New techniques for high-throughput glycan analysis

This aim of this project is to develop an high-throughput analytical method to identify glycans from biological samples. The challenge of doing so arises from the isomeric complexity of glycans, which is difficult to resolve by any single analytical tool. Our approach combines ultrahigh resolution ion mobility based on structures for lossless ion manipulations (SLIM) with cryogenic ion spectroscopy and mass spectrometry to perform glycan analysis at high throughput.  

See some of our recent papers for details:

Separation and Identification of Glycan Anomers Using Ultrahigh-Resolution Ion Mobility Spectrometry and Cryogenic Ion Spectroscopy

S. Warnke; A. Ben Faleh; V. Scutelnic; T. R. Rizzo 

Journal of the American Society for Mass Spectrometry. 2019. DOI : 10.1007/s13361-019-02333-0.

Combining ultra-high resolution ion-mobility spectrometry with cryogenic IR spectroscopy for the study of biomolecular ions

S. Warnke; A. Ben Faleh; R. P. Pellegrinelli; N. Yalovenko; T. R. Rizzo 

Faraday Discussions. 2019. DOI : 10.1039/C8FD00180D.

Combining Ultrahigh-Resolution Ion-Mobility Spectrometry with Cryogenic Infrared Spectroscopy for the Analysis of Glycan Mixtures

A. Ben Faleh; S. Warnke; T. R. Rizzo 

Analytical Chemistry. 2019. DOI : 10.1021/acs.analchem.9b00659.
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