viewed here.
Nuclear and cytoplasmic huntingtin inclusions exhibit distinct biochemical composition, interactome and ultrastructural propertiesNature Communications. 2021-11-12. Vol. 12, num. 1, p. 6579. DOI : 10.1038/s41467-021-26684-z.
Gas cluster ion beam SEM for imaging of large tissue samples with 10 nm isotropic resolutionNature Methods. 2020-11-18. Vol. 17, num. 1, p. 68-71. DOI : 10.1038/s41592-019-0641-2.
Impairment of Glycolysis-Derived L-Serine Production in Astrocytes Contributes to Cognitive Deficits in Alzheimer’s DiseaseCell Metabolism. 2020-03-03. Vol. 31, num. 3, p. 503-517.e8. DOI : 10.1016/j.cmet.2020.02.004.
Voxel2Mesh: 3D Mesh Model Generation from Volumetric Data2020. International Conference On Medical Image Computing & Computer Assisted Intervention (MICCAI), Lima, Peru, 4-8 OCTOBER 2020.
Synaptic proximity enables NMDAR signalling to promote brain metastasisNature. 2019-09-26. Vol. 573, num. 7775, p. 526-531. DOI : 10.1038/s41586-019-1576-6.
The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During AgingMolecular Cell. 2019-02-21. Vol. 73, num. 4, p. 775-787.e10. DOI : 10.1016/j.molcel.2018.11.034.
Diversity of Cortico-descending Projections: Histological and Diffusion MRI Characterization in the MonkeyCerebral Cortex. 2019-02-01. Vol. 29, num. 2, p. 788-801. DOI : 10.1093/cercor/bhx363.
In vivo modeling of human neuron dynamics and Down syndromeScience. 2018-11-16. Vol. 362, num. 6416, p. eaau1810. DOI : 10.1126/science.aau1810.
Ultrastructural basis of strong unitary inhibition in a binaural neuronJournal Of Physiology-London. 2018-10-15. Vol. 596, num. 20, p. 4969-4982. DOI : 10.1113/JP276015.
Locally coordinated synaptic plasticity of visual cortex neurons in vivoScience. 2018-06-22. Vol. 360, num. 6395, p. 1349-1354. DOI : 10.1126/science.aao0862.
The effects of aging on neuropil structure in mouse somatosensory cortex-A 3D electron microscopy analysis of layer 1PLOS ONE. 2018. Vol. 13, num. 7, p. e0198131. DOI : 10.1371/journal.pone.0198131.
Neuron Segmentation With High-Level Biological PriorsIEEE TRANSACTIONS ON MEDICAL IMAGING. 2018. Vol. 37, num. 4, p. 829-839. DOI : 10.1109/TMI.2017.2712360.
Computer assisted detection of axonal bouton structural plasticity in in vivo time-lapse imagesElife. 2017. Vol. 6, p. e29315. DOI : 10.7554/eLife.29315.
Homeostatic control of dopamine by astrocytes in the postnatal maturation of the prefrontal cortex2017. ISN-ESN Meeting, Paris, FRANCE, AUG 20-24, 2017. p. 18-18.
Identification of aminopyrimidine-sulfonamides as potent modulators of Wag31-mediated cell elongation in mycobacteriaMolecular Microbiology. 2017. Vol. 103, num. 1, p. 13-25. DOI : 10.1111/mmi.13535.
Multicut brings automated neurite segmenation closer to human performanceNature Methods. 2017. Vol. 14, num. 2, p. 101-102. DOI : 10.1038/nmeth.4151.
Multi-Modal Optical Imaging of the Cerebellum in Animals2016. 7th International Symposium of the Society-for-Research-on-the-Cerebellum (SRC), Brussels, BELGIUM, MAY 08-10, 2015. p. 18-20. DOI : 10.1007/s12311-015-0730-4.
NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitroChemical Communications. 2015. Vol. 51, num. 92, p. 16577-16577. DOI : 10.1039/c5cc90487k.
NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitroChemical Communications. 2015. Vol. 51, num. 92, p. 16486-16489. DOI : 10.1039/c5cc06983a.
Imaging the time-integrated cerebral metabolic activity with subcellular resolution through nanometer-scale detection of biosynthetic products deriving from (13)C-glucoseJournal of chemical neuroanatomy. 2015. Vol. 69, p. 7-12. DOI : 10.1016/j.jchemneu.2015.09.003.
Delayed and Temporally Imprecise Neurotransmission in Reorganizing Cortical MicrocircuitsJournal Of Neuroscience. 2015. Vol. 35, num. 24, p. 9024-9037. DOI : 10.1523/Jneurosci.4583-14.2015.
Multiphoton microscopy in brain imaging2015. Conference on Multiphoton Microscopy in the Biomedical Sciences XV, San Francisco, CA, FEB 08-10, 2015. DOI : 10.1117/12.2084183.
