Publications

Journal Articles

2018

Unintended specificity of an engineered ligand-binding protein facilitated by unpredicted plasticity of the protein fold

A. L. Day; P. Greisen; L. Doyle; A. Schena; N. Stella et al. 

Protein Engineering Design & Selection. 2018-10-01. Vol. 31, num. 10, p. 375-387. DOI : 10.1093/protein/gzy031.

Semisynthetic sensor proteins enable metabolic assays at the point of care

Q. Yu; L. Xue; J. Hiblot; R. Griss; S. Fabritz et al. 

Science. 2018-09-14. Vol. 361, num. 6407, p. 1122-1125. DOI : 10.1126/science.aat7992.

SNAP-Tagged Nanobodies Enable Reversible Optical Control of a G Protein-Coupled Receptor via a Remotely Tethered Photoswitchable Ligand

H. Farrants; V. A. Gutzeit; A. Acosta-Ruiz; D. Trauner; K. Johnsson et al. 

Acs Chemical Biology. 2018-09-01. Vol. 13, num. 9, p. 2682-2688. DOI : 10.1021/acschembio.8b00628.

Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides

O. Sallin; L. Reymond; C. Gondrand; F. Raith; B. Koch et al. 

eLife. 2018-05-29. Vol. 7. DOI : 10.7554/eLife.32638.

Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor (vol 8, 1862, 2017)

M. Berry; A. Holt; J. Levitz; J. Broichhagen; B. Gaub et al. 

Nature Communications. 2018. Vol. 9. DOI : 10.1038/s41467-018-03585-2.

Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons

R. Dhandapani; C. Arokiaraj; F. Taberner; P. Pacifico; S. Raja et al. 

Nature Communications. 2018. Vol. 9. DOI : 10.1038/s41467-018-04049-3.

A New Fluorogenic Small-Molecule Labeling Tool for Surface Diffusion Analysis and Advanced Fluorescence Imaging of beta-site Amyloid Precursor Protein-Cleaving Enzyme 1 Based on Silicone Rhodamine: SiR-BACE1

S. Karch; J. Broichhagen; J. Schneider; D. Boning; S. Hartmann et al. 

JOURNAL OF MEDICINAL CHEMISTRY. 2018. Vol. 61, num. 14, p. 6121-6139. DOI : 10.1021/acs.jmedchem.8b00387.

2017

Sampling and Energy Evaluation Challenges in Ligand Binding Protein Design

J. Dou; L. Doyle; P. J. Greisen; A. Schena; H. Park et al. 

Protein Science. 2017. Vol. 26, num. 12, p. 2426-2437. DOI : 10.1002/pro.3317.

Computational design of environmental sensors for the potent opioid fentanyl

M. J. Bick; P. J. Greisen; K. J. Morey; M. S. Antunes; D. La et al. 

eLife. 2017. Vol. 6. DOI : 10.7554/eLife.28909.

Luciferases with tunable emission wavelengths

K. Johnsson; J. Hiblot; Q. Yu; M. Sabbadini; L. Reymond et al. 

Angewandte Chemie International Edition. 2017. Vol. 56, num. 46, p. 14556-14560. DOI : 10.1002/anie.201708277.

Potent Prearranged Positive Allosteric Modulators of the Glucagon-like Peptide-1 Receptor

B. J. Jones; R. Scopelliti; A. Tomas; S. R. Bloom; D. J. Hodson et al. 

Chemistryopen. 2017. Vol. 6, num. 4, p. 501-505. DOI : 10.1002/open.201700062.

Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins

S. Scarabelli; K. T. Tan; R. Griss; R. Hovius; P. L. D’Alessandro et al. 

ACS Sensors. 2017. Vol. 2, num. 8, p. 1191-1197. DOI : 10.1021/acssensors.7b00331.

Dual optical control and mechanistic insights into photoswitchable group II and III metabotropic glutamate receptors

J. Levitz; J. Broichhagen; P. Leippe; D. Konrad; D. Trauner et al. 

Proceedings Of The National Academy Of Sciences Of The United States Of America. 2017. Vol. 114, num. 17, p. E3546-E3554. DOI : 10.1073/pnas.1619652114.

Expression proteomics study to determine metallodrug targets and optimal drug combinations

R. F. S. Lee; A. Chernobrovkin; D. Rutishauser; C. S. Allardyce; D. Hacker et al. 

Scientific Reports. 2017. Vol. 7, p. 1590. DOI : 10.1038/s41598-017-01643-1.

Bioluminescent Antibodies for Point-of-Care Diagnostics

L. Xue; Q. Yu; R. Griss; A. Schena; K. Johnsson 

Angewandte Chemie International Edition. 2017. Vol. 56, num. 25, p. 7112-7116. DOI : 10.1002/anie.201702403.

Identification of aminopyrimidine-sulfonamides as potent modulators of Wag31-mediated cell elongation in mycobacteria

V. Singh; N. Dhar; J. Pato; G. S. Kolly; J. Kordulakova et al. 

Molecular Microbiology. 2017. Vol. 103, num. 1, p. 13-25. DOI : 10.1111/mmi.13535.

2016

Acetylated tubulin is essential for touch sensation in mice

S. J. Morley; Y. Qi; L. Iovino; L. Andolfi; D. Guo et al. 

eLife. 2016. Vol. 5, p. 5:e20813. DOI : 10.7554/eLife.20813.

Chemical Genetic Screen Identifies Natural Products that Modulate Centriole Number

M. Graciotti; Z. Fang; K. Johnsson; P. Gönczy 

ChemBioChem. 2016. Vol. 17, num. 21, p. 2063-2074. DOI : 10.1002/cbic.201600327.

Fluorogenic Probes for Multicolor Imaging in Living Cells

G. Lukinavičius; L. Reymond; K. Umezawa; O. Sallin; E. D’Este et al. 

Journal of the American Chemical Society. 2016. Vol. 138, num. 30, p. 9365-9368. DOI : 10.1021/jacs.6b04782.

A General Strategy for the Semisynthesis of Ratiometric Fluorescent Sensor Proteins with Increased Dynamic Range

L. Xue; E. Prifti; K. Johnsson 

Journal of the American Chemical Society. 2016. Vol. 138, num. 16, p. 5258-5261. DOI : 10.1021/jacs.6b03034.

2015

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro

R. F. S. Lee; S. Escrig; M. Croisier; S. Clerc-Rosset; G. W. Knott et al. 

Chemical Communications. 2015. Vol. 51, num. 92, p. 16486-16489. DOI : 10.1039/c5cc06983a.

Tetrahydrobiopterin Biosynthesis as a Potential Target of the Kynurenine Pathway Metabolite Xanthurenic Acid

H. Haruki; R. Hovius; M. G. Pedersen; K. Johnsson 

Journal Of Biological Chemistry. 2015. Vol. 291, num. 2, p. 652-657. DOI : 10.1074/jbc.C115.680488.

