This aim of this project is to develop an high-throughput analytical method to identify glycans from biological samples. The challenge of doing so arises from the isomeric complexity of glycans, which is difficult to resolve by any single analytical tool. Our approach combines ultrahigh resolution ion mobility based on structures for lossless ion manipulations (SLIM) with cryogenic ion spectroscopy and mass spectrometry to perform glycan analysis at high throughput.
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See some of our recent papers for details:
Combining Liquid Chromatography and Cryogenic IR Spectroscopy in Real Time for the Analysis of Oligosaccharides
Analytical Chemistry. 2024-01-11. Vol. 96, num. 4, p. 1462-1467. DOI : 10.1021/acs.analchem.3c03578.Using Hadamard Transform Multiplexed IR Spectroscopy Together with a Segmented Ion Trap for the Identification of Mobility-Selected Isomers
Analytical Chemistry. 2023-06-12. DOI : 10.1021/acs.analchem.3c01340.New Approach for the Identification of Isobaric and Isomeric Metabolites
Analytical Chemistry. 2023-04-29. Vol. 95, num. 18, p. 7118-7126. DOI : 10.1021/acs.analchem.2c04962.Identification of human milk oligosaccharide positional isomers by combining IMS-CID-IMS and cryogenic IR spectroscopy
Analyst. 2023-04-21. Vol. 148, num. 10, p. 2277-2282. DOI : 10.1039/d3an00407d.Multistage Ion Mobility Spectrometry Combined with Infrared Spectroscopy for Glycan Analysis
Journal Of The American Society For Mass Spectrometry. 2023-03-07. DOI : 10.1021/jasms.2c00361.Device, system, and method for ion fragmentation by use of an ion mobility device and messenger tagging
US2023317442.
2023.Identification of Mobility-Resolved N-Glycan Isomers
Analytical Chemistry. 2022-07-07. Vol. 94, num. 28, p. 10101-10108. DOI : 10.1021/acs.analchem.2c01181.A New Strategy Coupling Ion-Mobility-Selective CID and Cryogenic IR Spectroscopy to Identify Glycan Anomers
Journal of the American Society for Mass Spectrometry. 2022-04-19. Vol. 33, num. 5, p. 859-864. DOI : 10.1021/jasms.2c00043.A New Approach for Identifying Positional Isomers of Glycans Cleaved from Monoclonal Antibodies
Chimia. 2022-04-01. Vol. 76, num. 4, p. 363-363. DOI : 10.2533/chimia.2022.363.High-Throughput Multiplexed Infrared Spectroscopy of Ion Mobility-Separated Species Using Hadamard Transform
Analytical Chemistry. 2022-02-03. Vol. 94, num. 6, p. 2912–2917. DOI : 10.1021/acs.analchem.1c04843.Identification of N-glycan positional isomers by combining IMS and vibrational fingerprinting of structurally determinant CID fragments
Analyst. 2022-01-21. DOI : 10.1039/d1an01861b.A method and system to build an ir fingerprint database for the structural identification of biomolecules
WO2022259096; WO2022259096.
2022.Identifying Mixtures of Isomeric Human Milk Oligosaccharides by the Decomposition of IR Spectral Fingerprints
Analytical Chemistry. 2021-10-27. Vol. 93, num. 44, p. 14730-14736. DOI : 10.1021/acs.analchem.1c03190.Toward High-Throughput Cryogenic IR Fingerprinting of Mobility-Separated Glycan Isomers
ACS Measurement Science Au. 2021-09-06. Vol. 1, num. 3, p. 157–164. DOI : 10.1021/acsmeasuresciau.1c00018.A new approach for identifying positional isomers of glycans cleaved from monoclonal antibodies
Analyst. 2021-06-30. Vol. 146, p. 4789-4795. DOI : 10.1039/d1an00780g.Using SLIM-Based IMS-IMS Together with Cryogenic Infrared Spectroscopy for Glycan Analysis
Analytical Chemistry. 2020-07-07. Vol. 92, num. 13, p. 9079-9085. DOI : 10.1021/acs.analchem.0c01265.How General Is Anomeric Retention during Collision-Induced Dissociation of Glycans?
Journal of the American Chemical Society. 2020-03-16. Vol. 142, num. 13, p. 5948-5951. DOI : 10.1021/jacs.0c00264.Combining ultra-high resolution ion mobility with cryogenic ion vibrational spectroscopy for the analysis of glycans
2019-03-31. National Meeting of the American-Chemical-Society (ACS), Orlando, FL, Mar 31-Apr 04, 2019.High-throughput cryogenic spectroscopy for glycan analysis
US10522337; US2019180997.
2019.Separation and Identification of Glycan Anomers Using Ultrahigh-Resolution Ion Mobility Spectrometry and Cryogenic Ion Spectroscopy
Journal of the American Society for Mass Spectrometry. 2019. Vol. 30, num. 11, p. 2204-2211. DOI : 10.1007/s13361-019-02333-0.Combining ultra-high resolution ion-mobility spectrometry with cryogenic IR spectroscopy for the study of biomolecular ions
Faraday Discussions. 2019. Vol. 217, p. 114-125. DOI : 10.1039/C8FD00180D.Combining Ultrahigh-Resolution Ion-Mobility Spectrometry with Cryogenic Infrared Spectroscopy for the Analysis of Glycan Mixtures
Analytical Chemistry. 2019. Vol. 91, p. 4876-4882. DOI : 10.1021/acs.analchem.9b00659.The Structure of the Protonated Serine Octamer
Journal of the American Chemical Society. 2018-04-11. Vol. 120, num. 24, p. 7554-7560. DOI : 10.1021/jacs.8b02118.| Funded by: |  |