Category: line1
New Paper – Highly frequent undesired insertional mutagenesis during Drosophila genome editing
CRISPR/Cas9 based genome editing employing Homology Directed Repair (HDR) from template vector sequences is a widely used technique to enable precise insertions, deletions or modifications to genes. Here, we describe an undesired and highly frequent editing event when using conventional CRISPR/Cas9 plus HDR methods for Drosophila melanogaster germline genome editing. We find that the template (…)
New preprint – Spontaneous neurotransmission is regulated!
New manuscript – Spontaneous neurotransmitter release is regulated by Unc-5 For decades, spontaneous neurotransmitter vesicle release (also known as miniature neurotransmission or ‘minis’), which occurs at every chemical synapse in the brain, was considered little more than synaptic noise — a background hum without meaningful function. Our lab has spent years showing that’s wrong. Minis (…)
pBID2 vectors including a new QF/Gal4 hybrid binary system released
Binary gene regulatory expression systems are a linchpin technology critical for genetic manipulations of many model organisms including in Drosophila. In a new manuscript, Evelyne has generated a standardized & optimized series of vectors (pBID2) that support the parallel construction of Drosophila enhancer-driven ‘activator’ Gal4, LexA, or QF2 lines, along with matched vectors for generating (…)