Imaging and manipulating proteins in live cells through covalent labeling

L. Xue; I. A. Karpenko; J. Hiblot; K. Johnsson 

Nature Chemical Biology. 2015. Vol. 11, num. 12, p. 917-923. DOI : 10.1038/nchembio.1959.

SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy

G. Lukinavičius; C. Blaukopf; E. Pershagen; A. Schena; L. Reymond et al. 

Nature Communications. 2015. Vol. 6, p. 8497. DOI : 10.1038/ncomms9497.

Site-Specific Glycoconjugation of Protein via Bioorthogonal Tetrazine Cycloaddition with a Genetically Encoded trans-Cyclooctene or Bicyclononyne

T. Machida; K. Lang; L. Xue; J. W. Chin; N. Winssinger 

Bioconjugate Chemistry. 2015. Vol. 26, num. 5, p. 802-806. DOI : 10.1021/acs.bioconjchem.5b00101.

Modulating protein activity using tethered ligands with mutually exclusive binding sites

A. Schena; R. Griss; K. Johnsson 

Nature Communications. 2015. Vol. 6, p. 7830. DOI : 10.1038/ncomms8830.

Reduction of Neuropathic and Inflammatory Pain through Inhibition of the Tetrahydrobiopterin Pathway

A. Latremoliere; A. Latini; N. Andrews; S. Cronin; M. Fujita et al. 

Neuron. 2015. Vol. 86, num. 6, p. 1393-1406. DOI : 10.1016/j.neuron.2015.05.033.

Genetic targeting of chemical indicators in vivo

G. Yang; F. D. C. Reis; M. Sundukova; S. Pimpinella; A. Asaro et al. 

Nature Methods. 2015. Vol. 12, num. 2, p. 137-139. DOI : 10.1038/Nmeth.3207.

2014

FLUORESCENCE MICROSCOPY Strategic blinking

G. Lukinavicius; K. Johnsson 

Nature Chemistry. 2014. Vol. 6, num. 8, p. 663-664. DOI : 10.1038/nchem.2020.

Bioluminescent sensor proteins for point-of-care therapeutic drug monitoring

R. Griss; A. Schena; L. Reymond; L. Patiny; D. Werner et al. 

Nature Chemical Biology. 2014. Vol. 10, p. E2510-E2517. DOI : 10.1038/nchembio.1554.

Fluorogenic probes for live-cell imaging of the cytoskeleton

G. Lukinavičius; L. Reymond; E. D’Este; A. Masharina; F. Göttfert et al. 

Nature Methods. 2014. Vol. 11, p. 731-733. DOI : 10.1038/nmeth.2972.

The Efficacy of Raf Kinase Recruitment to the GTPase H-ras Depends on H-ras Membrane Conformer-specific Nanoclustering

C. Guzman; M. Solman; A. Ligabue; O. Blazevits; D. M. Andrade et al. 

Journal Of Biological Chemistry. 2014. Vol. 289, num. 14, p. 9519-9533. DOI : 10.1074/jbc.M113.537001.

A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red

E. Prifti; L. Reymond; M. Umebayashi; R. Hovius; H. Riezman et al. 

ACS Chemical Biology. 2014.  p. 140114114742001. DOI : 10.1021/cb400819c.

2013

Yeast Three-Hybrid Screening for Identifying Anti-Tuberculosis Drug Targets

S. Moser; K. Johnsson 

Chembiochem. 2013. Vol. 14, num. 17, p. 2239-2242. DOI : 10.1002/cbic.201300472.

Sensing Acetylcholine and Anticholinesterase Compounds

A. Schena; K. Johnsson 

Angewandte Chemie-International Edition. 2013. Vol. 53, num. 5, p. 1302-1305. DOI : 10.1002/anie.201307754.

Intramolecular Distances and Dynamics from the Combined Photon Statistics of Single-Molecule FRET and Photoinduced Electron Transfer

D. Haenni; F. Zosel; L. Reymond; D. Nettels; B. Schuler 

Journal Of Physical Chemistry B. 2013. Vol. 117, num. 42, p. 13015-13028. DOI : 10.1021/jp402352s.

Identification of a small molecule with activity against drug-resistant and persistent tuberculosis (vol 110, pg E2510, 2013)

F. Wang; D. Sambandan; R. Halder; J. Wang; S. M. Batt et al. 

Proceedings Of The National Academy Of Sciences Of The United States Of America. 2013. Vol. 110, num. 39, p. 15848-15848. DOI : 10.1073/pnas.1315914110.

Substrates for Improved Live-Cell Fluorescence Labeling of SNAP-tag

I. R. J. Correa; B. Baker; A. Zhang; L. Sun; C. R. Provost et al. 

Current Pharmaceutical Design. 2013. Vol. 19, num. 30, p. 5414-5420.

Commercial Cdk1 antibodies recognize the centrosomal protein Cep152

G. Lukinavičius; D. Lavogina; P. Gönczy; K. Johnsson 

Biotechniques. 2013. Vol. 55, num. 3, p. 111-114. DOI : 10.2144/000114074.

Computational design of ligand-binding proteins with high affinity and selectivity

C. E. Tinberg; S. D. Khare; J. Dou; L. Doyle; J. W. Nelson et al. 

Nature. 2013. Vol. 501, num. 7466, p. 212-6. DOI : 10.1038/nature12443.

Identification of a small molecule with activity against drug-resistant and persistent tuberculosis

F. Wang; D. Sambandan; R. Halder; J. Wang; S. M. Batt et al. 

Proceedings of the National Academy of Sciences. 2013. Vol. 110, num. 27, p. E2510-E2517. DOI : 10.1073/pnas.1309171110.

Tetrahydrobiopterin Biosynthesis as an Off-Target of Sulfa Drugs

H. Haruki; M. G. Pedersen; K. I. Gorska; F. Pojer; K. Johnsson 

Science. 2013. Vol. 340, num. 6135, p. 987-991. DOI : 10.1126/science.1232972.

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins

G. Lukinavičius; K. Umezawa; N. Olivier; A. Honigmann; G. Yang et al. 

Nature Chemistry. 2013. Vol. 5, num. 2, p. 132-139. DOI : 10.1038/nchem.1546.

Investigating Endocytic Pathways to the Endoplasmic Reticulum and to the Cytosol Using SNAP-Trap

R. Geiger; S. Luisoni; K. Johnsson; U. F. Greber; A. Helenius 

Traffic. 2013. Vol. 14, num. 1, p. 36-46. DOI : 10.1111/tra.12018.

Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation

G. J. Gu; M. Friedman; C. Jost; K. Johnsson; M. Kamali-Moghaddam et al. 

New Biotechnology. 2013. Vol. 30, num. 2, p. 144-152. DOI : 10.1016/j.nbt.2012.05.005.

