Super-resolution microscopy builds multicolor 3D from 2D

A new technique overcomes the noise and color limitations of super-resolution microscopy by creating three-dimensional reconstructions from single-color, two-dimensional images of protein complexes.

Image: Human centrioles labelled with antibodies against two proteins (Cep152, HsSAS-6) and imaged using super-resolution microscopy.

Christian Sieben, Niccolò Banterle, Kyle M. Douglass, Pierre Gönczy, Suliana Manley. Multicolor single-particle reconstruction of protein complexes. Nature Methods, 2018; 15 (10): 777 doi:10.1038/s41592-018-0140-x

Source: EPFL News