The Ecole Polytechnique Fédérale de Lausanne (EPFL) is one of the most dynamic university campuses in Europe and ranks among the top 20 universities worldwide. The EPFL employs 6,000 people supporting the three main missions of the institutions: education, research and innovation. The EPFL campus offers an exceptional working environment at the heart of a community of 16,000 people, including over 10,000 students and 3,500 researchers from 120 different countries.
The LBEM is affiliated with the following graduate groups:
(Molecular Life Sciences)
We have the following openings at the LBEM:
We have a postdoc opening to be hosted in our [email protected] in the area of method development for Cryo-EM.
The center of this research is a Titan Krios 300kV Cold-FEG instrument with a GHz RF cavity below the gun, followed by a probe Cs corrector. Data will be collected on a FalconIV detector or a very high frame-rate hybrid pixel detector.
This instrument invites for experiments, using GHz single electron illumination onto the cryo sample, in order to reduce the beam damage in otherwise conventional single particle cryo-EM with data collection on a FalconIV detector. A separate project is to develop single molecule electron diffraction, similar to but different than ptychography or 4D STEM, exploiting phase contrast in diffraction mode and collecting dose-fractionated electron diffraction as full frames at very high frame rates.
This project is part of a collaborative network with TFS and researchers at the EPFL and other institutes in Switzerland.
Your profile :
Candidates should have experience in high-resolution electron microscopy and a Ph.D. in Physics, Materials Sciences, Electrical Engineering, Life Sciences or related disciplines.
We offer :
The EPFL ranks among the world’s top scientific universities. It is located in Lausanne in Switzerland, a beautiful, vibrant and highly international city on the shores of scenic Lake Geneva, situated within an Alpine setting in the heart of Europe. French is the main language spoken in the city of Lausanne, while English is the main language at the EPFL and in our laboratory. The position comes with a competitive salary. EPFL is committed to being an equal opportunity employer.
The successful postdoc will join a vibrant, collaborative, international, and diverse research team with interdisciplinary expertise in biology, physics, computer science and engineering. Our lab in the Institute of Physics at the EPFL operates outstanding equipment. We develop methods in cryo-EM and apply these to study neurodegeneration. In addition, the Dubochet Center for Imaging in Lausanne provides access to frontier cryo-EM, including another two Titan Krios instruments.
Start date :
The position is available immediately, the start date is flexible.
Applications should include PDF documents with:
(i) Cover letter,
(iii) the contact information of three references.
For questions or additional information, please contact Henning Stahlberg at [email protected]
A PhD student position is available in the Stahlberg laboratory to develop methods in Coherent Electron Diffractive Imaging (CEDI) to study frozen hydrated biological specimens. CEDI is a method that uses a high-end cryo-electron microscope to illuminate a frozen cryo-EM protein sample with a convergent but slightly defocussed electron beam. An aberration corrector in the illumination system of the electron microscope will allow to maintain coherence in the illumination despite the high angle of beam convergence. This beam will traverse the frozen cryo sample and phase-contrasted electron diffraction patterns of the sample will be recorded with a high-speed hybrid pixel detector camera. These patterns will be computationally evaluated with iterative algorithms in order to reconstruct the electron illumination wavefront and the object wavefront. The latter corresponds to the projection image of the specimen. This method will be automated to be applied to a multitude of frozen protein samples, and the ensemble of recorded patterns will be evaluated to obtain the high-resolution 3D structure of the biological protein.
This project will utilize a prototype frontier cryo-electron microscope that will be installed in our laboratory. It will involve advanced electron microscopy optics, aberration correction, and phase contrast diffractive imaging. It will also involve the application of the method to study the 3D structure of an important membrane protein by cryo-EM.
The candidate for this PhD position should hold a Master’s degree in Physics, Electrical Engineering, or a related field (in exceptional cases, a Bachelor’s degree will also be accepted). The lab language is English. The candidate must be accepted by the doctoral school in Physics.
For more information, please contact Henning Stahlberg.
A PhD student position is available in the Stahlberg laboratory to contribute to the development of advanced methods in automated high-resolution structure determination of biological macromolecules by cryo-electron microscopy. The laboratory will setup a frontier cryo-EM instrument in the basement of the Cubotron building. This instrument will be used in to perform real-space imaging and also reciprocal-space electron diffraction data collection. The automated control of the electron beam in diffraction mode in the electron microscope has to be developed. The beam shall expose the sample at various locations, but in synchronization with a high-speed camera that collects images and diffraction patterns at a speed of several thousand frames per second. Data will be recorded at very high speed and very large volumes, so that the data have to be processed in real time to extract the structural information before the noisy data are deleted.
This project will be at the interface between physics, electronic engineering, mathematics and computer sciences. It will involve collaborations with several other laboratories on campus and internationally.
The candidate for this PhD position should hold a Master’s degree in Physics, Electrical Engineering, Computer Sciences, Mathematics, or a related field (in exceptional cases, a Bachelor’s degree will also be accepted). The lab language is English. The candidate must be accepted by the doctoral school in Physics.
For more information, please contact Henning Stahlberg.