Selective Chemical Crosslinking Reveals a Cep57-Cep63-Cep152 Centrosomal Complex

G. Lukinavičius; D. Lavogina; M. Orpinell; K. Umezawa; L. Reymond et al. 

Current Biology. 2013. Vol. 23, num. 3, p. 265-270. DOI : 10.1016/j.cub.2012.12.030.

2012

WhiB5, a Transcriptional Regulator That Contributes to Mycobacterium tuberculosis Virulence and Reactivation

S. Casonato; A. Cervantes Sanchez; H. Haruki; M. R. Gonzalez; R. Provvedi et al. 

Infection And Immunity. 2012. Vol. 80, num. 9, p. 3132-3144. DOI : 10.1128/Iai.06328-11.

A Fluorescent Sensor for GABA and Synthetic GABA(B) Receptor Ligands

A. Masharina; L. Reymond; D. Maure; K. Umezawa; K. Johnsson 

Journal Of The American Chemical Society. 2012. Vol. 134, num. 46, p. 19026-19034. DOI : 10.1021/ja306320s.

Exploiting Ligand-Protein Conjugates to Monitor Ligand-Receptor Interactions

H. Haruki; M. R. Gonzalez; K. Johnsson 

Plos One. 2012. Vol. 7, num. 5, p. e37598. DOI : 10.1371/journal.pone.0037598.

Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma

K. Bojkowska; F. Aloisio; M. Cassano; A. Kapopoulou; F. Santoni De Sio et al. 

Hepatology. 2012. Vol. 56, num. 4, p. 1279-1290. DOI : 10.1002/hep.25767.

A Semisynthetic Fluorescent Sensor Protein for Glutamate

M. A. Brun; K-T. Tan; R. Griss; A. Kielkowska; L. Reymond et al. 

Journal Of The American Chemical Society. 2012. Vol. 134, p. 7676-7678. DOI : 10.1021/ja3002277.

A Caged, Localizable Rhodamine Derivative for Superresolution Microscopy

S. Banala; D. Maurel; S. Manley; K. Johnsson 

ACS Chemical Biology. 2012. Vol. 7, num. 2, p. 289-293. DOI : 10.1021/cb2002889.

Directed evolution of the suicide protein O⁶-alkylguanine-DNA alkyltransferase for increased reactivity results in an alkylated protein with exceptional stability

B. Mollwitz; E. Brunk; S. Schmitt; F. Pojer; M. Bannwarth et al. 

Biochemistry. 2012. Vol. 51, num. 5, p. 986-994. DOI : 10.1021/bi2016537.

Benzothiazinones Are Suicide Inhibitors of Mycobacterial Decaprenylphosphoryl-β-

C. Trefzer; H. Škovierová; S. Buroni; A. Bobovská; S. Nenci et al. 

Journal of the American Chemical Society. 2012. Vol. 134, num. 2, p. 912-915. DOI : 10.1021/ja211042r.

2011

Visualizing Biochemical Activities in Living Cells through Chemistry

L. Reymond; G. Lukinavicius; K. Umezawa; D. Maurel; M. A. Brun et al. 

Chimia. 2011. Vol. 65, p. 868-871. DOI : 10.2533/chimia.2011.868.

Switchable fluorophores for protein labeling in living cells

G. Lukinavičius; K. Johnsson 

Current opinion in chemical biology. 2011. Vol. 15, num. 6, p. 768-74. DOI : 10.1016/j.cbpa.2011.10.015.

Development of SNAP-tag fluorogenic probes for wash-free fluorescence imaging

X. Sun; A. Zhang; B. Baker; L. Sun; A. Howard et al. 

Chembiochem : a European journal of chemical biology. 2011. Vol. 12, num. 14, p. 2217-26. DOI : 10.1002/cbic.201100173.

Searching for the Protein Targets of Bioactive Molecules

C. Chidley; H. Haruki; M. G. Pedersen; C. Fellay; S. Moser et al. 

Chimia. 2011. Vol. 65, num. 9, p. 720-724. DOI : 10.2533/chimia.2011.720.

Semisynthesis of fluorescent metabolite sensors on cell surfaces

M. A. Brun; R. Griss; L. Reymond; K-T. Tan; J. Piguet et al. 

Journal of the American Chemical Society. 2011.  p. 110831132027061. DOI : 10.1021/ja206915m.

Measuring In Vivo Protein Half-Life

K. Bojkowska; F. Santoni De Sio; I. Barde; S. Offner; S. Verp et al. 

Chemistry & Biology. 2011. Vol. 18, num. 6, p. 805-815. DOI : 10.1016/j.chembiol.2011.03.014.

Real-Time Measurements of Protein Dynamics Using Fluorescence Activation-Coupled Protein Labeling Method

T. Komatsu; K. Johnsson; H. Okuno; H. Bito; T. Inoue et al. 

Journal of the American Chemical Society. 2011. Vol. 133, num. 17, p. 6745-6751. DOI : 10.1021/ja200225m.

Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag

C. Campos; M. Kamiya; S. Banala; K. Johnsson; M. González-Gaitán 

Developmental Dynamics. 2011. Vol. 240, num. 4, p. 820-827. DOI : 10.1002/dvdy.22574.

A yeast-based screen reveals that sulfasalazine inhibits tetrahydrobiopterin biosynthesis

C. Chidley; H. Haruki; M. G. Pedersen; E. Muller; K. Johnsson 

Nature Chemical Biology. 2011. Vol. 7, num. 6, p. 375-383. DOI : 10.1038/nchembio.557.

2010

Photoactivatable and photoconvertible fluorescent probes for protein labeling

D. Maurel; S. Banala; T. Laroche; K. Johnsson 

ACS chemical biology. 2010. Vol. 5, num. 5, p. 507-16. DOI : 10.1021/cb1000229.

Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5)

M. Geissbuehler; T. Spielmann; A. Formey; I. Märki; M. Leutenegger et al. 

Biophysical journal. 2010. Vol. 98, num. 2, p. 339-49. DOI : 10.1016/j.bpj.2009.10.006.

Phosphopantetheinyl transferase-catalyzed formation of bioactive hydrogels for tissue engineering

K. A. Mosiewicz; K. Johnsson; M. P. Lutolf 

Journal of the American Chemical Society. 2010. Vol. 132, num. 17, p. 5972-4. DOI : 10.1021/ja9098164.

Fura-2FF-based calcium indicator for protein labeling

A. A. Ruggiu; M. Bannwarth; K. Johnsson 

Organic & biomolecular chemistry. 2010. Vol. 8, num. 15, p. 3398-401. DOI : 10.1039/c000158a.

Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore

M. Kamiya; K. Johnsson 

Analytical chemistry. 2010. Vol. 82, num. 15, p. 6472-9. DOI : 10.1021/ac100741t.

Using peptide loop insertion mutagenesis for the evolution of proteins

C. Heinis; K. Johnsson 

Methods in molecular biology (Clifton, N.J.). 2010. Vol. 634, p. 217-232. DOI : 10.1007/978-1-60761-652-8_16.

