Open Positions

The EPFL

The Ecole Polytechnique Fédérale de Lausanne (EPFL) is one of the most dynamic university campuses in Europe and ranks among the top 20 universities worldwide. The EPFL employs 6,000 people supporting the three main missions of the institutions: education, research and innovation. The EPFL campus offers an exceptional working environment at the heart of a community of 16,000 people, including over 10,000 students and 3,500 researchers from 120 different countries.

DCI

The Dubochet Center for Imaging (DCI) is a joint initiative of the EPFL, the University of Lausanne and the University of Geneva. It will be inaugurated with two Titan Krios G4 and one Glacios cryo-electron microscopes, all equipped with Falcon-IV detectors. The Titans will feature Cold-FEG electron sources, and one Titan will have a SelectrisX energy filter. The instruments will run under high automation. Further instruments for the DCI will follow. 

The DCI will have as partner site the CryoGEnic cryo-EM facility at the University of Geneva. In addition, the DCI will closely interact with the EMF cryo-EM facility of the University of Lausanne and the BioEMCIME electron microscopy facilities of the EPFL.

The DCI will use frontier cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) to contribute to life sciences research in the Lemanic region. The DCI will expand its cryo-EM and cryo-ET studies with correlative light and electron microscopy (CLEM), correlative serial block-face scanning electron microscopy or FIB-SEM, and advanced levels of sample preparation and data analysis. The DCI will train the next generation of structural biologists and serve as platform to host workshops and seminars. It will strive to implement the latest developments in the areas of hardware and software.

The DCi will contribute to the [email protected] initiative, and provide cryo-EM analysis to the thriving life sciences research landscape in Lausanne, Geneva, and beyond.  

We have the following openings at the DCI:

We seek a head for the DCI. This person will support the setup of the DCI and its instruments, and be responsible for running the center’s activities in imaging, teaching and outreach. The candidate will train and supervise the center staff, prepare and coordinate the installation of the center’s instruments, manage instrument maintenance and operation, contribute to training and supervision of users in the operation of the instruments, and coordinate projects and instrument assignments to projects.

Your profile :
The candidate should have a Ph.D. in biology, engineering, physics or a related discipline. Extensive experience in cryo-EM and cryo-ET, including sample preparation, instrument setup and alignment, manual and automated data collection, as well as image analysis is expected. The candidate should have a good understanding of biological research and cell and molecular biology. Excellent interpersonal skills as well as excellent communication skills in English are required, together with a service-oriented mindset in interactions with customers of the Center.

We offer :
The EPFL ranks among the world’s top scientific universities. It is located in Lausanne in Switzerland, a beautiful, vibrant and highly international city on the shores of scenic Lake Geneva, situated within an Alpine setting in the heart of Europe. French is the main language spoken in the city of Lausanne, while English is the main language at the EPFL and in at the DCI. The position comes with a competitive salary. EPFL is committed to being an equal opportunity employer.

In the Dubochet Center for Imaging, the candidate will join a center shared by the University of Geneva, the University of Lausanne, and the EPFL, and become part of a vibrant, collaborative, international, and diverse research environment, with interdisciplinary collaborations between biology, physics, computer science and engineering.

Start date :
The position is available immediately, the start date is flexible.

Applications should be sent to [email protected] and include PDF documents with:
(i) Cover letter,
(ii) CV, and
(iii) the contact information of three references.

Work rate :
100%

Application deadline :
All applications received by December 26, 2020 will be considered. The position remains open until filled.

Contact :
For questions or additional information, please contact Henning Stahlberg at [email protected]

We are seeking a Service Engineer for the electron microscopes at the DCI. This person will support the setup of the DCI and its instruments, and perform maintenance and repair tasks on the DCI instruments. In addition, this person may participate in the research routines and customer support of the DCI.

Your profile :
The candidate should have a training in electron microscope maintenance, repair and alignment from a leading electron microscopy manufacturer. The candidate should have a good understanding of computer control and instrument software systems. Participation in student and research projects involving biological research is possible. Excellent interpersonal skills as well as excellent communication skills in English are required, together with a service-oriented mindset in interactions with customers of the Center.

We offer :
The EPFL ranks among the world’s top scientific universities. It is located in Lausanne in Switzerland, a beautiful, vibrant and highly international city on the shores of scenic Lake Geneva, situated within an Alpine setting in the heart of Europe. French is the main language spoken in the city of Lausanne, while English is the main language at the EPFL and in at the DCI. The position comes with a competitive salary. EPFL is committed to being an equal opportunity employer.

In the Dubochet Center for Imaging, the successful applicant will join a center shared by the University of Geneva, the University of Lausanne, and the EPFL, and become part of a vibrant, collaborative, international, and diverse research environment, with interdisciplinary collaborations between biology, physics, computer science and engineering.

Start date :
The position is available immediately, the start date is flexible.

