NEW: Please see our new tool CloneMap here.
We manage and distribute several large collections of ORFs:
- The “ORFeome Collaboration” (OC). Untagged human ORFs. Promoterless, but ready for shuttling into Gateway expression vectors.
- The “CCSB-Broad Lentiviral Expression Library” (CB-Lenti). Gateway-compatible, CMV promoter-driven, V5-tagged human ORFs, ready for expression by lentiviral vector transduction or simple transfection.
- Small collections of human and murine of ORFs, called CAHOS and MOUSAIC respectively. We assemble these from different sources to fill in the gaps of our main ORFs collections. When possible we favor Gateway-compatible constructs.
Key points regarding these collections:
- Overall our collections currently represent ~13’400 unique human protein-coding genes. According to recent estimates, the human genome contains 19’000-21’000 protein-coding genes.
- Our collections often include several ORFs for a given gene, typically different isoforms or natural variants.
- If you want to share ORFs with GECF, please contact us.
- Some ORFs in commercially-available collections either do not represent canonical isoforms, or contain potentially deleterious SNPs, or are truncated/mutated. More information on how we can help with that HERE.
- Most of our clones are inserted in vectors compatible with the Gateway system (Invitrogen), allowing easy shuttling of ORFs across collections or towards new Gateway-compatible vectors.
- For information and tips about handling the clones, please see HERE.
- For details about ordering procedure or billing, please see HERE.