Benzothiazinones: prodrugs that covalently modify the decaprenylphosphoryl-β-D-ribose 2′-epimerase DprE1 of Mycobacterium tuberculosis

C. Trefzer; M. Rengifo-Gonzalez; M. J. Hinner; P. Schneider; V. Makarov et al. 

Journal of the American Chemical Society. 2010. Vol. 132, num. 39, p. 13663-5. DOI : 10.1021/ja106357w.

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

C. Dellagiacoma; G. Lukinavicius; N. Bocchio; S. Banala; S. Geissbühler et al. 

Chembiochem -Weinheim-. 2010. Vol. 11, p. 1361-1363. DOI : 10.1002/cbic.201000189.

2009

EMBL Chemical Biology Meeting 2008: New Views and Interviews

M. J. Hinner 

Acs Chemical Biology. 2009. Vol. 4, p. 13-17. DOI : 10.1021/cb800315z.

Selective cross-linking of interacting proteins using self-labeling tags

A. Gautier; E. Nakata; G. Lukinavicius; K-T. Tan; K. Johnsson 

Journal of the American Chemical Society. 2009. Vol. 131, num. 49, p. 17954-62. DOI : 10.1021/ja907818q.

Indo-1 derivatives for local calcium sensing

M. Bannwarth; I. Reis Correa Jr.; M. Sztretye; S. Pouvreau; C. Fellay et al. 

ACS Chemical Biology. 2009. Vol. 4, num. 3, p. 179-190. DOI : 10.1021/cb800258g.

Visualizing biochemical activities in living cells

K. Johnsson 

Nature Chemical Biology. 2009. Vol. 5, p. 63-65. DOI : 10.1038/nchembio0209-63.

Semisynthetic fluorescent sensor proteins based on self-labeling protein tags

M. A. Brun; K-T. Tan; E. Nakata; M. J. Hinner; K. Johnsson 

Journal of the American Chemical Society. 2009. Vol. 131, num. 16, p. 5873-5884. DOI : 10.1021/ja900149e.

2008

Regulation of glutamate metabolism by protein kinases in mycobacteria

H. M. O’Hare; R. Durán; C. Cerveñansky; M. Bellinzoni; A. M. Wehenkel et al. 

Molecular Microgiology. 2008. Vol. 70, p. 1408-1423. DOI : 10.1111/j.1365-2958.2008.06489.x.

Photobleaching-free infrared near-field microscopy localizes molecules in neurons

J. Generosi; G. Margaritondo; M. Kropf; H. Hirling; S. Catsicas et al. 

Journal of Applied Physics. 2008. Vol. 104, p. 106102. DOI : 10.1063/1.3003567.

Subunit-specific surface mobility of differentially labeled AMPA receptor subunits

M. Kropf; G. Rey; L. Glauser; K. Kulangara; K. Johnsson et al. 

European Journal of Cell Biology. 2008. Vol. 87, p. 763-778. DOI : 10.1016/j.ejcb.2008.02.014.

A designed protein for the specific and covalent hetero-conjugation of biomolecules

C. Chidley; K. Mosiewicz; K. Johnsson 

Bioconjugate Chemistry. 2008. Vol. 19, p. 1753-1756. DOI : 10.1021/bc800268j.

AMPA receptor imaging by infrared scanning near-field optical microscopy

J. Generosi; M. Kropf; G. Margaritondo; H. Hirling; S. Catsicas et al. 

Physica Status Solidi (C). 2008. Vol. 5, num. 8, p. 2641-2644. DOI : 10.1002/pssc.200779116.

An engineered protein tag for multi-protein labeling in living cells

A. Gautier; A. Juillerat; C. Heinis; I. Reis Correa Jr.; M. Kindermann et al. 

Chemistry & Biology. 2008. Vol. 15, p. 128-63. DOI : 10.1016/j.chembiol.2008.01.007.

A split-protein sensor for studying protein-protein interactions in mycobacteria

H. O’Hare; A. Juillerat; P. Dianiskova; K. Johnsson 

Journal of Microbiological Methods. 2008. Vol. 73, p. 79-84. DOI : 10.1016/j.mimet.2008.02.008.

Caged substrates for protein labeling and immobilization

S. Banala; A. Arnold; K. Johnsson 

ChemBioChem. 2008. Vol. 9, p. 38-41. DOI : 10.1002/cbic.200700472.

2007

Chemical probes shed light on fusion proteins

H. O’Hare; K. Johnsson; A. Gautier 

Current Opinion in Structural Biology. 2007. Vol. 17, num. 4, p. 488-494. DOI : 10.1016/j.sbi.2007.07.005.

A Covalent Chemical Genotype-Phenotype Linkage for in vitro Protein Evolution

V. Stein; I. Sielaff; K. Johnsson; F. Hollfelder 

ChemBioChem. 2007. Vol. 8, p. 2191-2194. DOI : 10.1002/cbic.200700459.

Chemical tools for biomolecular imaging

N. Johnsson; K. Johnsson 

ACS Chemical Biology. 2007. Vol. 2, p. 31-38. DOI : 10.1021/cb6003977.

Inducing and sensing protein-protein interactions in living cells via selective crosslinking

G. Lemercier; S. Gendreizig; M. Kindermann; K. Johnsson 

Angewandte Chemie. 2007. Vol. 46, p. 4281-4285. DOI : 10.1002/anie.200700408.

2006

Evolving the substrate specificity of O6-alkylguanine-DNA alkyltransferase through loop insertion for applications in molecular imaging

C. Heinis; S. Schmitt; M. Kindermann; G. Godin; K. Johnsson 

ACS Chemical Biology. 2006. Vol. 1, p. 575-584. DOI : 10.1021/cb6003146.

Chimeric streptavidins with reduced valencies

G. Lemercier; K. Johnsson 

Nature Methods. 2006. Vol. 3, p. 247-8. DOI : 10.1038/nmeth0406-247.

Directed evolution of O6-alkylguanine-DNA alkyltransferase for applications in protein labeling

T. Gronemeyer; C. Chidley; A. Juillerat; C. Heinis; K. Johnsson 

Protein Engineering, Design and Selection. 2006. Vol. 19, num. 7, p. 309-318. DOI : 10.1093/protein/gzl014.

Protein function microarrays based on self-immobilizing and self-labeling fusion proteins

I. Sielaff; A. Arnold; G. Godin; S. Tugulu; H-A. Klok et al. 

ChemBioChem. 2006. Vol. 7, num. 1, p. 194-202. DOI : 10.1002/cbic.200500278.

Posttranslational covalent labeling reveals heterogeneous mobility of individual G protein-coupled receptors in living cells

M. Prummer; B. H. Meyer; R. Franzini; J-M. Segura; N. George et al. 

ChemBioChem. 2006. Vol. 7, num. 6, p. 908-911. DOI : 10.1002/cbic.200500477.

