Zeiss LSM 700 Upright Microscope.
Multi-purpose confocal with tPMT, widefield & brightfield capability. Manual stage.
Person in charge: José Artacho & Thierry Laroche
Book on SV-BBS
Technical Specifications
Imaging: Confocal & Widefield
Stand: Uproght Zeiss AxioObserver Z1
XY-Stage: Motorized
Illumination: HXP-120 Metal Halide Lamp
Software: ZEN 2009
Detectors
2 photomultiplier tubes (PMT) for fluorescence.
1 transmission photomultiplier tube (PMT) for brightfield.
| Camera | Axiocam MRm (B/W) |
| Chip Technology | CCD Grayscale |
| Resolution | 1388 x 1040 |
| Chip size | 8.0 mm x 6.7 mm |
| Pixel size | 6.45 um x 6.45 um |
| Dynamic Range | 12-bit |
| C-mount | 1.0x |
Objectives
| Objective | Mag/NA | Medium | Contrast | WD (mm) |
| EC Plan-Neofluar | 10x/0.30 | air | DIC | 5.20 |
| Plan-Apochromat | 20x/0.80 | air | DIC | 0.55 |
| Plan-Apochromat | 40x/1.30 | oil | DIC | 0.21 |
| Plan-Apochromat | 63x/1.40 | oil | DIC | 0.19 |
| N-Achromat | 20x/0.50 | water | – | 2.60 |
| N-Achromat | 63x/0.80 | water | – | 2.40 |
Laser Lines
| Wavelength | Laser Type |
| 405 nm | Solid state |
| 488 nm | Solid state |
| 555 nm | Solid state |
| 639 nm | Solid state |
Filtercubes
The links in the table below will send you to an FPbase configuration page.
| Name | Excitation | Dichroic | Emission | No |
| Blue | G 365 | FT 395 | BP 445/50 | FS 49 |
| Green | BP 450-490 | FT 510 | BP 515-565 | FS 10 |
| Red | BP 546/12 | FT 560 | BP 575-640 | FS 20 |
| Far-Red | BP 640/30 | FT 660 | BP 690/50 | FS 50 |