What we produce:
For both AAV and lentiviral vectors, we can provide or adapt various constructs for (1) gene overexpression in a cell type specific manner, (2) gene silencing by RNA interference or (3) gene editing.
What we provide:
1- Vector design and/or cloning
If the construct is already available, we proceed with vector packaging. In addition, we can adapt any construct with your sequence(s) of interest and/or perform the full design and cloning of the transfer construct. We have a broad collection of available vector backbones (AAVs and lentivirus) containing a variety of promoters, reporter genes and enhancers.
2- Vector production
For AAV, our platform has developed various technologies to improve the yield and the quality of vector production. We currently obtain vector batches at high titer and purity using transient transfection of mammalian cells (HEK293) grown in orbital-shaken suspension cultures. Our purification procedures are mostly based on affinity or ion exchange chromatography methods.
For production of lentivirus, we use second and third generation systems based on transient co-transfection of adherent HEK293T cells. High-titer vector stocks compatible with CNS applications can be obtained by ultracentrifugation.
3- Vector titration
For AAV titration, our platform has established a reliable procedure to quantify the concentration of genome-containing viral particles using digital PCR (dPCR). This method allows for the absolute quantification of encapsidated viral genomes present in the final vector suspension.
For lentivirus the viral titers are determined by p24 antigen measurements, or/and qPCR-based infectivity testing.
4- In vivo application
We can advise on methods and support the administration of vectors in rodent models.
How to proceed?
Please contact us for any question and/or send the completed order form :
Order form :