Covalent labeling of cell-surface proteins for in-vivo FRET studies

B. H. Meyer; K. L. Martinez; J-M. Segura; P. Pascoal; R. Hovius et al. 

FEBS Letters. 2006. Vol. 580, num. 6, p. 1654-1658. DOI : 10.1016/j.febslet.2006.02.007.

FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells

H. Meyer Bruno; J-M. Segura; L. Martinez Karen; R. Hovius; N. George et al. 

Proceedings of the National Academy of Sciences of the United States of America. 2006. Vol. 103, num. 7, p. 2138-2143. DOI : 10.1073/pnas.0507686103.

2005

Adding value to fusion proteins through covalent labeling

T. Gronemeyer; G. Godin; K. Johnsson 

Current Opinion in Biotechnology. 2005. Vol. 16, num. 4, p. 453-458. DOI : 10.1016/j.copbio.2005.06.001.

Engineering substrate specificity of O6-alkylguanine-DNA alkyltransferase for specific protein labeling in living cells

A. Juillerat; C. Heinis; I. Sielaff; J. Barnikow; H. Jaccard et al. 

ChemBioChem. 2005. Vol. 6, num. 7, p. 1263-1269. DOI : 10.1002/cbic.200400431.

Fast directed evolution of non-immunoglobulin proteins by somatic hypermutation in immune cells

C. Heinis; K. Johnsson 

ChemBioChem. 2005. Vol. 6, num. 5, p. 804-806. DOI : 10.1002/cbic.200500011.

The Laboratory in a Droplet

H. M. O’Hare; K. Johnsson 

Chemistry & Biology. 2005. Vol. 12, num. 12, p. 1255-1257.

Multicolor Imaging of Cell Surface Proteins

L. Vivero-Pol; N. George; H. Krumm; K. Johnsson; N. Johnsson 

Journal of the American Chemical Society. 2005. Vol. 127, num. 37, p. 12770-12771. DOI : 10.1021/ja0533850.

Protein chemistry on the surface of living cells

N. Johnsson; N. George; K. Johnsson 

ChemBioChem. 2005. Vol. 6, num. 1, p. 47-52. DOI : 10.1002/cbic.200400290.

Protein-Functionalized Polymer Brushes

S. Tugulu; A. Arnold; I. Sielaff; K. Johnsson; H-A. Klok et al. 

Biomacromolecules. 2005. Vol. 6, num. 3, p. 1602-1607. DOI : 10.1021/bm050016n.

2004

A combined RSA and FE study of the implanted proximal tibia: correlation of the post-operative mechanical environment with implant migration

A. Perillo-Marcone; L. Ryd; K. Johnsson; M. Taylor 

Journal of biomechanics. 2004. Vol. 37, num. 8, p. 1205-13.

Labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase with small molecules in vivo and in vitro

A. Keppler; M. Kindermann; S. Gendreizig; H. Pick; H. Vogel et al. 

Methods. 2004. Vol. 32, num. 4, p. 437-444. DOI : 10.1016/j.ymeth.2003.10.007.

Labeling of fusion proteins with synthetic fluorophores in live cells

A. Keppler; H. Pick; C. Arrivoli; H. Vogel; K. Johnsson 

Proceedings of the National Academy of Sciences of the United States of America. 2004. Vol. 101, num. 27, p. 9955-9959. DOI : 10.1073/pnas.0401923101.

Specific Labeling of Cell Surface Proteins with Chemically Diverse Compounds

N. George; H. Pick; H. Vogel; N. Johnsson; K. Johnsson 

Journal of the American Chemical Society. 2004. Vol. 126, num. 29, p. 8896-8897. DOI : 10.1021/ja048396s.

Synthesis and characterization of bifunctional probes for the specific labeling of fusion proteins

M. Kindermann; I. Sielaff; K. Johnsson 

Bioorganic & Medicinal Chemistry Letters. 2004. Vol. 14, num. 11, p. 2725-2728. DOI : 10.1016/j.bmcl.2004.03.078.

Transforming a (beta/alpha)8-Barrel Enzyme into a Split-Protein Sensor through Directed Evolution

P. Tafelmeyer; N. Johnsson; K. Johnsson 

Chemistry & Biology. 2004. Vol. 11, num. 5, p. 681-689.

2003

Covalent and selective immobilization of fusion proteins

M. Kindermann; N. George; N. Johnsson; K. Johnsson 

Journal of the American Chemical Society. 2003. Vol. 125, num. 26, p. 7810-7811. DOI : 10.1021/ja034145s.

Covalent labeling of fusion proteins with chemical probes in living cells

S. Gendreizig; A. Keppler; A. Juillerat; T. Gronemeyer; H. Pick et al. 

Chimia. 2003. Vol. 57, num. 4, p. 181-183.

Directed Evolution of O6-Alkylguanine-DNA Alkyltransferase for Efficient Labeling of Fusion Proteins with Small Molecules In Vivo

A. Juillerat; T. Gronemeyer; A. Keppler; S. Gendreizig; H. Pick et al. 

Chemistry & Biology. 2003. Vol. 10, num. 4, p. 313-317. DOI : 10.1016/S1074-5521(03)00068-1.

DNA repair protein O6-methylguanine-DNA methyltransferase in testis and testicular tumors as determined by a novel nonradioactive assay

G. Nagel; W. Brenner; K. Johnsson; B. Kaina 

Analytical Biochemistry. 2003. Vol. 321, num. 1, p. 38-43. DOI : 10.1016/S0003-2697(03)00432-9.

A fusion of disciplines: Chemical approaches to exploit fusion proteins for functional genomics

N. Johnsson; K. Johnsson 

ChemBioChem. 2003. Vol. 4, num. 9, p. 803-810.

A general method for the covalent labeling of fusion proteins with small molecules in vivo

A. Keppler; S. Gendreizig; T. Gronemeyer; H. Pick; H. Vogel et al. 

Nature Biotechnology. 2003. Vol. 21, num. 1, p. 86-89. DOI : 10.1038/nbt765.

Induced Protein Dimerization in Vivo through Covalent Labeling

S. Gendreizig; M. Kindermann; K. Johnsson 

Journal of the American Chemical Society. 2003. Vol. 125, num. 49, p. 14970-14971. DOI : 10.1021/ja037883p.

2002

Examining reactivity and specificity of cytochrome c peroxidase by using combinatorial mutagenesis

M. Wilming; A. Iffland; P. Tafelmeyer; C. Arrivoli; C. Saudan et al. 

ChemBioChem. 2002. Vol. 3, num. 11, p. 1097-1104. DOI : 10.1002/1439-7633(20021104)3:11<1097::AID-CBIC1097>3.0.CO;2-4.

37th ESF/EUCHEM conference on stereochemistry

K. Severin; K. Johnsson 

Chimia. 2002. Vol. 56, num. 7-8, p. 432-438.