Applications should be sent to [email protected] and include PDF documents with:
(i) Cover letter,
(ii) CV, and
(iii) the contact information of three references.

Work rate :
100%

Application deadline :
All applications received by December 26, 2020 will be considered. The position remains open until filled.

Contact :
For questions or additional information, please contact Henning Stahlberg at [email protected]

We are inviting applications for the position of a Cryo-EM Expert at the DCI. This is a service position to support projects of the clients of the DCI. This person will operate the DCI instruments and perform structural investigations of biological specimens using cryo-EM. The person will also participate in the center’s activities in teaching and outreach. The candidate will contribute to microscope alignment, sample preparation, and manual and automated EM data collection. Optional contribution or performance of computational data analysis is welcome.

Your profile :
The candidate should have an education in biology, engineering, physics or a related discipline. Extensive experience in cryo-EM and cryo-ET, including sample preparation, instrument setup and alignment, and manual and automated data collection is expected. The candidate should have a basic understanding of biological research and of cell and molecular biology. Excellent interpersonal skills as well as excellent communication skills in English are required, together with a service-oriented mindset in interactions with customers of the Center.

We offer :
The EPFL ranks among the world’s top scientific universities. It is located in Lausanne in Switzerland, a beautiful, vibrant and highly international city on the shores of scenic Lake Geneva, situated within an Alpine setting in the heart of Europe. French is the main language spoken in the city of Lausanne, while English is the main language at the EPFL and in at the DCI. The position comes with a competitive salary. EPFL is committed to being an equal opportunity employer.

In the Dubochet Center for Imaging, the candidate will join a center shared by the University of Geneva, the University of Lausanne, and the EPFL, and become part of a vibrant, collaborative, international, and diverse research environment, with interdisciplinary collaborations between biology, physics, computer science and engineering.

Start date :
The position is available immediately, the start date is flexible.

Applications should be sent to [email protected] and include PDF documents with:
(i) Cover letter,
(ii) CV, and
(iii) the contact information of three references.

Work rate :
100%

Application deadline :
All applications received by December 26, 2020 will be considered. The position remains open until filled.

Contact :
For questions or additional information, please contact Henning Stahlberg at [email protected]


LBEM

Our Laboratory of Biological Electron Microscopy (LBEM) in the Physics Institute of the Basic Sciences division at the EPFL is headed by Henning Stahlberg. The LBEM will setup its own electron microscopes, which will be primarily dedicated to method development. The LBEM will be a client to the services of the DCI, so that the above listed instruments of the DCI are equally accessible to us. The lab language is English, while the city of Lausanne is in the French-speaking part of Switzerland.  

The LBEM is affiliated with the following graduate groups:

EDPY
(Physics)

EDMS
(Molecular Life Sciences)
EDNE
(Neurosciences)

 

We have the following openings at the LBEM:

We have a postdoc opening to be hosted in our [email protected] in the area of structural investigations of biological macromolecules by cryo-electron microscopy (cryo-EM), with a focus on neurodegeneration and Parkinson’s disease.

The project aims to study the structure, function and intracellular mechanisms of several proteins involved in familial forms of Parkinson’s disease, such as alpha-synuclein or LRRK2. Proteins will be expressed and purified on campus and prepared for cryo-EM investigations. Data collection will be done with outstanding in-house equipment, which includes latest generation 300kV instruments, energy filtration and large-area direct electron detectors. Data analysis will be done with extensive IT equipment, using single particle cryo-EM, cryo-electron tomography or CLEM methods.

Your profile :
Candidates should have a Ph.D. in Biology or Physics or related disciplines. The research is expected to involve a combination of some wetlab work, cryo-EM and image processing. Knowledge in cryo-EM sample preparation, data collection and image processing is required.

We offer :
The EPFL ranks among the world’s top scientific universities. It is located in Lausanne in Switzerland, a beautiful, vibrant and highly international city on the shores of scenic Lake Geneva, situated within an Alpine setting in the heart of Europe. French is the main language spoken in the city of Lausanne, while English is the main language at the EPFL and in our laboratory. The position comes with a competitive salary. EPFL is committed to being an equal opportunity employer.

The successful postdoc will join a vibrant, collaborative, international, and diverse research team with interdisciplinary expertise in biology, physics, computer science and engineering. The focus of research activity at the LBEM is centered around neurodegeneration and method development in cryo-EM. The LBEM is a newly established research lab at the Institute of Physics at the EPFL, we will enjoy unique state-of-the-art instrumentation. In addition, the future Dubochet Center for Imaging in Lausanne will provide outstanding access possibilities to cryo-EM.

More information about both of our research groups, including other active research themes, can be found in the following links:
https://www.epfl.ch/labs/lbem/

Start date :
The position is available immediately, funding is available, and the start date is flexible.

