Open Resources

Molecular Reagents

MODULAR DROSOPHILA TRANSGENESIS VECTORS

The ability to reproducibly target expression of transgenes to defined subsets of cells is a critical experimental tool for understanding many biological processes. We have designed and developed a modular Drosophila vector series, pBID, to optimize and accelerate the production and analysis of Drosophila transgenes. Our vectors allow 1. phiC31 targeted transgene integration, 2. incorporate insulator sequences and an artificial minimal promoter to ensure specific and uniform transgene expression, 3. allow easy mini-white selection and 4. utilize Gateway recombination cloning technology to enable high-throughput assembly (some vectors are also available in restriction cloning variants). A complete description of the vector design and evaluation is published here. All vectors are available from the nonprofit Addgene repository here or upon request if necessary. You can download maps and sequences of the entire pBID1 series in FASTA format here or Snapgene format here.

Newly Available: pVID – An ‘All-in-One‘ template vector backbone for use with CRISPR/Cas9 based genome editing employing Homology Directed Repair (HDR). Enables the cloning of the template sequences for HDR-based genome editing plus the cognate gRNA sequences in one single vector. Importantly this vector enables the selection against edits with undesired HDR template vector genome incorporation and mutagenesis in Drosophila – read about this very frequent problem here. You can download a map and sequence of pVID in FASTA and Snapgene formats here. pVID is available from the Addgene repository here or upon request if necessary.

OTHER CONSTRUCTS AND REAGENTS

All other constructs or other reagents generated in any of our publications are available freely upon request.

Code

Custom code we have generated for our analysis of microscopy and behavioral data in any of our publications is freely available to download here