Towards the generation of artificial O6-alkylguanine-DNA alkyltransferases: In vitro selection of antibodies with reactive cysteine residues

R. Damoiseaux; P. G. Schultz; K. Johnsson 

ChemBioChem. 2002. Vol. 3, num. 6, p. 573-575. DOI : 10.1002/1439-7633(20020603)3:6<573::AID-CBIC573>3.0.CO;2-F.

2001

Changing the substrate specificity of cytochrome c peroxidase using directed evolution

A. Iffland; S. Gendreizig; P. Tafelmeyer; K. Johnsson 

Biochemical and Biophysical Research Communications. 2001. Vol. 286, num. 1, p. 126-132. DOI : 10.1006/bbrc.2001.5366.

Evolutionary approaches to study cytochrome c peroxidase

A. Iffland; P. Tafelmeyer; S. Gendreizig; K. Johnsson 

Chimia. 2001. Vol. 55, num. 4, p. 291-294.

Inter- and intramolecular domain interactions of the catalase-peroxidase KatG from M. tuberculosis

M. Wilming; K. Johnsson 

FEBS Letters. 2001. Vol. 509, num. 2, p. 272-276. DOI : 10.1016/S0014-5793(01)03179-9.

Synthesis and applications of chemical probes for human O6-alkylguanine-DNA alkyltransferase

R. Damoiseaux; A. Keppler; K. Johnsson 

ChemBioChem. 2001. Vol. 2, num. 4, p. 285-287. DOI : 10.1002/1439-7633(20010401)2:4<285::AID-CBIC285>3.0.CO;2-N.

2000

Directed molecular evolution of cytochrome c peroxidase

A. Iffland; P. Tafelmeyer; C. Saudan; K. Johnsson 

Biochemistry. 2000. Vol. 39, num. 35, p. 10790-10798. DOI : 10.1021/bi001121e.

1999

Use of site-directed mutagenesis to probe the structure, function and isoniazid activation of the catalase/peroxidase, KatG, from Mycobacterium tuberculosis

B. Saint-Joanis; H. Souchon; M. Wilming; K. Johnsson; P. M. Alzari et al. 

The Biochemical journal. 1999. Vol. 338, num. 3, p. 753-60. DOI : 10.1042/0264-6021:3380753.

Phage display of combinatorial peptide and protein libraries and their applications in biology and chemistry

K. Johnsson; L. Ge 

Current Topics in Microbiology and Immunology. 1999. Vol. 243, num. Combinatorial Chemistry in Biology, p. 87-105.

Spontaneous formation of the bioactive form of the tuberculosis drug isoniazid

M. Wilming; K. Johnsson 

Angewandte Chemie, International Edition. 1999. Vol. 38, num. 17, p. 2588-2590. DOI : 10.1002/(SICI)1521-3773(19990903)38:17<2588::AID-ANIE2588>3.0.CO;2-8.

Trends in organic chemistry in 1998. Part 1

N. Goldberg; H-U. Siehl; T. Mueller; C. Strohmann; B. Breit et al. 

Nachrichten aus Chemie, Technik und Laboratorium. 1999. Vol. 47, num. 2, p. 153-175.

1997

Overexpression, purification, and characterization of the catalase-peroxidase KatG from Mycobacterium tuberculosis

K. Johnsson; W. A. Froland; P. G. Schultz 

Journal of Biological Chemistry. 1997. Vol. 272, num. 5, p. 2834-2840.

1996

Organization of ‘nanocrystal molecules’ using DNA

A. P. Alivisatos; K. P. Johnsson; X. Peng; T. E. Wilson; C. J. Loweth et al. 

Nature. 1996. Vol. 382, num. 6592, p. 609-611. DOI : 10.1038/382609a0.

1995

Studies on the Mechanism of Action of Isoniazid and Ethionamide in the Chemotherapy of Tuberculosis

K. Johnsson; D. S. King; P. G. Schultz 

Journal of the American Chemical Society. 1995. Vol. 117, num. 17, p. 5009-10. DOI : 10.1021/ja00122a038.

1994

Mechanistic Studies of the Oxidation of Isoniazid by the Catalase Peroxidase from Mycobacterium tuberculosis

K. Johnsson; P. G. Schultz 

Journal of the American Chemical Society. 1994. Vol. 116, num. 16, p. 7425-6. DOI : 10.1021/ja00095a063.

1993

Synthesis, structure and activity of artificial, rationally designed catalytic polypeptides

K. Johnsson; R. K. Allemann; H. Widmer; S. A. Benner 

Nature. 1993. Vol. 365, num. 6446, p. 530-2.

Triple helix binding of oligodeoxyribonucleotides containing 8-oxo-2′-deoxyadenosine

E. C. Davison; K. Johnsson 

Nucleosides & Nucleotides. 1993. Vol. 12, num. 2, p. 237-43.

1989

Annealing of divacancy-related infrared absorption bands in boron-doped silicon

B. G. Svensson; K. Johnsson; D. X. Xu; J. H. Svensson; J. L. Lindstroem 

Radiation Effects and Defects in Solids. 1989. Vol. 111-112, num. 1-2, p. 439-47.

1988

A discontinuous epitope on p36, the major substrate of src tyrosine-protein-kinase, brings the phosphorylation site into the neighborhood of a consensus sequence for calcium/lipid-binding proteins

N. Johnsson; K. Johnsson; K. Weber 

FEBS Letters. 1988. Vol. 236, num. 1, p. 201-4. DOI : 10.1016/0014-5793(88)80314-4.

Conference Papers

2015

Modulating protein-protein interaction with a molecular tether

H. Farrants; O. Hantschel; K. Johnsson 

2015. 29th Annual Symposium of the Protein-Society. p. 213-214.

2013

Certain cancer and RASopathy associated mutations affect Ras nanoclustering

D. Abankwa; C. Guzman; M. Solman; E. Siljamaki; A. Ligabue et al. 

2013. 38th Congress of the Federation-of-European-Biochemical-Societies (FEBS). p. 406-407.

2012

Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5)

M. Geissbühler; T. Spielmann; A. Formey de Saint Louvent; I. Märki; M. Leutenegger et al. 

2012. Conference of international society on oxygen transport to tissue, Monte Verità, Ascona, Switzerland, July 18-23, 2010. p. 263-268. DOI : 10.1007/978-1-4614-1566-4_39.

2005

Protein-functionalized polymer brushes

H-A. Klok; S. Tugulu; A. Arnold; I. Sielaff; K. Johnsson 

2005.  p. POLY-640.

2003

Protein chemistry in living cells

K. Johnsson 

2003.  p. ORGN-695.

1990

Designed enzymes: new peptides that fold in aqueous solution and catalyze reactions

K. Johnsson; R. K. Allemann; S. A. Benner 

1990. Royal Society of Chemistry Joint Perkin Division – Bioorganic Group Meeting, University of Newcastle-upon-Tyne, United Kingdom, July 17-20, 1989. p. 166-87.