Cold shock protects the brainNature. 2015. Vol. 518, num. 7538, p. 177-178. DOI : 10.1038/nature14195.
Ultrastructurally smooth thick partitioning and volume stitching for large-scale connectomicsNature Methods. 2015. Vol. 12, num. 4, p. 319-U55. DOI : 10.1038/Nmeth.3292.
Imaging liver and brain glycogen metabolism at the nanometer scaleNanomedicine: Nanotechnology, Biology and Medicine. 2015. Vol. 11, num. 1, p. 239-245. DOI : 10.1016/j.nano.2014.09.007.
Efficient scanning for em based target localization
Nanometer-scale molecular imaging of cerebral metabolic substrate incorporation.Life Sciences Switzerland Annual Meeting, Lausanne, Switzerland, February 4-5, 2014.
Conditional expression of Parkinson’s disease-related R1441C LRRK2 in midbrain dopaminergic neurons of mice causes nuclear abnormalities without neurodegenerationNeurobiology Of Disease. 2014. Vol. 71, p. 345-358. DOI : 10.1016/j.nbd.2014.08.027.
Semiautomated correlative 3D electron microscopy of in vivo-imaged axons and dendritesNature Protocols. 2014. Vol. 9, num. 6, p. 1354-1366. DOI : 10.1038/nprot.2014.101.
New tool for studying the biosynthetic role of glucose in brain at the nanometer scale11th International Conference on Brain Energy Metabolism, Helsinge, Denmark, May 11-14, 2014.
Connexin 30 sets synaptic strength by controlling astroglial synapse invasionNature Neuroscience. 2014. Vol. 17, num. 4, p. 549-U104. DOI : 10.1038/nn.3662.
The Relationship between PSD-95 Clustering and Spine Stability In VivoJournal Of Neuroscience. 2014. Vol. 34, num. 6, p. 2075-2086. DOI : 10.1523/Jneurosci.3353-13.2014.
Universal sample holder
Is EM dead?Journal Of Cell Science. 2013. Vol. 126, num. 20, p. 4545-4552. DOI : 10.1242/jcs.124123.
BMP signaling specifies the development of a large and fast CNS synapseNature Neuroscience. 2013. Vol. 16, num. 7, p. 856-U109. DOI : 10.1038/nn.3414.
Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical neuronsPloS One. 2013. Vol. 8, num. 2, p. e57405. DOI : 10.1371/journal.pone.0057405.
Increased axonal bouton dynamics in the aging mouse cortexProceedings of the National Academy of Sciences of the United States of America. 2013. Vol. 110, num. 16, p. E1514-23. DOI : 10.1073/pnas.1218731110.
Imaging green fluorescent protein-labeled neurons using light and electron microscopyCold Spring Harbor protocols. 2013. Vol. 2013, num. 6, p. 542-50. DOI : 10.1101/pdb.prot075127.
In vivo single branch axotomy induces GAP-43-dependent sprouting and synaptic remodeling in cerebellar cortexProceedings of the National Academy of Sciences of the United States of America. 2013. Vol. 110, num. 26, p. 10824-9. DOI : 10.1073/pnas.1219256110.
In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuitsNature Communications. 2013. Vol. 4, p. 2038. DOI : 10.1038/ncomms3038.
Flash Scanning Electron Microscopy2013. 16th Conference on Medical Image Computing and Computer Assisted Intervention (MICCAI), Nagoya, Japan, September 22-26, 2013.
Mitonuclear protein imbalance as a conserved longevity mechanismNature. 2013. Vol. 497, num. 7450, p. 451-457. DOI : 10.1038/nature12188.
Altered Synaptic Dynamics during Normal Brain AgingJournal Of Neuroscience. 2013. Vol. 33, num. 9, p. 4094-4104. DOI : 10.1523/Jneurosci.4825-12.2013.
3D ultrastructural study of tissues by serial block-face scanning electron microscopy2012. 39th Annual Meeting of the Society-for-Cutaneous-Ultrastructure-Research (CUR). p. E1-E1.
Analysis of centriole elimination during C. elegans oogenesisDevelopment. 2012. Vol. 139, num. 9, p. 1670-1679. DOI : 10.1242/dev.075440.
GABA Signaling Promotes Synapse Elimination and Axon Pruning in Developing Cortical Inhibitory InterneuronsJournal Of Neuroscience. 2012. Vol. 32, p. 331-343. DOI : 10.1523/JNEUROSCI.3189-11.2012.
Automated Detection and Segmentation of Synaptic Contacts in Nearly Isotropic Serial Electron Microscopy ImagesPlos One. 2011. Vol. 6, num. 10, p. e24899. DOI : 10.1371/journal.pone.0024899.
Alpha-Synuclein Animal Models2010. 2nd World Parkinson Congress, Glasgow, UK, September 28 – October 1, 2010. p. S606-S606.
Supervoxel-Based Segmentation of EM Image Stacks with Learned Shape Features