Partner: École Polytechnique Fédérale de Lausanne; Center of Studies on Air quality and Climate Change, ICEHT- FORTH
PI: Prof. Nenes Athanasios ([email protected])
Operator: Carolina Molina ([email protected])
The oxidative potential (OP) of aerosols is a metric that represents the adverse health effect of particles related to oxidative stress. The Dithiothreitol (DTT) and Ascorbic acid (AA) assays are widely used methods for estimating aerosol OP. We employ two semi-automated system previously described by Fang et al., (2015). The OP is expressed as the rate of consumption of these compounds over time. Briefly, aerosol extracts and chemicals are transferred using programmable syringe pumps (Norgren Ltd, UK). The mixtures are incubated and constantly shaken with potassium phosphate buffer along with DTT or AA (Thermomixer, Eppendorf, NY, USA). Every 10 minutes, an aliquot of the mixture is taken and its absorbance is measured using a liquid waveguide capillary cell (LWCC, 10 cm, World of Precision, UK) connected to an online spectrophotometer (Light source: DH mini, detector: model Flame, OceanOptics, FL, USA). Additionally, we have a cuvette-bassed UV-VIS spectrophotometer (Hitachi, model U-3010), where we can perform the manual protocols for these assays.
