Competence-mediated DNA uptake diversifies Vibrio cholerae sedentary chromosomal integrons

L. Righi; S. Stutzmann; L. Bader; A. Lemopoulos; M. Blokesch 

Deciphering interbacterial killing mediated by a conjugative plasmid in Vibrio cholerae

Large-scale recovery of integron cassettes for gene discovery screens

F. Trigo da Roza; A. Carvalho; A. Prieto; P. Blanco; E. Vergara et al. 

Structure and activation mechanism of a Lamassu phage and plasmid defense system

Y. Li; D. W. Adams; H. W. Liu; S. J. Shaw; E. Uchikawa et al. 

West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems

D. W. Adams; M. Jaskólska; A. Lemopoulos; S. Stutzmann; L. Righi et al. 

Exploring Mobile Genetic Elements in Vibrio cholerae

N. C. Drebes Dörr; A. Lemopoulos; M. Blokesch 

Capsular polysaccharide restrains type VI secretion in Acinetobacter baumannii

N. Flaugnatti; L. Bader; M. Croisier-Coeytaux; M. Blokesch 

Defence systems encoded by core genomic islands of seventh pandemic Vibrio cholerae

M. Blokesch 

Lineage-specific defence systems of pandemic Vibrio cholerae

M. Blokesch; K. D. Seed 

Synthetically designed anti-defense proteins overcome barriers to bacterial transformation and phage infection

J. Garb; D. W. Adams; E. H. Yardeni; M. Blokesch; R. Sorek 

Structure and Activation Mechanism of a Lamassu Phage Defence System

Y. Li; D. W. Adams; Liu; S. J. Shaw; E. Uchikawa et al. 

Vibrio cholerae pathogenicity island 2 encodes two distinct types of restriction systems

G. Vizzarro; A. Lemopoulos; D. W. Adams; M. Blokesch 

Genome sequences of Vibrio cholerae strains isolated in the DRC between 2009 and 2012

A. Lemopoulos; B. Miwanda; N. C. Drebes Dorr; S. Stutzmann; D. Bompangue et al. 

Interactions between pili affect the outcome of bacterial competition driven by the type VI secretion system

S. B. Otto; R. M. Servajean; A. Lemopoulos; A-F. Bitbol; M. Blokesch 

Molecular mechanism of plasmid elimination by the DdmDE defense system

L. Loeff; D. W. Adams; C. Chanez; S. Stutzmann; L. Righi et al. 

Q & A – Melanie Blokesch

M. Blokesch 

Dangerous acquaintances: the interplay between type IV pili and the type VI secretion system during Vibrio cholerae’s environmental lifestyle

Diverse phage defence systems define West African South American pandemic Vibrio cholerae

D. W. Adams; M. Jaskólska; A. Lemopoulos; S. Stutzmann; L. Righi et al. 

Vibrio cholerae pathogenicity island 2 encodes two distinct types of restriction systems

G. Vizzarro; A. Lemopoulos; D. W. Adams; M. Blokesch 

Capsular Polysaccharide Restrains Type VI Secretion in Acinetobacter baumannii

N. O. Flaugnatti; L. Bader; M-C. Croisier-Coeytaux; M. Blokesch 

Sporadic type VI secretion in seventh pandemic Vibrio cholerae

A. Proutière; N. C. Drebes Dorr; L. Bader; S. Stutzmann; L. C. Metzger et al. 

DNA modifications impact natural transformation of Acinetobacter baumannii

N. Vesel; C. Iseli; N. Guex; A. Lemopoulos; M. Blokesch 

Interactions between pili affect the outcome of bacterial competition driven by the type VI secretion system

S. B. Otto; R. M. Servajean; A. Lemopoulos; A-F. Bitbol; M. Blokesch 

Recombinant bacteria resistant to horizontal gene transfer and phage infection

M. Blokesch; M. J. Jaskolska; D. W. Adams 

Single nucleotide polymorphism determines constitutive versus inducible type VI secretion in Vibrio cholerae

N. C. Drebes Dörr; A. Proutière; M. Jaskólska; S. Stutzmann; L. Bader et al. 

Two defence systems eliminate plasmids from seventh pandemic Vibrio cholerae

M. Jaskólska; D. W. Adams; M. Blokesch 

Solving the mystery of the missing plasmids in seventh pandemic Vibrio cholerae strains

M. Blokesch 

The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae

L. F. Lemos Rocha; K. Peters; J. Biboy; J. S. Depelteau; A. Briegel et al. 

How Vibrio cholerae adapts to the environment: from cell shape transitions to antagonistic behavior

Natural competence of the pathogen Acinetobacter baumannii: an elusive phenomenon

Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms

N. Flaugnatti; S. Isaac; L. F. Lemos Rocha; S. Stutzmann; O. Rendueles et al. 

Pilus production in Acinetobacter baumannii is growth phase dependent and essential for natural transformation

N. Vesel; M. Blokesch 

Growing away from monocultures – interdependent growth conditions for studying antibacterial and antiphage systems

M. Blokesch 

The molecular treasure box of environmental Vibrio cholerae strains

Interbacterial competition and anti‐predatory behavior of environmental Vibrio cholerae strains

