Laboratory of Stem Cell Bioengineering

About us

By interfacing advanced bioengineering strategies and stem cell biology, the overarching goal of the Lutolf Laboratory is to uncover mechanisms of stem cell fate regulation; knowledge that will contribute to better ways to grow stem cells in culture and use them for various applications. A major current focus of the lab is on coaxing stem cells in vitro into miniature, organ-mimicking constructs, termed ‘organoids’, by exposing them to well-controlled artificial signaling microenvironments. The lab’s ambition is to achieve organoid development in a more reproducible and ultimately predictable manner by ‘guiding’ stem cell-based self-organization. Beyond contributing to a better understanding of key principles of multicellular self-organization, this research should open up exciting prospects for drug discovery and regenerative medicine.

Selected publications

Gjorevski, N., Sachs, N., Manfrin, A., Giger, S., Bragina, M.E., Ordonez-Moran, P., Clevers, H., Lutolf, M.P.*, Designer matrices for intestinal stem cell and organoid culture, Nature, 539, 560-564 (2016)

Ranga, A., Girgin, M., Meinhardt, A., Eberle, D., Caiazzo, M., Tanaka, E.M., Lutolf, M.P.*, Neural tube morphogenesis in synthetic 3D microenvironments, PNAS, E6831–E6839, doi: 10.1073/pnas.1603529113 doi: 10.1073/pnas.1603529113 (2016)

Vannini, N., Girotra, M., Naveiras, O., Nikitin, G., Campos, V., Giger, S., Roch, A., Auwerx, J., Lutolf, M.P.*, Specification of haematopoietic stem cell fate via modulation of mitochondrial activity, Nature Communications, 7. 13125. doi:10.1038/ncomms13125 (2016)

Brandenberg, N., Lutolf, M.P.*, In situ patterning of microfluidic networks in 3D cell-laden hydrogels, Advanced Materials, 28(34):7450-6. doi: 10.1002/adma.201601099 (2016)

Caiazzo, M., Okawa, Y., Ranga, A., Piersigilli, A., Tabata,Y., Lutolf, M.P.*, Defined three-dimensional microenvironments boost induction of pluripotency, Nature Materials, 15, 344–352 (2016)

Gjorevski, N.*, Ranga, A., Lutolf, M.P.*, Bioengineering approaches to guide stem cell-based organogenesis, Development 141 (9), 1794-1804 (2014)

Ranga, A., Gobaa, S., Mosiewicz, K.A., Okawa, Y., Negro, A., Lutolf, M.P.*, 3D niche microarrays for systems-level analyses of cell fate, Nature Communications, 5, 4324 (2014)

Mosiewicz, K.A., Kolb, L., van der Vlies, A.J., Martino, M.M., Lienemann, P.S., Hubbell, J.A., Ehrbar, M., Lutolf, M.P.*, In situ cell manipulation through enzymatic hydrogel photopatterning, Nature Materials, 12 (11), 1072-1078 (2014)

Roccio, M., Schmitter, D., Knobloch, M., Okawa, Y., Sage, D., and Lutolf M.P.*, Predicting stem cell fate changes by differential cell cycle progression patterns, Development, 140, 459-470 (2013)

Gobaa, S., Hoehnel, S., Roccio, M., Negro, A., Kobel, S., and Lutolf M.P.*, Artificial niche microarrays for probing single stem cell fate in high throughput, Nature Methods, 8(11):949-55 (2011)

Gilbert, P.M., Havenstrite, K.L., Magnusson, K.E.G., Sacco, A., Leonardi, N.A., Kraft, P., Nguyen, N.K., Thrun, S., Lutolf, M.P. and Blau, H.M.*, Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture, Science, 329(5995):1078-1081 (2010)

Lutolf, M.P.*, Gilbert, P.M., and Blau, H.M.*, Designing materials to direct stem-cell fate, Nature, 462, 433-441 (2009)

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