Applications should include PDF documents with:
(i) Cover letter,
(ii) CV,
(iii) the contact information of three references.

Contact :
For questions or additional information, please contact Henning Stahlberg at [email protected]

We have a postdoc opening to be hosted in our [email protected] in the area of hardware and software development for high-speed data collection in diffractive electron imaging.

The project aims to develop a system that can determine the structure of frozen hydrated proteins with a cryo-transmission electron microscope, using a high-speed hybrid pixel detector camera and diffractive imaging approaches. Proteins will be frozen in thin, free-standing ice layers as single particles, which is the same method that is used for single particle cryo-EM. However, data will be collected with an aberration corrected high-end cryo-EM instrument in diffraction mode, using a prototype high-speed camera. The project involves readout of the camera at very high frame rates and online processing of the recorded data during data collection. This will involve GPU or FPGA architectures, and the development and implementation of novel algorithms.

Your profile :
Candidates should have a Ph.D. in Physics or Electrical Engineering or Computer Sciences or related disciplines. The research is expected to involve a combination of some electronics hardware and software development. Knowledge in cryo-EM is welcome but not required.

We offer :
The EPFL ranks among the world’s top scientific universities. It is located in Lausanne in Switzerland, a beautiful, vibrant and highly international city on the shores of scenic Lake Geneva, situated within an Alpine setting in the heart of Europe. French is the main language spoken in the city of Lausanne, while English is the main language at the EPFL and in our laboratory. The position comes with a competitive salary. EPFL is committed to being an equal opportunity employer.

The successful postdoc will join a vibrant, collaborative, international, and diverse research team with interdisciplinary expertise in biology, physics, computer science and engineering. The focus of research activity at the LBEM is centered around neurodegeneration and method development in cryo-EM. The LBEM is a newly established research lab at the Institute of Physics at the EPFL, we will enjoy unique state-of-the-art instrumentation. In addition, the future Dubochet Center for Imaging in Lausanne will provide outstanding access possibilities to cryo-EM.

Start date :
The position is available immediately, funding is available, and the start date is flexible.

Applications should include PDF documents with:
(i) Cover letter,
(ii) CV,
(iii) the contact information of three references.

Contact :
For questions or additional information, please contact Henning Stahlberg at [email protected]

A PhD student position is available in the Stahlberg laboratory to develop methods in Coherent Electron Diffractive Imaging (CEDI) to study frozen hydrated biological specimens. CEDI is a method that uses a high-end cryo-electron microscope to illuminate a frozen cryo-EM protein sample with a convergent but slightly defocussed electron beam. An aberration corrector in the illumination system of the electron microscope will allow to maintain coherence in the illumination despite the high angle of beam convergence. This beam will traverse the frozen cryo sample and phase-contrasted electron diffraction patterns of the sample will be recorded with a high-speed hybrid pixel detector camera. These patterns will be computationally evaluated with iterative algorithms in order to reconstruct the electron illumination wavefront and the object wavefront. The latter corresponds to the projection image of the specimen. This method will be automated to be applied to a multitude of frozen protein samples, and the ensemble of recorded patterns will be evaluated to obtain the high-resolution 3D structure of the biological protein. 

This project will utilize a prototype frontier cryo-electron microscope that will be installed by our laboratory in the Cubotron building. It will involve advanced electron microscopy optics, including aberration correction, phase contrast ptychography optics methods, and diffractive imaging. It will also involve the application of the method to study the 3D structure of an important membrane protein by cryo-EM. 

The candidate for this PhD position should hold a Master’s degree in Physics, Electrical Engineering, or a related field (in exceptional cases, a Bachelor’s degree will also be accepted). The lab language is English. The candidate must be accepted by the doctoral school in Physics.

For more information, please contact Henning Stahlberg.

A PhD student position is available in the Stahlberg laboratory to contribute to the development of advanced methods in automated high-resolution structure determination of biological macromolecules by cryo-electron microscopy. The laboratory will setup a frontier cryo-EM instrument in the basement of the Cubotron building. This instrument will be used in to perform real-space imaging and also reciprocal-space electron diffraction data collection. The automated control of the electron beam in diffraction mode in the electron microscope has to be developed. The beam shall expose the sample at various locations, but in synchronization with a high-speed camera that collects images and diffraction patterns at a speed of several thousand frames per second. Data will be recorded at very high speed and very large volumes, so that the data have to be processed in real time to extract the structural information before the noisy data are deleted. 

This project will be at the interface between physics, electronic engineering, mathematics and computer sciences. It will involve collaborations with several other laboratories on campus and internationally.  

The candidate for this PhD position should hold a Master’s degree in Physics, Electrical Engineering, Computer Sciences, Mathematics, or a related field (in exceptional cases, a Bachelor’s degree will also be accepted). The lab language is English. The candidate must be accepted by the doctoral school in Physics.

For more information, please contact Henning Stahlberg.