N. C. Drebes Dörr; M. Blokesch 

Comparison of chitin‐induced natural transformation in pandemic Vibrio cholerae O1 El Tor strains

S. Stutzmann; M. Blokesch 

A Vibriophage Takes Antirepression to the Next Level

L. F. Lemos Rocha; M. Blokesch 

Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells

D. Piel; M. Bruto; A. James; Y. Labreuche; C. Lambert et al. 

The type IV pilus protein PilU functions as a PilT-dependent retraction ATPase

D. W. Adams; J. M. Pereira; C. Stoudmann; S. Stutzmann; M. Blokesch 

Neighbor predation linked to natural competence fosters the transfer of large genomic regions in Vibrio cholerae

N. Matthey; S. Stutzmann; C. Stoudmann; N. Guex; C. Iseli et al. 

DNA-uptake pili of Vibrio cholerae are required for chitin colonization and capable of kin recognition via sequence-specific self-interaction

D. W. Adams; S. Stutzmann; C. Stoudmann; M. Blokesch 

Ecological implications of gene regulation by TfoX and TfoY among diverse Vibrio species

L. C. Metzger; N. Matthey; C. Stoudmann; E. J. Collas; M. Blokesch 

Cellular Microbiology Interview – Dr. Melanie Blokesch

M. Blokesch 

Mechanisms of DNA Uptake by Naturally Competent Bacteria

D. Dubnau; M. Blokesch 

Two nanomachines drive evolution in diverse Vibrio species

Long-Read-Based Genome Sequences of Pandemic and Environmental Vibrio cholerae Strains

N. Matthey; N. C. Drebes Dörr; M. Blokesch 

Molecular insights into Vibrio cholerae’s intra-amoebal host-pathogen interactions

C. Van der Henst; A. S. Vanhove; N. C. Drebes Dörr; S. Stutzmann; C. Stoudmann et al. 

Bacterial type VI secretion system facilitates niche domination

N. C. Drebes Dörr; M. Blokesch 

QstR-dependent regulation of natural competence and type VI secretion in Vibrio cholerae

M. Jaskolska; S. Stutzmann; C. Stoudmann; M. Blokesch 

Eco-Evolutionary Dynamics Linked to Horizontal Gene Transfer in Vibrios

F. Le Roux; M. Blokesch 

Interbacterial predation as a strategy for DNA acquisition in naturally competent bacteria

J-W. Veening; M. Blokesch 

Modeling Key Drivers of Cholera Transmission Dynamics Provides New Perspectives for Parasitology

A. Rinaldo; E. Bertuzzo; M. Blokesch; L. Mari; M. Gatto 

In and out—contribution of natural transformation to the shuffling of large genomic regions

M. Blokesch 

Circulation of a Quorum-Sensing-Impaired Variant of Vibrio cholerae Strain C6706 Masks Important Phenotypes

S. Stutzmann; M. Blokesch 

Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY

L. C. Metzger; S. Stutzmann; T. Scrignari; C. Van der Henst; N. Matthey et al. 

Regulation of competence-mediated horizontal gene transfer in the natural habitat of Vibrio cholerae

L. C. Metzger; M. Blokesch 

The DNA-Uptake Process of Naturally Competent Vibrio cholerae

N. Matthey; M. Blokesch 

Natural competence for transformation

M. Blokesch 

An intracellular replication niche for Vibrio cholerae in the amoeba Acanthamoeba castellanii

C. Van Der Henst; T. Scrignari; C. Maclachlan; M. Blokesch 

The emergence of Vibrio pathogens in Europe: ecology, evolution, and pathogenesis (Paris, 11–12th March 2015)

F. Le Roux; K. M. Wegner; C. Baker-Austin; L. Vezzulli; C. R. Osorio et al. 

The type VI secretion system of Vibrio cholerae fosters horizontal gene transfer

S. Borgeaud; L. C. Metzger; T. Scrignari; M. Blokesch 

Leben und sterben lassen – horizontaler Gentransfer in Vibrio cholerae

M. Blokesch 

Competence-induced type VI secretion might foster intestinal colonization by Vibrio cholerae

M. Blokesch 

Protocols for Visualizing Horizontal Gene Transfer in Gram-Negative Bacteria Through Natural Competence

M. Blokesch 

Composition of the DNA-uptake complex of Vibrio cholerae

L. C. Metzger; M. Blokesch 

Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae

M. Lo Scrudato; S. Borgeaud; M. Blokesch 

ComEA Is Essential for the Transfer of External DNA into the Periplasm in Naturally Transformable Vibrio cholerae Cells

P. Seitz; H. Pezeshgi Modarres; S. Borgeaud; R. D. Bulushev; L. J. Steinbock et al. 

Probing the size of proteins with glass nanopores

L. J. Steinbock; S. Krishnan; R. D. Bulushev; S. Borgeaud; M. Blokesch et al. 

DNA Transport across the Outer and Inner Membranes of Naturally Transformable Vibrio cholerae Is Spatially but Not Temporally Coupled