Reviews

2019

Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy

L. Wang; M. S. Frei; A. Salim; K. Johnsson 

Journal Of The American Chemical Society. 2019-02-20. Vol. 141, num. 7, p. 2770-2781. DOI : 10.1021/jacs.8b11134.

2010

How to obtain labeled proteins and what to do with them

M. J. Hinner; K. Johnsson 

Current Opinion In Biotechnology. 2010. Vol. 21, p. 766-776. DOI : 10.1016/j.copbio.2010.09.011.

Books

2002

Directed Molecular Evolution of Proteins or How to Improve Enzymes for Biocatalysis

S. Brakmann; K. Johnsson 

Weinheim: Wiley, 2002.

Theses

2019

Allosteric Protein Switches based on Antibody Mimetics

H. R. O. Farrants / K. Johnsson (Dir.)  

Lausanne, EPFL, 2019. 

2018

Cofactor sensing proteins for metabolic assays at point-of-care

Q. Yu / K. Johnsson (Dir.)  

Lausanne, EPFL, 2018. 

Semisynthetic Biosensors for the Quantification of Nicotinamide Adenine Dinucleotides in Live Cells

O. V. Sallin / V. Hatzimanikatis; K. Johnsson (Dir.)  

Lausanne, EPFL, 2018. 

Towards Elucidating the Mechanism of Action of Biguanides

N. P. Goeldel / K. Johnsson; V. Hatzimanikatis (Dir.)  

Lausanne, EPFL, 2018. 

Discovery of novel competitive dihydropteridine reductase inhibitors via high throughput screening and their effect on metabolic pterin balance of fibroblast.

Y. M. Pierson / K. Johnsson; V. Hatzimanikatis (Dir.)  

Lausanne, EPFL, 2018. 

2017

Fluorescent sensor proteins to assess the cellular uptake of therapeutic peptides and small molecules

S. Scarabelli / K. Johnsson (Dir.)  

Lausanne, EPFL, 2017. 

2016

Fluorescent Probes for Plasma Membrane Proteins Based on Nile Red

E. Prifti / K. Johnsson (Dir.)  

Lausanne, EPFL, 2016. 

2014

Inhibition of Sepiapterin Reductase through Formation of a Ternary Complex with Sulfasalazine or other Sulfa Drugs and NADP+

M. G. Pedersen / K. Johnsson (Dir.)  

Lausanne, EPFL, 2014. 

Sensing Acetylcholine and Anticholinesterases and Generalization of Semisynthetic Biosensors

A. Schena / K. Johnsson (Dir.)  

Lausanne, EPFL, 2014. 

2013

Bioluminescent sensor proteins for point-of-care therapeutic drug monitoring

R. Griss / K. Johnsson (Dir.)  

Lausanne, EPFL, 2013. 

Detection of Drug-Protein Interactions with an Improved Yeast Three-Hybrid System

C. Fellay / K. Johnsson (Dir.)  

Lausanne, EPFL, 2013. 

2012

SNAP-tag based FRET Indicator for GABA and Synthetic GABAB Receptor Ligands

A. Masharina / K. Johnsson (Dir.)  

Lausanne, EPFL, 2012. 

DprE1 as a Drug Target from Mycobacterium Tuberculosis

C. Trefzer / K. Johnsson (Dir.)  

Lausanne, EPFL, 2012. 

Structural Studies and Protein Engineering of Human O6-Alkylguanine-DNA Alkyltransferase

B. Mollwitz / K. Johnsson (Dir.)  

Lausanne, EPFL, 2012. 

2011

In Vivo Studies on the Control of Gene Expression and Protein Degradation

K. Bojkowska / K. Johnsson; D. Trono (Dir.)  

Lausanne, EPFL, 2011. 

Semisynthetic Fluorescent Sensor Proteins Based on Self-Labeling Protein Tags

M. A. Brun / K. Johnsson (Dir.)  

Lausanne, EPFL, 2011. 

2010

A Novel Yeast-Based Platform for Small Molecule Target Deconvolution Reveals Previously Unknown Binding Partners for the Approved Drugs Erlotinib, Atorvastatin, and Sulfasalazine

C. Chidley / K. Johnsson (Dir.)  

Lausanne, EPFL, 2010. 

Photoactivatable Probes for Protein Labeling

S. Banala / K. Johnsson (Dir.)  

Lausanne, EPFL, 2010. 

The role of sulfated metabolites in the wound response of siphonous green algae

M. A. Welling / K. Johnsson; G. Pohnert (Dir.)  

Lausanne, EPFL, 2010. 

2008

Synthesis of calcium sensors for labelling fusion proteins-synthesis of modified prolines using aza naked sugar methodology

A. Ruggiu / K. Johnsson (Dir.)  

Lausanne, EPFL, 2008. 

2007

O6-alkylguanine-DNA alkyltransferase as a new tool for functional proteomics

A. Arnold / K. Johnsson (Dir.)  

Lausanne, EPFL, 2007. 

2006

A new method for protein labeling with small molecules based on acyl carrier protein

N. George / K. Johnsson (Dir.)  

Lausanne, EPFL, 2006. 

Chemical approaches to microarrays and multifunctional protein labeling

I. C. Sielaff / K. Johnsson (Dir.)  

Lausanne, EPFL, 2006. 

Book Chapters

2017

Rational Design and Applications of Semisynthetic Modular Biosensors: SNIFITs and LUCIDs

H. Farrants; J. Hiblot; R. Griss; K. Johnsson 

Synthetic Protein Switches; New York, NY: Springer New York, 2017. p. 101-117.

Highly Modular Bioluminescent Sensors for Small Molecules and Proteins

Q. Yu; R. Griss; A. Schena; K. Johnsson 

Enzymes As Sensors; San Diego: Elsevier Academic Press Inc, 2017. p. 365-382.

Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor

G. Hassaïne; C. Deluz; L. Grasso; R. Wyss; R. Hovius et al. 

Membrane Protein Structure and Function Characterization; New York, NY: Springer New York, 2017. p. 139-168.

2015

Fluorescent Labeling of SNAP-Tagged Proteins in Cells

G. Lukinavičius; L. Reymond; K. Johnsson 

Site-Specific Protein Labeling; New York: Springer, 2015. p. 107-118.

2004

Directed molecular evolution of proteins

P. Tafelmeyer; K. Johnsson 

Highlights in Bioorganic Chemistry; Weinheim: Wiley, 2004. p. 341-343.

Labeling of fusion proteins with small molecular in vivo

S. Gendreizig; A. Keppler; A. Juillerat; T. Gronemeyer; K. Johnsson 

Highlights in Bioorganic Chemistry; Weinheim: Wiley, 2004. p. 344-351.

Patents

2017

Sepiapterin reductase inhibitors

M. J. Tebbe; H. V. Atton; C. Avery; S. M. Bromidge; M. Kerry et al. 

JP2018529739; EP3356345; US9963462; US2017096435; CA3038280; WO2017059191.

2017.

