Publications

2024

Journal Articles

Modified capillary number to standardize droplet generation in suction-driven microfluidics

J. Panwar; R. Roy 

Microfluidics And Nanofluidics. 2024-04-01. Vol. 28, num. 4, p. 23. DOI : 10.1007/s10404-024-02714-2.

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Patents

Improved microfluidic transcriptome based screening methods

H. Hu; C. Merten; X. Ma; L. Kolmar 

WO2024047028; EP4332234.

2024.

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Fluorometer system, fluorescene analysis method, and use thereof

C. Merten; J. Panwar 

EP4306929.

2024.

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2023

Journal Articles

Screening for drivers of SARS-CoV-2 uptake

A. L. Autour; C. A. Merten 

Nature Biomedical Engineering. 2023-12-29. DOI : 10.1038/s41551-023-01170-7.

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iSort enables automated complex microfluidic droplet sorting in an effort to democratize technology

J. Panwar; R. Utharala; L. Fennelly; D. Frenzel; C. A. Merten 

Cell Reports Methods. 2023-05-22. Vol. 3, num. 5, p. 100478. DOI : 10.1016/j.crmeth.2023.100478.

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Fluorescence crosstalk reduction by modulated excitation-synchronous acquisition for multispectral analysis in high-throughput droplet microfluidics

J. Panwar; C. A. A. Merten 

Lab On A Chip. 2023-05-18. Vol. 23, num. 11. DOI : 10.1039/d2lc01016j.

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Design and construction of a microfluidics workstation for high-throughput multi-wavelength fluorescence and transmittance activated droplet analysis and sorting

J. Panwar; A. Autour; C. A. Merten 

Nature Protocols. 2023-01-27. DOI : 10.1038/s41596-022-00796-2.

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Patents

Automated droplet manipulation in microfluidic systems

J. Panwar; R. Utharala; C. Merten; L. Fennelly 

WO2023094702.

2023.

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2022

Journal Articles

Differential contribution of nuclear size scaling mechanisms between Xenopus species

H. Heijo; C. A. Merten; Y. Hara 

Development Growth & Differentiation. 2022-11-05. DOI : 10.1111/dgd.12819.

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A microfluidic Braille valve platform for on-demand production, combinatorial screening and sorting of chemically distinct droplets

R. Utharala; A. Grab; V. Vafaizadeh; N. Peschke; M. Ballinger et al. 

Nature Protocols. 2022-10-19. DOI : 10.1038/s41596-022-00740-4.

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Combi-seq for multiplexed transcriptome-based profiling of drug combinations using deterministic barcoding in single-cell droplets

L. Mathur; B. Szalai; N. H. Du; R. Utharala; M. Ballinger et al. 

Nature Communications. 2022-08-01. Vol. 13, num. 1, p. 4450. DOI : 10.1038/s41467-022-32197-0.

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Enrichment of gut microbiome strains for cultivation-free genome sequencing using droplet microfluidics

A. Pryszlak; T. Wenzel; K. W. Seitz; F. Hildebrand; E. Kartal et al. 

Cell Reports Methods. 2022-01-24.  p. 100137. DOI : 10.1016/j.crmeth.2021.100137.

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Reviews

Technological and computational advances driving high-throughput oncology

L. Kolmar; A. Autour; X. Ma; B. Vergier; F. Eduati et al. 

Trends In Cell Biology. 2022-10-13. Vol. 32, num. 11, p. 947-961. DOI : 10.1016/j.tcb.2022.04.008.

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Patents

High complexity microcompartment-based interaction screening

C. Merten; L. Kolmar; A. L. Autour; Y. F. S. Seah; A. Mei et al. 

EP4330678; WO2022229205.

2022.

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Quantification of successful encapsulation into microfluidic compartments

C. Merten; A. Grab 

EP4327096; WO2022223782; EP4080208.

2022.

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2020

Journal Articles

Targeted Perturb-seq enables genome-scale genetic screens in single cells

D. Schraivogel; A. R. Gschwind; J. H. Milbank; D. R. Leonce; P. Jakob et al. 

Nature Methods. 2020-06-01. Vol. 17, num. 6, p. 629-635. DOI : 10.1038/s41592-020-0837-5.

