# Using the Scripts

In this section you will learn how to run an analysis using the scripts.

## 1) load your signal in the workspace

FORMAT of input signal: the input signal need to be in a double of <nx1> ionic current values in nA.

Your input signal MUST contain baseline information, at least 100 points of baseline before and after each event.

## 2) Check your signal by plotting it

Note that the baseline shouldn't contain any sudden jump...

If you have a problem loading the signal please email us! See address on website: http://lben.epfl.ch/page-79460-en.html

## 2) Open main.m and fill in the input parameters

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line 5: give a name to the folder in which your results will be stored
line 6: input the name of the signal you just loaded in the Workspace
line 7: input the value of delta: most likely current drop in nA that you want to detect in an efficient manner (in the demo case 0.2 nA is a typical current blockage value for λ DNA translocation)
line 8: input the value of sigma: RMS current noise value, can be calculated using std() function of MATLAB on 1000 points of baseline or more
line 9: input the sampling frequency used when recording the signal in Hz

## 3) run the main

You can either press the button shown above or write main in the Command Window.

## 4) look at the results

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A .mat file called ExperimentData is created containing all results of an analysis:
EventDatabase, ConcatenatedEvents and ConcatenatedFits and the settings of the analysis.
EventDatabase is a structure array with level and dwell time information for each event (see Event Database for more information) ConcatenatedEvents is a concatenation of all the detected events with 100 points of baseline between each event, ConcatenatedFits are its corresponding fits.

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