Student Projects

Timeframe

Master Thesis (5-6 months full-time), Internship (2-6 months full-time) or Semester Project (2-4 months part-time)

Contact

Yang LIU ([email protected])

Description

For aerobic bacteria, the oxygen consumption is an indicator of their viability. Under the exposure of antimicrobials, bacteria might be killed or inactive and consume no more oxygen.

The oxygen concentration measurement is based on a nanoparticle from a collaborator, for which the fluorescent signal is quenched in the presence of oxygen. Thus, a correlation between the concentration of oxygen and the fluorescent signal is established.

This project consists of

1. To realize of a microfluidic chip to conduct the oxygen concentration measurement by 3D printing or photolithography.

2. To find an appropriate way to sterilize the chip in order to reuse the nanoparticle spot

3. To perform series of experiments with antimicrobials.

4. To construct a data processing program with MATLAB.

Some preliminary experiments to validate of the working principle have been done.

Timeframe

Master Thesis (5-6 months full-time), Internship (2-6 months full-time) or Semester Project (2-4 months part-time)

Contact

Yang LIU ([email protected])

Description

Bacterial motility is an important indicator of the bacterial viability. It can be studied with time-lapse imaging.

This project aims to study the antimicrobial effects on bacterial motility. A microfluidic chip with mixing module and observation chamber was designed. Firstly, student will be able to do image acquisition of the swimming bacteria with digital microscopy or optical microscopy (to be discussed). To promote bacterial movement on the surface, surface modification will need to be performed. Then Image processing involves extracting the coordinates of bacteria from the image, bacterial trajectories and extract statistical information such as velocities and bacterial quantity.

Knowledge with MATLAB programming is required; photolithography knowledge and microbiological background will be an advantage.

Main tasks:

• PDMS or UV adhesive chip fabrication and surface modification

• Time lapse imaging with microscopy

Timeframe

Master Thesis (5-6 months full-time), Internship (2-6 months full-time) or Semester Project (2-4 months part-time)

Contact

Fabien Tâche ([email protected])

Description

The Laboratory of Microsystems (LMIS2) is developing an innovative laboratory device for drug discovery research. The technology employs robotics and computer vision for the automated culture and analysis of micro-organisms within microfluidic chips, as powerful biological models to investigate human diseases and test their potential cures. In this framework, we look for students to contribute to the development of the following microfluidics components:

• Highly-integrated distribution valves with a high number of outputs

• High resolution syringe pumps

• New generation of microfluidic chips, including the optimization of the manufacturing process

The aim of this project is to design one (or several) of the devices listed above, manufacture them, and characterize their performances. If the implementation is successful, the devices will be integrated in the existing robotic platform and used for experiments on C. elegans.

The overall project holds great potential to represent a breakthrough in biomedical research and, as such, resides at the core of a startup project (Nagi Bioscience).

Timeframe

Master Thesis (5-6 months full-time), Internship (2-6 months full-time) or Semester Project (2-4 months part-time)

Contact

Fabien Tâche ([email protected])

Description

The Laboratory of Microsystems (LMIS2) is developing an innovative laboratory device for drug discovery research. The technology employs robotics and computer vision for the automated culture and analysis of micro-organisms within microfluidic chips, as powerful biological models to investigate human diseases and test their potential cures. In this framework, we look for students to contribute to the development of experimental protocols and run biological assays. This includes the following tasks:

• Definition of experimental protocols

• Implementation and optimization of protocols (manipulation of C. elegans using microfluidics, feeding of worms, control of worm chamber temperature, …)

• Perform biological assays to validate the technology and protocols

This project is highly multidisciplinary, because it is at the interface of technology, life sciences and entrepreneurship.

The overall project holds great potential to represent a breakthrough in biomedical research and, as such, resides at the core of a startup project (Nagi Bioscience).

Timeframe

Master Thesis (5-6 months full-time), Internship (2-6 months full-time) or Semester Project (2-4 months part-time)

Contact

Fabien Tâche ([email protected])

Description

The Laboratory of Microsystems (LMIS2) is developing an innovative laboratory device for drug discovery research. The technology employs robotics and computer vision for the automated culture and analysis of micro-organisms within microfluidic chips, as powerful biological models to investigate human diseases and test their potential cures. In this framework, we look for students to contribute to the development of a tracking and analysis software. This project includes the following tasks:

• Implementation of worm tracking algorithms

• Implementation of a worm phenotype analysis software

• Characterization, optimization and validation of algorithms

• Implementation of a graphical user interface (GUI) displaying the results of automated analysis

This project requires good software development and image processing skills. Machine learning knowledge is a plus.

The overall project holds great potential to represent a breakthrough in biomedical research and, as such, resides at the core of a startup project (Nagi Bioscience).