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Patient‐specific logic models of signaling pathways from screenings on cancer biopsies to prioritize personalized combination therapies

F. Eduati; P. Jaaks; J. Wappler; T. Cramer; C. Merten et al. 

Molecular Systems Biology. 2020-02-19. Vol. 16, num. 2, p. e8664. DOI : 10.15252/msb.20188664.

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Antibiotics-induced monodominance of a novel gut bacterial order

F. Hildebrand; L. Moitinho-Silva; S. Blasche; M. T. Jahn; T. I. Gossmann et al. 

Gut. 2020-01-18. Vol. 68, num. 10, p. 1781-1790. DOI : 10.1136/gutjnl-2018-317715.

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2019

Journal Articles

Microfluidics as an Emerging Precision Tool in Developmental Biology

K. F. Sonnen; C. Merten 

Developmental Cell. 2019-02-11. Vol. 48, num. 3, p. 293-311. DOI : 10.1016/j.devcel.2019.01.015.

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Reviews

Microfluidics as an Enabling Technology for Personalized Cancer Therapy

L. Mathur; M. Ballinger; R. Utharala; C. Merten 

Small. 2019-11-20.  p. 1904321. DOI : 10.1002/smll.201904321.

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2018

Journal Articles

A microfluidics platform for combinatorial drug screening on cancer biopsies

F. Eduati; R. Utharala; D. Madhavan; U. P. Neumann; T. Longerich et al. 

Nature Communications. 2018-06-22. Vol. 9, num. 1, p. 2434. DOI : 10.1038/s41467-018-04919-w.

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A Versatile, Low-Cost, Multiway Microfluidic Sorter for Droplets, Cells, and Embryos

R. Utharala; Q. Tseng; E. E. M. Furlong; C. Merten 

Analytical Chemistry. 2018-04-24. Vol. 90, num. 10, p. 5982-5988. DOI : 10.1021/acs.analchem.7b04689.

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Modulation of Phase Shift between Wnt and Notch Signaling Oscillations Controls Mesoderm Segmentation

K. F. Sonnen; V. M. Lauschke; J. Uraji; H. J. Falk; Y. Petersen et al. 

Cell. 2018-02-22. Vol. 172, num. 5, p. 1079-1090.e12. DOI : 10.1016/j.cell.2018.01.026.

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Single-Cell Droplet Microfluidic Screening for Antibodies Specifically Binding to Target Cells

N. Shembekar; H. Hu; D. Eustace; C. Merten 

Cell Reports. 2018-02-20. Vol. 22, num. 8, p. 2206-2215. DOI : 10.1016/j.celrep.2018.01.071.

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2017

Journal Articles

Microfluidic single-cell technology in immunology and antibody screening

Y. F. S. Seah; H. Hu; C. Merten 

Molecular Aspects of Medicine. 2017-09-23. Vol. 59, p. 47-61. DOI : 10.1016/j.mam.2017.09.004.

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Single-Virus Droplet Microfluidics for High-Throughput Screening of Neutralizing Epitopes on HIV Particles

C. Chaipan; A. Pryszlak; H. Dean; P. Poignard; V. Benes et al. 

Cell Chemical Biology. 2017-05-25. Vol. 24, num. 6, p. 751-757.e3. DOI : 10.1016/j.chembiol.2017.05.009.

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Microfluidic train station: highly robust and multiplexable sorting of droplets on electric rails

D. Frenzel; C. Merten 

Lab on a Chip. 2017-02-17. Vol. 17, num. 6, p. 1024-1030. DOI : 10.1039/C6LC01544A.

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2016

Journal Articles

The International Human Epigenome Consortium: A Blueprint for Scientific Collaboration and Discovery

H. G. Stunnenberg; M. Hirst; S. Abrignani; D. Adams; M. de Almeida et al. 

Cell. 2016-11-17. Vol. 167, num. 5, p. 1145-1149. DOI : 10.1016/j.cell.2016.11.007.

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Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics

N. Shembekar; C. Chaipan; R. Utharala; C. Merten 

Lab on a Chip. 2016-03-18. Vol. 16, num. 8, p. 1314-1331. DOI : 10.1039/C6LC00249H.

