# How to use the analysis GUI

In this section you will learn how to use the graphical user interface. Please visit the youtube tutorial for more informations: https://www.youtube.com/watch?v=McKy_hNy7cg&feature=plcp

## 0) Open GUI.m and click on "run"

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## 1) Load your signal and start the analysis

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Prepare your signal in a .mat file. See User Guide scripts if you have any problem with the input signal.

## 2) Enter Input Parameters

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a) input the value of delta: most likely current drop in nA that you want to detect in an efficient manner (in the demo case 0.2 nA is a typical current blockage value for λ DNA translocation)
b) input the value of sigma: RMS current noise value, can be calculated using std() function of MATLAB on 1000 points of baseline or more
c) input the sampling frequency used when recording the signal in Hz
e) give a name to the folder in which your results will be stored

## 3) Validate Results

Look at the fitted events using the "previous" and "next" buttons, check that the recursive filter found all events and that the CUSUM algorithm fitted all events correctly. Alternatively to the buttons you can use the left and right arrow key of your keyboard.

## 4) Delete unwanted events

If you want to delete an event from the database you can do so by using the delete button or the backspace button of your keyboard. A new temporary database is created. It will be deleted when the program is closed.

## 5) Remove multiple events (1)

You can remove multiple unwanted events by specifying their event numbers. Click on Remove Events in the Tools menu. The specify the event numbers you want to remove in the pop-up window, e.g. 1 2 3 4 5 10 15

## 6) Remove multiple events (2)

Alternatively you can use a time threshold deleting all events longer than the specified threshold. In this case the threshold has to be specified in the used units, e.g. ms. Click on Remove Events Threshold in the Tools menu and specify the length threshold, e.g. 2 for 2ms.

## 7) Save a database

If you want to save a modified database use the menu item Save EventDatabase in the menu Tools. A pop up window will ask you to specify the name of this new database. It is then stored inside your analysis-folder.

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