Our laboratory is interested in understanding how temporal fluctuations in gene expression and the interplay between transcription factors and the cell cycle impact cell fate decisions. To tackle these problems, we develop quantitative methods to characterize the dynamics of gene expression in individual cells based on luminescent/fluorescent reporters and gene editing. We use genomics approaches (ChIP-seq, ATAC-seq, etc.) to determine how gene regulation is impacted in different cell cycle phases.
From top to bottom:
1. Two-color bioluminescence imaging allowing absolute quantifications of the levels of the Sox2 transcription factor in living cells (red), which predict the probability of embryonic stem cells differentiate into neuroectoderm (cytoplasmic green signal). Green nuclei: cells used to calibrate red and green signals (Strebinger et al., bioRxiv 2018)
2. A fluorescent timer fused to the endogenous laminB1 protein allows to measure changes in protein synthesis and degradation over the cell cycle (Alber et al., Molecular Cell 2018)
3. Sox2 (yellow) cellular distribution during the cell cycle illustrating its association to chromosomes during mitosis (Deluz et al., Genes & Development 2016)
4. Ultrasensitive bioluminescence live cell imaging allowing to record transcriptional memory of an endogenous gene (Phillips et al., Nature Communications 2019)
We are currently offering a joint Master project (at least 6 months) aiming at studying proteostasis in the Zebrafish. The project will involve various techniques such as DNA cloning, zebrafish transgenesis and multicolor fluorescence imaging. The project is to be carried out jointly with the laboratory of Andy Oates (https://www.epfl.ch/labs/oateslab/). Prospective Post-doctoral researchers, PhD and Master students are encouraged to apply by directly writing do [email protected]