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2015

Journal Articles

Quantifying Preferences and Responsiveness of Marine Zooplankton to Changing Environmental Conditions using Microfluidics

J. C. Molinero; N. Ramanathan; O. Simakov; C. Merten; D. Arendt 

PLoS One. 2015-10-30. Vol. 10, num. 10, p. e0140553. DOI : 10.1371/journal.pone.0140553.

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Efficient cell pairing in droplets using dual-color sorting

H. Hu; D. Eustace; C. A. Merten 

Lab on a Chip. 2015-08-17. Vol. 15, num. 20, p. 3989-3993. DOI : 10.1039/C5LC00686D.

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Dynein-Based Accumulation of Membranes Regulates Nuclear Expansion in Xenopus laevis Egg Extracts

Y. Hara; C. Merten 

Developmental Cell. 2015-05-21. Vol. 33, num. 5, p. 562-575. DOI : 10.1016/j.devcel.2015.04.016.

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Soft compartmentalization: Combining droplet-based microfluidics with freely accessible cells

D. Eicher; N. Ramanathan; C. Merten 

Engineering in Life Sciences. 2015-03-05. Vol. 15, num. 3, p. 297-305. DOI : 10.1002/elsc.201400184.

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2014

Journal Articles

Screening Europe 2010: an update about the latest technologies and applications in high-throughput screening

C. Merten 

Expert Review of Molecular Diagnostics. 2014-01-09. Vol. 10, num. 5, p. 559-563. DOI : 10.1586/erm.10.42.

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Reviews

Microfluidic devices for diagnostic applications

D. Eicher; C. Merten 

Expert Review of Molecular Diagnostics. 2014-01-09. Vol. 11, num. 5, p. 505-519. DOI : 10.1586/erm.11.25.

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2012

Journal Articles

Fragmentation of DNA in a sub-microliter microfluidic sonication device

Q. Tseng; A. M. Lomonosov; E. E. M. Furlong; C. Merten 

Lab on a Chip. 2012-09-26. Vol. 12, num. 22, p. 4677. DOI : 10.1039/c2lc40595d.

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Functional single-cell hybridoma screening using droplet-based microfluidics

B. E. Debs; R. Utharala; I. V. Balyasnikova; A. D. Griffiths; C. Merten 

Proceedings of the National Academy of Sciences. 2012-07-02. Vol. 109, num. 29, p. 11570-11575. DOI : 10.1073/pnas.1204514109.

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Reviews

Micro segmented-flow in biochemical and cell-based assays

J. Clausell-Tormos; C. Merten 

Frontiers in Bioscience. 2012-01-01. Vol. E4, num. 1, p. 1768. DOI : 10.2741/e497.

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2011

Journal Articles

A Competitive Co-cultivation Assay for Cancer Drug Specificity Evaluation

B. W. El Debs; U. Tschulena; A. D. Griffiths; C. Merten 

Journal of Biomolecular Screening. 2011-07-25. Vol. 16, num. 8, p. 818-824. DOI : 10.1177/1087057111414898.

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2010

Journal Articles

Miniaturization and Parallelization of Biological and Chemical Assays in Microfluidic Devices

S. Vyawahare; A. D. Griffiths; C. Merten 

Chemistry & Biology. 2010-10-28. Vol. 17, num. 10, p. 1052-1065. DOI : 10.1016/j.chembiol.2010.09.007.

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Coupling the Inhibition of Viral Transduction with a Positive Fluorescence Signal

J. Clausell-Tormos; C. Merten; A. D. Griffiths 

Combinatorial Chemistry & High Throughput Screening. 2010-05-01. Vol. 13, num. 4, p. 352-357. DOI : 10.2174/138620710791054312.

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High-Throughput Screening of Enzymes by Retroviral Display Using Droplet-Based Microfluidics

L. Granieri; J-C. Baret; A. D. Griffiths; C. Merten 

Chemistry & Biology. 2010-03-27. Vol. 17, num. 3, p. 229-235. DOI : 10.1016/j.chembiol.2010.02.011.

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An automated two-phase microfluidic system for kinetic analyses and the screening of compound libraries

J. Clausell-Tormos; A. D. Griffiths; C. Merten 

Lab on a Chip. 2010-03-03. Vol. 10, num. 10, p. 1302. DOI : 10.1039/b921754a.