P. Seitz; M. Blokesch 

Glucose- but Not Rice-Based Oral Rehydration Therapy Enhances the Production of Virulence Determinants in the Human Pathogen Vibrio cholerae

J. Kühn; F. Finger; E. Bertuzzo; S. Borgeaud; M. Gatto et al. 

Mechanistic aspects of DNA uptake in naturally competent Vibrio cholerae

The regulatory circuit of natural competence for transformation in the human pathogen Vibrio cholerae

The Janthinobacterium sp. HH01 Genome Encodes a Homologue of the V. cholerae CqsA and L. pneumophila LqsA Autoinducer Synthases

C. Hornung; A. Poehlein; F. S. Haack; M. Schmidt; K. Dierking et al. 

Overexpression of the tcp Gene Cluster Using the T7 RNA Polymerase/Promoter System and Natural Transformation-Mediated Genetic Engineering of Vibrio cholerae

S. Borgeaud; M. Blokesch 

DNA-uptake machinery of naturally competent Vibrio cholerae

P. Seitz; M. Blokesch 

Evidence for Two Different Regulatory Mechanisms Linking Replication and Segregation of Vibrio cholerae Chromosome II

T. Venkova-Canova; J. H. Baek; P. C. Fitzgerald; M. Blokesch; D. K. Chattoraj 

A transcriptional regulator linking quorum sensing and chitin induction to render Vibrio cholerae naturally transformable

M. Lo Scrudato; M. Blokesch 

Cues and regulatory pathways involved in natural competence and transformation in pathogenic and environmental Gram-negative bacteria

P. Seitz; M. Blokesch 

A quorum sensing-mediated switch contributes to natural transformation of Vibrio cholerae

M. Blokesch 

The Regulatory Network of Natural Competence and Transformation of Vibrio cholerae

M. Lo Scrudato; M. Blokesch 

Chitin colonization, chitin degradation and chitin-induced natural competence of Vibrio cholerae are subject to catabolite repression

M. Blokesch 

TransFLP – A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

M. Blokesch 

Reassessment of the 2010-2011 Haiti cholera outbreak and rainfall-driven multiseason projections

A. Rinaldo; E. Bertuzzo; L. Mari; L. Righetto; M. Blokesch et al. 

Quorum Sensing Contributes to Natural Transformation of Vibrio cholerae in a Species-Specific Manner

G. Suckow; P. Seitz; M. Blokesch 

A transmission model of the 2010 cholera epidemic in haiti

A. Rinaldo; M. Blokesch; E. Bertuzzo; L. Mari; L. Righetto et al. 

Prediction of the spatial evolution and effects of control measures for the unfolding Haiti cholera outbreak

E. Bertuzzo; L. Mari; L. Righetto; M. Gatto; R. Casagrandi et al. 

Genetic manipulation of Vibrio cholerae by combining natural transformation with FLP recombination

O. De Souza Silva; M. Blokesch 

Natural transformation of Vibrio cholerae as a tool – Optimizing the procedure

R. L. Marvig; M. Blokesch 

The extracellular nuclease Dns and its role in natural transformation of Vibrio cholerae

M. Blokesch; G. K. Schoolnik 

Serogroup conversion of Vibrio cholerae in aquatic reservoirs

M. Blokesch; G. K. Schoolnik 

Properties of the [NiFe]-hydrogenase maturation protein HypD

M. Blokesch; A. Böck 

Maturation of hydrogenases

A. Böck; P. W. King; M. Blokesch; M. C. Posewitz 

Chitin induces natural competence in Vibrio cholerae

K. L. Meibom; M. Blokesch; N. A. Dolganov; C-Y. Wu; G. K. Schoolnik 

The biosynthetic routes for carbon monoxide and cyanide in the Ni-Fe active site of hydrogenases are different

W. Roseboom; M. Blokesch; A. Böck; S. P. J. Albracht 

Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE

M. Blokesch; A. Paschos; A. Bauer; S. Reissmann; N. Drapal et al. 

HybF, a zinc-containing protein involved in NiFe hydrogenase maturation

M. Blokesch; M. Rohrmoser; S. Rode; A. Böck 

The complex between hydrogenase-maturation proteins HypC and HypD is an intermediate in the supply of cyanide to the active site iron of [NiFe]-hydrogenases

M. Blokesch; S. P. J. Albracht; B. F. Matzanke; N. M. Drapal; A. Jacobi et al. 

Maturation of [NiFe]-hydrogenases in Escherichia coli: the HypC cycle

M. Blokesch; A. Böck 

Network of hydrogenase maturation in Escherichia coli: role of accessory proteins HypA and HybF

M. Hube; M. Blokesch; A. Böck 

Metal insertion into NiFe-hydrogenases

M. Blokesch; A. Paschos; E. Theodoratou; A. Bauer; M. Hube et al. 

Fidelity of metal insertion into hydrogenases

A. Magalon; M. Blokesch; E. Zehelein; A. Böck 

Interplay between the specific chaperone-like proteins HybG and HypC in maturation of hydrogenases 1, 2, and 3 from Escherichia coli

M. Blokesch; A. Magalon; A. Böck