This aim of this project is to develop an high-throughput analytical method to identify glycans from biological samples. The challenge of doing so arises from the isomeric complexity of glycans, which is difficult to resolve by any single analytical tool. Our approach combines ultrahigh resolution ion mobility based on structures for lossless ion manipulations (SLIM) with cryogenic ion spectroscopy and mass spectrometry to perform glycan analysis at high throughput.
See some of our recent papers for details:
Device, system, and method for ion fragmentation by use of an ion mobility device and messenger tagging
Identification of Mobility-Resolved N-Glycan IsomersAnalytical Chemistry. 2022-07-07. Vol. 94, num. 28, p. 10101-10108. DOI : 10.1021/acs.analchem.2c01181.
A New Approach for Identifying Positional Isomers of Glycans Cleaved from Monoclonal AntibodiesChimia. 2022-04-01. Vol. 76, num. 4, p. 363-363. DOI : 10.2533/chimia.2022.363.
High-Throughput Multiplexed Infrared Spectroscopy of Ion Mobility-Separated Species Using Hadamard TransformAnalytical Chemistry. 2022-02-03. Vol. 94, num. 6, p. 2912–2917. DOI : 10.1021/acs.analchem.1c04843.
Identification of N-glycan positional isomers by combining IMS and vibrational fingerprinting of structurally determinant CID fragmentsAnalyst. 2022-01-21. DOI : 10.1039/d1an01861b.
A method and system to build an ir fingerprint database for the structural identification of biomolecules
Combining ultra-high resolution ion mobility with cryogenic ion vibrational spectroscopy for the analysis of glycans2019-03-31. National Meeting of the American-Chemical-Society (ACS), Orlando, FL, Mar 31-Apr 04, 2019.
High-throughput cryogenic spectroscopy for glycan analysis