DNA Origami nanostructures for controlled therapeutic drug delivery
Nucleic acids presenting polymer nanomaterials as vaccine adjuvants.
Alice Comberlato, Kaltrina Paloja and Maartje M.C. Bastings, Journal of Materials Chemistry B, 2019. 10.1039/C9TB01222B.
Large‐Range HS‐AFM Imaging of DNA Self‐Assembly through In Situ Data‐Driven Control
Adrian P. Nievergelt, Christoph Kammer, Charlène Brillard, Eva Kurisinkal, Maartje M. C. Bastings, Alireza Karimi and Georg E. Fantner, Small Methods, 2019. 10.1002/smtd.201900031.
Quantifying Guest–Host Dynamics in Supramolecular Assemblies to Analyze Their Robustness.
Maartje M.C. Bastings, Thomas M. Hermans, A. J. H. Spiering, Erwin W. L. Kemps, Lorenzo Albertazzi, Eva E. Kurisinkal, Patricia Y. W. Dankers, Macromol. Biosci. 2018, 1800296. 10.1002/mabi.201800296.
Engineering a stable future for DNA-origami as biomaterial.
Hale Bila, Eva E. Kurisinkal, Maartje M.C. Bastings, Biomater. Sci., 2018. 10.1039/C8BM01249K.
Emergent Investigators Special Edition
DNA as biomaterial has evoked great interest as potential platform for therapeutics, diagnostics and as hydrogel scaffolds due to the relative ease of programming their robust and uniform shape, site-specific functionality and controlled responsive behavior. However, for a stable self-assembled product, a relatively high cation concentration is required to prevent denaturation. Physiological and cell-culture conditions do not match these concentrations and present additional nucleases that form a serious threat to the integrity of DNA-based materials. For the translation of this promising technology toward bioengineering challenges, the stability needs to be guaranteed. Over the past years, various methods have been developed addressing the stability-related weaknesses of DNA-origami. This mini-review explains the common stability issues and compares the stabilization strategies recently developed. We present a detailed overview of each method in order to ease the selection-process on which method to use for future users of DNA-origami as biomaterial.
Modulation of cellular uptake of DNA origami through control over mass and shape.
M.M.C. Bastings, N. Ponnuswamy, F.M. Anastassacos, F. Graf, G. Cuneo, C. Lin, D.E. Ingber, J.H. Ryu, W.M. Shih; 2018, Nano Letters, 18, 6, 3557-3564
Oligolysine-based coating protects DNA nanostructures from low-salt denaturation and nuclease degradation.
N. Ponnuswamy, M.M.C. Bastings, B. Nathwani, M. Vinther, A. Li, D.J. Mooney, W.M. Shih; 2017, Nature Communications, 8, 15654
Programmable control in extracellular matrix mimicking polymer hydrogels.
Hof, M.M.C. Bastings, 2017, CHIMIA International Journal for Chemistry, 71, 6, 342-348
Full list of publications here