The lab is interested in leveraging DNA nanotechnology to design, test, and apply smart probes that push the boundaries of fluorescence microscopy in terms of spatial resolution, throughput, quantitative imaging, and single nanometer proximity mapping (molecular connectomics). Furthermore, we aim to advance both conventional and super-resolution multiplexed imaging (DNA-PAINT, FLASH-PAINT) toward imaging-based spatial omics. We are interested in applying the technology to microbiology and microbiome research, but our focus is not limited to these areas.
Research Projects
- Schueder F, et al. Multiplexed 3D super-resolution imaging of whole cells using spinning disk confocal microscopy and DNA-PAINT. Nat Commun. 2017
- Schueder F, et al. An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions. Nat Methods. 2019
- Schueder F, et al. Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging. Cell. 2024
- Schueder F, et al. Universal Super-Resolution Multiplexing by DNA Exchange. Angew Chem Int Ed Engl. 2017
- Schueder F, Bewersdorf J. Omics goes spatial epigenomics. Cell. 2022
- Schueder F, et al. Super-Resolution Spatial Proximity Detection with Proximity-PAINT. Angew Chem Int Ed Engl. 2021
- Schueder et al. Nanobodies combined with DNA-PAINT super-resolution reveal a staggered titin nanoarchitecture in flight muscles. eLife 2023
- Schlichthaerle T et al. Direct Visualization of Single Nuclear Pore Complex Proteins Using Genetically-Encoded Probes for DNA-PAINT. Angew Chem Int Ed Engl. 2019
- Strauss, M.T. et al. Quantifying absolute addressability in DNA origami with molecular resolution. Nat Commun 2018