2016

Means and methods for bioluminescence resonance energy transfer (bret) analysis in a biological sample

K. P. Johnsson; A. Schena; R. Griss 

US9952208; EP3022316; US2016146794; EP3022316; CN105555964; AU2013394649; CA2918426; WO2015007317.

2016.

Sensors, methods and kits for detecting nicotinamide adenine dinucleotides

O. Sallin; L. Reymond; K. P. Johnsson 

US10221439; US2018087083; JP2018506285; EP3259365; WO2016131833.

2016.

2015

Sensor molecules and uses thereof

K. P. Johnsson; A. Schena; R. Griss 

WO2015067302.

2015.

2013

Labelling of Fusion Proteins with Synthetic Probes

A. Gautier; K. Johnsson; M. Kindermann; A. Juillerat; F. Beaufils 

US2013130291.

2013.

Cell permeable, fluorescent dye

K. Umezawa; L. Grazvydas; K. Johnsson 

US9701841; EP2748173; US2016230011; US9346957; US2014349282; EP2748173; WO2013029650.

2013.

2011

Compositions and use of sulfasalazine

K. Johnsson; C. Chidley 

WO2011138142.

2011.

2008

Labelling of fusion proteins with synthetic probes

A. Gautier; K. Johnsson; M. Kindermann; A. Juillerat; F. Beaufils 

US8623627; EP2049499; US2012237961; US8227602; US2011207157; JP2009544660; CN101516857; EP2049499; WO2008012296; EP1882688.

2008.

Method of crosslinking two objects of interest

K. Johnsson; C. Chidley; K. Mosiewicz 

US2010075394; WO2008122604; EP1978093.

2008.

2006

Mutants of o6-alkylguanine-dna alkyltransferase

J. Barnikov; C. Chidley; T. Gronemeyer; C. Heinis; H. Jaccard et al. 

US2011165593; US7888090; JP2007525988; US2007207532; EP1720981; WO2005085431; WO2005085431; WO2005085431.

2006.

Specific substrates for O6- alkylguanine-DNA alkyltransferase

H. Jaccard; K. Johnsson; M. Kindermann; I. Sielaff 

EP1730298; US8163479; US2007243568; JP2007526282; EP1730298; WO2005085470; WO2005085470.

2006.

2005

Method for identification of suitable fragmentation sites in a reporter protein

K. Johnsson; P. Tafelmeyer 

DE602004030270; AT489480; EP1670940; US2009305286; US2007178460; EP1670940; CA2542182; WO2005038050.

2005.

Method for identification of fragmentation sites in a reporter protein in the development of split protein sensors

K. Johnsson; P. Tafelmeyer 

DE602004030270; AT489480; EP1670940; US2009305286; US2007178460; EP1670940; WO2005038050; CA2542182.

2005.

Mutants of human O6-alkylguanine-DNA alkyltransferase with improved properties

J. Barnikov; C. Chidley; T. Gronemeyer; C. Heinis; H. Jaccard et al. 

US2011165593; US7888090; JP2007525988; US2007207532; EP1720981; WO2005085431; WO2005085431; WO2005085431.

2005.

2004

Methods for protein labeling based on acyl carrier protein

K. Johnsson; N. George 

US8476031; CN1826527; US2010173384; US7666612; NZ543653; ZA200509454; JP2007509608; US2007082336; CN1826527; EP1627226; KR20060015612; AU2004242249; CA2526579; WO2004104588.

2004.

Substrates for o<6>-alkylguanine-dna alkyltransferase

M. Kindermann; K. Johnsson; C. Bieri 

JP4976651; US7799524; NZ538986; ZA200502210; US2006024775; JP2006501289; CN1720332; EP1546371; KR20050062588; AU2003271669; CA2501063; WO2004031405.

2004.

Methods using o<6>-alkylguanine-dna alkyltransferases

K. Johnsson; S. Gendreizig; A. Keppler; S. Kiddle 

EP2211177; US8367361; CN1975422; US2011201514; US7939284; DE60237792; AT482395; EP2037271; EP2211177; EP2037271; JP4226053; DE60230689; AT419378; EP1696234; JP4195815; JP2008154587; HK1068406; AU2002251257; DE60212642; CN1975422; CN1295510; DK1410023; EP1696234; DE60212642; AT331220; NZ540410; EP1410023; NZ537939; NZ528594; JP2004532028; CN1527941; ZA200307442; US2004115130; KR20040039194; EP1410023; IL157880; WO02083937; CA2443570; WO02083937.

2004.

Protein labelling with o6-alkylguanine-dna alkyltransferase

A. Juillerat; A. Keppler; K. Johnsson; T. Gronemeier; S. Gendreizig et al. 

US2006292651; ZA200502211; JP2006501286; CN1717496; EP1546370; KR20050049513; AU2003267423; CA2501061; WO2004031404.

2004.

Guanine derivatives as substrates for O6-alkylguanine-DNA alkyltransferase and their use in labeling the enzyme and fusion proteins

M. Kindermann; K. Johnsson; C. Bieri 

JP4976651; US7799524; NZ538986; ZA200502210; US2006024775; JP2006501289; CN1720332; EP1546371; KR20050062588; AU2003271669; WO2004031405; CA2501063.

2004.

Labeling of fusion proteins by enzymic incorporation of a coenzyme A derivative into an acyl carrier protein moiety

K. Johnsson; N. George 

US8476031; CN1826527; US2010173384; US7666612; NZ543653; ZA200509454; JP2007509608; US2007082336; CN1826527; EP1627226; KR20060015612; WO2004104588; AU2004242249; CA2526579.

2004.

O6-alkylguanine-DNA alkyltransferases for efficient labeling of fusion proteins

A. Juillerat; A. Keppler; K. Johnsson; T. Gronemeier; S. Gendreizig et al. 

US2006292651; ZA200502211; JP2006501286; CN1717496; EP1546370; KR20050049513; AU2003267423; WO2004031404; CA2501061.

2004.

2002

Methods using O6-alkylguanine-DNA alkyltransferases

S. J. Kiddle; K. Johnsson; S. Gendreizig; A. Keppler 

EP2211177; US8367361; CN1975422; US2011201514; US7939284; DE60237792; AT482395; EP2037271; EP2211177; EP2037271; JP4226053; DE60230689; AT419378; EP1696234; JP4195815; JP2008154587; HK1068406; AU2002251257; DE60212642; CN1975422; CN1295510; DK1410023; EP1696234; DE60212642; AT331220; NZ540410; EP1410023; NZ537939; NZ528594; JP2004532028; CN1527941; ZA200307442; US2004115130; KR20040039194; EP1410023; IL157880; WO02083937; WO02083937; CA2443570.

2002.

2000

Selective immobilization of active 6-O-Alkylguanine DNA alkyltransferase for its determination by transferring a coupling group to the enzyme from a 6-O-Alkylguanine derivative

K. Johnsson 

DE19903895.

2000.