Simanis lab – regulation of the cell cycle in mitosis and meiosis
Accuracy matters during the cell cycle; S-phase, mitosis and cytokinesis must be executed in the correct order, with high fidelity. Failure to do this may cause a cell’s death or alter its behaviour. An imprecisely executed meiosis may produce inviable gametes, or alter their ploidy. We study the spatial and temporal coordination of the cell cycle in meiosis and mitosis, using the fission yeast Schizosaccharomyces pombe as a model.
the “standard model” for SIN signalling (see below and Simanis (2015) for details).
coordination of cytokinesis with DNA synthesis and mitosis is essential. Ectopic activation of SIN signalling can trigger cytokinesis in interphase (bottom cell) or prematurely in mitosis (top cell), cutting the incompletely segregated chromosomes.
loss of Dma1p function in meiosis affects spore formation. Wildtype asci contain four spores; by contrast, without dma1-D function, diploids still produce four nuclei at the end of meiosis, but then fail to form a spore around one of more of them in more than 75% of asci.