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Nanoliter plates—versatile tools for the screening of split-and-mix libraries on-bead and off-bead

G. Upert; C. A. Merten; H. Wennemers 

Chemical Communications. 2010-02-23. Vol. 46, num. 13, p. 2209. DOI : 10.1039/b927017e.

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Reviews

Retroviral Display in Gene Therapy, Protein Engineering, and Vaccine Development

J. H. Urban; C. Merten 

ACS Chemical Biology. 2010-12-10. Vol. 6, num. 1, p. 61-74. DOI : 10.1021/cb100285n.

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2009

Journal Articles

A competition-based assay for the screening of species-specific antibiotics

L. Granieri; O. J. Miller; A. D. Griffiths; C. Merten 

Journal of Antimicrobial Chemotherapy. 2009-04-28. Vol. 64, num. 1, p. 62-68. DOI : 10.1093/jac/dkp137.

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Imaging of mRNA in Live Cells Using Nucleic Acid-Templated Reduction of Azidorhodamine Probes

Z. Pianowski; K. Gorska; L. Oswald; C. Merten; N. Winssinger 

Journal of the American Chemical Society. 2009-04-20. Vol. 131, num. 18, p. 6492-6497. DOI : 10.1021/ja809656k.

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2008

Journal Articles

Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms

J. Clausell-Tormos; D. Lieber; J-C. Baret; A. El-Harrak; O. J. Miller et al. 

Chemistry & Biology. 2008-08-25. Vol. 15, num. 5, p. 427-437. DOI : 10.1016/j.chembiol.2008.04.004.

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Drop-based microfluidic devices for encapsulation of single cells

S. Köster; F. E. Angilè; H. Duan; J. J. Agresti; A. Wintner et al. 

Lab on a Chip. 2008-05-23. Vol. 8, num. 7, p. 1110. DOI : 10.1039/b802941e.

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2006

Journal Articles

Fusoselect: cell-cell fusion activity engineered by directed evolution of a retroviral glycoprotein

C. Merten; J. Stitz; G. Braun; J. Medvedovska; K. Cichutek et al. 

Nucleic Acids Research. 2006-03-01. Vol. 34, num. 5, p. e41-e41. DOI : 10.1093/nar/gkl053.

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2005

Journal Articles

Circumventing Tolerance to the Prion Protein (PrP): Vaccination with PrP-Displaying Retrovirus Particles Induces Humoral Immune Responses against the Native Form of Cellular PrP

D. Nikles; P. Bach; K. Boller; C. Merten; F. Montrasio et al. 

Journal of Virology. 2005-03-14. Vol. 79, num. 7, p. 4033-4042. DOI : 10.1128/JVI.79.7.4033-4042.2005.

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2004

Journal Articles

Directed Evolution of Retrovirus Envelope Protein Cytoplasmic Tails Guided by Functional Incorporation into Lentivirus Particles

C. Merten; J. Stitz; G. Braun; E. M. Poeschla; K. Cichutek et al. 

Journal of Virology. 2004-12-20. Vol. 79, num. 2, p. 834-840. DOI : 10.1128/JVI.79.2.834-840.2005.

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Induction of Prion Protein-Specific Antibody Responses Using PrP-Retroparticles

D. Nikles; P. Bach; K. Boller; C. Merten; F. Montrasio et al. 

Molecular Therapy. 2004-05-01. Vol. 9, p. S211. DOI : 10.1016/j.ymthe.2004.06.486.

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Vector Development by Molecular Evolution: A Selection Procedure for Envelope Proteins Pseudotyping Lentiviral Particles

C. Merten; J. Stitz; K. Cichutek; C. J. Buchholz 

Molecular Therapy. 2004-05-01. Vol. 9, p. S126. DOI : 10.1016/j.ymthe.2004.06.275.

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2003

Journal Articles

Displaying Epidermal Growth Factor on Spleen Necrosis Virus-Derived Targeting Vectors

C. Merten; M. Engelstaedter; C. J. Buchholz; K. Cichutek 

Virology. 2003-01-05. Vol. 305, num. 1, p. 106-114. DOI : 10.1006/viro.2002.1778.

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